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Highly sensitive homogenous chemiluminescence immunoassay using gold nanoparticles as label.
Spectrochim Acta A Mol Biomol Spectrosc. 2014 Oct 15; 131:243-8.SA

Abstract

Homogeneous immunoassay is becoming more and more attractive for modern medical diagnosis because it is superior to heterogeneous immunoassay in sample and reagent consumption, analysis time, portability and disposability. Herein, a universal platform for homogeneous immunoassay, using human immunoglobulin G (IgG) as a model analyte, has been developed. This assay relies upon the catalytic activity of gold nanoparticles (AuNPs) on luminol-AgNO3 chemiluminescence (CL) reaction. The immunoreaction of antigen and antibody can induce the aggregation of antibody-functionalized AuNPs, and after aggregation the catalytic activity of AuNPs on luminol-AgNO3 CL reaction is greatly enhanced. Without any separation steps, a CL signal is generated upon addition of a trigger solution, and the CL intensity is directly correlated to the quantity of IgG. The detection limit of IgG was estimated to be as low as 3pg/mL, and the sensitivity was better than that of the reported AuNPs-based CL immunoassay for IgG.

Authors+Show Affiliations

Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, China.Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, China.Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, China.Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, China. Electronic address: libaoxin@snnu.edu.cn.

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24835732

Citation

Luo, Jing, et al. "Highly Sensitive Homogenous Chemiluminescence Immunoassay Using Gold Nanoparticles as Label." Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy, vol. 131, 2014, pp. 243-8.
Luo J, Cui X, Liu W, et al. Highly sensitive homogenous chemiluminescence immunoassay using gold nanoparticles as label. Spectrochim Acta A Mol Biomol Spectrosc. 2014;131:243-8.
Luo, J., Cui, X., Liu, W., & Li, B. (2014). Highly sensitive homogenous chemiluminescence immunoassay using gold nanoparticles as label. Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy, 131, 243-8. https://doi.org/10.1016/j.saa.2014.04.076
Luo J, et al. Highly Sensitive Homogenous Chemiluminescence Immunoassay Using Gold Nanoparticles as Label. Spectrochim Acta A Mol Biomol Spectrosc. 2014 Oct 15;131:243-8. PubMed PMID: 24835732.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Highly sensitive homogenous chemiluminescence immunoassay using gold nanoparticles as label. AU - Luo,Jing, AU - Cui,Xiang, AU - Liu,Wei, AU - Li,Baoxin, Y1 - 2014/04/26/ PY - 2014/03/12/received PY - 2014/04/09/revised PY - 2014/04/17/accepted PY - 2014/5/20/entrez PY - 2014/5/20/pubmed PY - 2015/2/3/medline KW - Chemiluminescence KW - Gold nanoparticles KW - Homogeneous immunoassay SP - 243 EP - 8 JF - Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy JO - Spectrochim Acta A Mol Biomol Spectrosc VL - 131 N2 - Homogeneous immunoassay is becoming more and more attractive for modern medical diagnosis because it is superior to heterogeneous immunoassay in sample and reagent consumption, analysis time, portability and disposability. Herein, a universal platform for homogeneous immunoassay, using human immunoglobulin G (IgG) as a model analyte, has been developed. This assay relies upon the catalytic activity of gold nanoparticles (AuNPs) on luminol-AgNO3 chemiluminescence (CL) reaction. The immunoreaction of antigen and antibody can induce the aggregation of antibody-functionalized AuNPs, and after aggregation the catalytic activity of AuNPs on luminol-AgNO3 CL reaction is greatly enhanced. Without any separation steps, a CL signal is generated upon addition of a trigger solution, and the CL intensity is directly correlated to the quantity of IgG. The detection limit of IgG was estimated to be as low as 3pg/mL, and the sensitivity was better than that of the reported AuNPs-based CL immunoassay for IgG. SN - 1873-3557 UR - https://www.unboundmedicine.com/medline/citation/24835732/Highly_sensitive_homogenous_chemiluminescence_immunoassay_using_gold_nanoparticles_as_label_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1386-1425(14)00649-0 DB - PRIME DP - Unbound Medicine ER -