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Beta-caryophyllene modulates expression of stress response genes and mediates longevity in Caenorhabditis elegans.
Exp Gerontol 2014; 57:81-95EG

Abstract

Beta-caryophyllene (BCP) is a natural bicyclic sesquiterpene and is a FDA approved food additive, found as an active ingredient in essential oils of numerous edible plants. It possesses a wide range of biological activities including anti-oxidant, anti-inflammatory, anti-cancerous and local anesthetic actions. We used the well established Caenorhabditis elegans model system to elucidate the stress modulatory and lifespan prolonging action of BCP. The present study for the first time reports the lifespan extension and stress modulation potential of BCP in C. elegans. Upon evaluation, it was found that 50μM dose of BCP increased the lifespan of C. elegans by over 22% (P≤0.0001) and significantly reduced intracellular free radical levels, maintaining cellular redox homeostasis. Moreover, the results suggest that BCP modulates feeding behavior, pharyngeal pumping and body size effectively. Further, this compound also exhibited significant reduction in intestinal lipofuscin levels. In the present investigation, we have predicted possible biological molecular targets for BCP using molecular docking approaches and BCP was found to have interaction with SIR-2.1, SKN-1 and DAF-16. The prediction was further validated in vivo using mutants and transgenic strains unraveling underlying genetic mechanism. It was observed that BCP increased lifespan of mev-1 and daf-16 but failed to augment lifespan in eat-2, sir-2.1 and skn-1 mutants. Relative quantification of mRNA demonstrated that several genes regulating oxidative stress, xenobiotic detoxification and longevity were modulated by BCP treatment. The study unravels the involvement of multiple signaling pathways in BCP mediated lifespan extension.

Authors+Show Affiliations

Department of Microbial Technology and Nematology, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow - 226 015, India.No affiliation info availableDepartment of Microbial Technology and Nematology, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow - 226 015, India.Department of Microbial Technology and Nematology, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow - 226 015, India.Department of Microbial Technology and Nematology, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow - 226 015, India.Department of Microbial Technology and Nematology, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow - 226 015, India.Department of Microbial Technology and Nematology, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow - 226 015, India. Electronic address: r.pandey@cimap.res.in.

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

24835194

Citation

Pant, Aakanksha, et al. "Beta-caryophyllene Modulates Expression of Stress Response Genes and Mediates Longevity in Caenorhabditis Elegans." Experimental Gerontology, vol. 57, 2014, pp. 81-95.
Pant A, Mishra V, Saikia SK, et al. Beta-caryophyllene modulates expression of stress response genes and mediates longevity in Caenorhabditis elegans. Exp Gerontol. 2014;57:81-95.
Pant, A., Mishra, V., Saikia, S. K., Shukla, V., Asthana, J., Akhoon, B. A., & Pandey, R. (2014). Beta-caryophyllene modulates expression of stress response genes and mediates longevity in Caenorhabditis elegans. Experimental Gerontology, 57, pp. 81-95. doi:10.1016/j.exger.2014.05.007.
Pant A, et al. Beta-caryophyllene Modulates Expression of Stress Response Genes and Mediates Longevity in Caenorhabditis Elegans. Exp Gerontol. 2014;57:81-95. PubMed PMID: 24835194.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Beta-caryophyllene modulates expression of stress response genes and mediates longevity in Caenorhabditis elegans. AU - Pant,Aakanksha, AU - Mishra,Vikas, AU - Saikia,Shilpi K, AU - Shukla,Virendra, AU - Asthana,Jyotsna, AU - Akhoon,Bashir A, AU - Pandey,Rakesh, Y1 - 2014/05/15/ PY - 2014/01/30/received PY - 2014/05/07/revised PY - 2014/05/08/accepted PY - 2014/5/20/entrez PY - 2014/5/20/pubmed PY - 2015/5/27/medline KW - Anti-aging KW - Beta-caryophyllene KW - Caenorhabditis elegans KW - Lifespan KW - Phytomolecule SP - 81 EP - 95 JF - Experimental gerontology JO - Exp. Gerontol. VL - 57 N2 - Beta-caryophyllene (BCP) is a natural bicyclic sesquiterpene and is a FDA approved food additive, found as an active ingredient in essential oils of numerous edible plants. It possesses a wide range of biological activities including anti-oxidant, anti-inflammatory, anti-cancerous and local anesthetic actions. We used the well established Caenorhabditis elegans model system to elucidate the stress modulatory and lifespan prolonging action of BCP. The present study for the first time reports the lifespan extension and stress modulation potential of BCP in C. elegans. Upon evaluation, it was found that 50μM dose of BCP increased the lifespan of C. elegans by over 22% (P≤0.0001) and significantly reduced intracellular free radical levels, maintaining cellular redox homeostasis. Moreover, the results suggest that BCP modulates feeding behavior, pharyngeal pumping and body size effectively. Further, this compound also exhibited significant reduction in intestinal lipofuscin levels. In the present investigation, we have predicted possible biological molecular targets for BCP using molecular docking approaches and BCP was found to have interaction with SIR-2.1, SKN-1 and DAF-16. The prediction was further validated in vivo using mutants and transgenic strains unraveling underlying genetic mechanism. It was observed that BCP increased lifespan of mev-1 and daf-16 but failed to augment lifespan in eat-2, sir-2.1 and skn-1 mutants. Relative quantification of mRNA demonstrated that several genes regulating oxidative stress, xenobiotic detoxification and longevity were modulated by BCP treatment. The study unravels the involvement of multiple signaling pathways in BCP mediated lifespan extension. SN - 1873-6815 UR - https://www.unboundmedicine.com/medline/citation/24835194/Beta_caryophyllene_modulates_expression_of_stress_response_genes_and_mediates_longevity_in_Caenorhabditis_elegans_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0531-5565(14)00155-7 DB - PRIME DP - Unbound Medicine ER -