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Detection of cellular damage by hydrogen peroxide using SV40-T2 cells on shear horizontal surface acoustic wave (SH-SAW) sensor.
Ultrasonics. 2014 Aug; 54(6):1430-8.U

Abstract

The rat lung epithelial cell line SV40-T2 was used to develop a cellular biosensing system to assay for environmental toxicants. The novel approach on which this system is based involves direct attachment of cultured rat or human cells onto a cell-adhesive matrix on the device through which shear horizontal surface acoustic waves (SH-SAW) are transmitted using 50 MHz SAW resonator. This novel design enables sensitive monitoring of changes of the electrophysical characteristics of cells, such as their conductivity and relative permittivity. A time-dependent change of phase of SAW and change of insertion loss (change of amplitude) were observed when the cells were treated with 0.5 or 1.0 mM H2O2. The change of insertion loss was biphasic, with an early phase (1-3 h) and a late phase (3-6 h). The late phase coincided with the destruction of cell-cell tight junctions detected by measurement of the transepithelial electrical resistance and paracellular permeability; in contrast, the early phase coincided with the destruction of intracellular actin filaments by H2O2. The early-phase effect of H2O2 on phase shift may be attributable to the change of intracellular permittivity by a change of cellular polarity. Immunofluorescence microscopy showed the disappearance of zonula occludens protein 1 from the region of cell-cell contact. These results suggest the correlation between the change of insertion loss as an SAW parameter and the destruction of tight junctions of the cells on the SH-SAW device in the late phase.

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Authors+Show Affiliations

Higashiyama T
Faculty of Science and Technology, Department of Intelligent Machines and System Engineering, Hirosaki University, Japan.
Katsuyama A
Faculty of Agriculture and Life Science, Department of Biochemistry and Molecular Biology, Hirosaki University, 3 Bunkyo-cho, Hirosaki 036-8561, Japan.
Otori H
Faculty of Science and Technology, Department of Intelligent Machines and System Engineering, Hirosaki University, Japan.
Kamimura T
Faculty of Agriculture and Life Science, Department of Biochemistry and Molecular Biology, Hirosaki University, 3 Bunkyo-cho, Hirosaki 036-8561, Japan.
Uehara A
Faculty of Science and Technology, Department of Intelligent Machines and System Engineering, Hirosaki University, Japan.
Kainuma M
Faculty of Science and Technology, Department of Intelligent Machines and System Engineering, Hirosaki University, Japan.
Takumi R
Faculty of Agriculture and Life Science, Department of Biochemistry and Molecular Biology, Hirosaki University, 3 Bunkyo-cho, Hirosaki 036-8561, Japan.
Kudo Y
Faculty of Agriculture and Life Science, Department of Biochemistry and Molecular Biology, Hirosaki University, 3 Bunkyo-cho, Hirosaki 036-8561, Japan.
Ebina M
Faculty of Agriculture and Life Science, Department of Biochemistry and Molecular Biology, Hirosaki University, 3 Bunkyo-cho, Hirosaki 036-8561, Japan.
Mochitate K
National Institute for Environmental Studies, 16-2, Onogawa, Tsukuba-shi, Ibaraki 305-8506, Japan.
Kon T
RIVER ELETEC Corporation, 2-1-11 Fujimigaoka, Nirasaki, Yamanashi 407-8502, Japan.
Furuya Y
Faculty of Science and Technology, Department of Intelligent Machines and System Engineering, Hirosaki University, Japan.
Kikuchi H
Faculty of Agriculture and Life Science, Department of Biochemistry and Molecular Biology, Hirosaki University, 3 Bunkyo-cho, Hirosaki 036-8561, Japan. Electronic address: hkikuchi@cc.hirosaki-u.ac.jp.

