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Typing of Panton-Valentine leukocidin-encoding phages carried by methicillin-susceptible and methicillin-resistant Staphylococcus aureus from Italy.
Clin Microbiol Infect 2014; 20(11):O840-6CM

Abstract

Panton-Valentine leukocidin (PVL) is the hallmark of community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) but can also be found in methicillin-susceptible S. aureus (MSSA) sharing pathogenic and epidemiological characteristics of CA-MRSA. PVL is encoded by two co-transcribed genes that are carried by different staphylococcal bacteriophages. We applied an extended PCR-based typing scheme for the identification of two morphological groups (elongated-head group and icosahedral-head group I phages) and specific PVL phage types in S. aureus isolates recovered in Italy. We examined 48 PVL-positive isolates (25 MSSA and 23 MRSA) collected from different hospital laboratories from April 2005 to May 2011. spa typing, multilocus sequence typing and staphylococcal cassette chromosome mec typing were applied to categorize the isolates. Phage typeability was 48.0% in MSSA and 91.3% in MRSA, highlighting the limitation of the PCR typing scheme when applied to PVL-positive MSSA. Five different PVL phages and two variants of a known phage were detected, the most prevalent being ΦSa2usa, recovered in 15 out of 48 (31.2%) isolates, and carried by both MSSA and MRSA belonging to CC8 and CC5. The recently described ΦTCH60 was recovered in four isolates. A PVL phage (ΦSa119) from an ST772 MRSA, that was not detected using the previous typing scheme, was sequenced, and new primers were designed for the identification of the icosahedral-head group II PVL phages present in ST772 and ST59 MRSA. A comprehensive PVL-phage typing can contribute to the understanding of the epidemiology and evolution of PVL-positive MSSA and MRSA.

Authors+Show Affiliations

Department of Infectious, Parasitic and Immune-mediated Diseases, Istituto Superiore di Sanità, Rome, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24835735

Citation

Sanchini, A, et al. "Typing of Panton-Valentine Leukocidin-encoding Phages Carried By Methicillin-susceptible and Methicillin-resistant Staphylococcus Aureus From Italy." Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases, vol. 20, no. 11, 2014, pp. O840-6.
Sanchini A, Del Grosso M, Villa L, et al. Typing of Panton-Valentine leukocidin-encoding phages carried by methicillin-susceptible and methicillin-resistant Staphylococcus aureus from Italy. Clin Microbiol Infect. 2014;20(11):O840-6.
Sanchini, A., Del Grosso, M., Villa, L., Ammendolia, M. G., Superti, F., Monaco, M., & Pantosti, A. (2014). Typing of Panton-Valentine leukocidin-encoding phages carried by methicillin-susceptible and methicillin-resistant Staphylococcus aureus from Italy. Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases, 20(11), pp. O840-6. doi:10.1111/1469-0691.12679.
Sanchini A, et al. Typing of Panton-Valentine Leukocidin-encoding Phages Carried By Methicillin-susceptible and Methicillin-resistant Staphylococcus Aureus From Italy. Clin Microbiol Infect. 2014;20(11):O840-6. PubMed PMID: 24835735.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Typing of Panton-Valentine leukocidin-encoding phages carried by methicillin-susceptible and methicillin-resistant Staphylococcus aureus from Italy. AU - Sanchini,A, AU - Del Grosso,M, AU - Villa,L, AU - Ammendolia,M G, AU - Superti,F, AU - Monaco,M, AU - Pantosti,A, Y1 - 2014/06/14/ PY - 2013/12/30/received PY - 2014/03/24/revised PY - 2014/05/12/accepted PY - 2014/5/20/entrez PY - 2014/5/20/pubmed PY - 2015/8/5/medline KW - Community-acquired methicillin-resistant Staphylococcus aureus KW - Panton-Valentine leukocidin KW - ST772 KW - methicillin-susceptible Staphylococcus aureus KW - phage typing SP - O840 EP - 6 JF - Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases JO - Clin. Microbiol. Infect. VL - 20 IS - 11 N2 - Panton-Valentine leukocidin (PVL) is the hallmark of community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) but can also be found in methicillin-susceptible S. aureus (MSSA) sharing pathogenic and epidemiological characteristics of CA-MRSA. PVL is encoded by two co-transcribed genes that are carried by different staphylococcal bacteriophages. We applied an extended PCR-based typing scheme for the identification of two morphological groups (elongated-head group and icosahedral-head group I phages) and specific PVL phage types in S. aureus isolates recovered in Italy. We examined 48 PVL-positive isolates (25 MSSA and 23 MRSA) collected from different hospital laboratories from April 2005 to May 2011. spa typing, multilocus sequence typing and staphylococcal cassette chromosome mec typing were applied to categorize the isolates. Phage typeability was 48.0% in MSSA and 91.3% in MRSA, highlighting the limitation of the PCR typing scheme when applied to PVL-positive MSSA. Five different PVL phages and two variants of a known phage were detected, the most prevalent being ΦSa2usa, recovered in 15 out of 48 (31.2%) isolates, and carried by both MSSA and MRSA belonging to CC8 and CC5. The recently described ΦTCH60 was recovered in four isolates. A PVL phage (ΦSa119) from an ST772 MRSA, that was not detected using the previous typing scheme, was sequenced, and new primers were designed for the identification of the icosahedral-head group II PVL phages present in ST772 and ST59 MRSA. A comprehensive PVL-phage typing can contribute to the understanding of the epidemiology and evolution of PVL-positive MSSA and MRSA. SN - 1469-0691 UR - https://www.unboundmedicine.com/medline/citation/24835735/Typing_of_Panton_Valentine_leukocidin_encoding_phages_carried_by_methicillin_susceptible_and_methicillin_resistant_Staphylococcus_aureus_from_Italy_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1198-743X(14)65336-2 DB - PRIME DP - Unbound Medicine ER -