MeSH

AcousticsActinsAnimalsBiosensing TechniquesCell LineCells, CulturedElectric ImpedanceEpithelial CellsHydrogen PeroxideMicroscopy, FluorescenceRatsShear StrengthTight JunctionsUltrasonography

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24835005

Citation

Higashiyama, Takumi, et al. "Detection of Cellular Damage By Hydrogen Peroxide Using SV40-T2 Cells On Shear Horizontal Surface Acoustic Wave (SH-SAW) Sensor." Ultrasonics, vol. 54, no. 6, 2014, pp. 1430-8.
Higashiyama T, Katsuyama A, Otori H, et al. Detection of cellular damage by hydrogen peroxide using SV40-T2 cells on shear horizontal surface acoustic wave (SH-SAW) sensor. Ultrasonics. 2014;54(6):1430-8.
Higashiyama, T., Katsuyama, A., Otori, H., Kamimura, T., Uehara, A., Kainuma, M., Takumi, R., Kudo, Y., Ebina, M., Mochitate, K., Kon, T., Furuya, Y., & Kikuchi, H. (2014). Detection of cellular damage by hydrogen peroxide using SV40-T2 cells on shear horizontal surface acoustic wave (SH-SAW) sensor. Ultrasonics, 54(6), 1430-8. https://doi.org/10.1016/j.ultras.2014.04.026
Higashiyama T, et al. Detection of Cellular Damage By Hydrogen Peroxide Using SV40-T2 Cells On Shear Horizontal Surface Acoustic Wave (SH-SAW) Sensor. Ultrasonics. 2014;54(6):1430-8. PubMed PMID: 24835005.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of cellular damage by hydrogen peroxide using SV40-T2 cells on shear horizontal surface acoustic wave (SH-SAW) sensor. AU - Higashiyama,Takumi, AU - Katsuyama,Akihiro, AU - Otori,Hideki, AU - Kamimura,Toru, AU - Uehara,Atsushi, AU - Kainuma,Miho, AU - Takumi,Ryo, AU - Kudo,Yukako, AU - Ebina,Masayuki, AU - Mochitate,Katsumi, AU - Kon,Tasuku, AU - Furuya,Yasubumi, AU - Kikuchi,Hideaki, Y1 - 2014/05/08/ PY - 2013/12/10/received PY - 2014/03/26/revised PY - 2014/04/25/accepted PY - 2014/5/20/entrez PY - 2014/5/20/pubmed PY - 2014/7/22/medline KW - Epithelial cell KW - Hydrogen peroxide KW - Shear horizontal surface acoustic wave KW - Tight junction KW - Transepithelial electrical resistance SP - 1430 EP - 8 JF - Ultrasonics JO - Ultrasonics VL - 54 IS - 6 N2 - The rat lung epithelial cell line SV40-T2 was used to develop a cellular biosensing system to assay for environmental toxicants. The novel approach on which this system is based involves direct attachment of cultured rat or human cells onto a cell-adhesive matrix on the device through which shear horizontal surface acoustic waves (SH-SAW) are transmitted using 50 MHz SAW resonator. This novel design enables sensitive monitoring of changes of the electrophysical characteristics of cells, such as their conductivity and relative permittivity. A time-dependent change of phase of SAW and change of insertion loss (change of amplitude) were observed when the cells were treated with 0.5 or 1.0 mM H2O2. The change of insertion loss was biphasic, with an early phase (1-3 h) and a late phase (3-6 h). The late phase coincided with the destruction of cell-cell tight junctions detected by measurement of the transepithelial electrical resistance and paracellular permeability; in contrast, the early phase coincided with the destruction of intracellular actin filaments by H2O2. The early-phase effect of H2O2 on phase shift may be attributable to the change of intracellular permittivity by a change of cellular polarity. Immunofluorescence microscopy showed the disappearance of zonula occludens protein 1 from the region of cell-cell contact. These results suggest the correlation between the change of insertion loss as an SAW parameter and the destruction of tight junctions of the cells on the SH-SAW device in the late phase. SN - 1874-9968 UR - https://www.unboundmedicine.com/medline/citation/24835005/Detection_of_cellular_damage_by_hydrogen_peroxide_using_SV40_T2_cells_on_shear_horizontal_surface_acoustic_wave__SH_SAW__sensor_ DB - PRIME DP - Unbound Medicine ER -