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Monoclonal antibodies against spore antigens of Bacillus anthracis.
FEMS Microbiol Immunol 1988; 1(3):169-78FM

Abstract

A murine monoclonal antibody produced against heat inactivated spores of Bacillus anthracis Ames, reacted with live or inactivated spores of several anthrax strains in indirect immunofluorescence (IF) tests. The reactive anthrax strain gave only a moderate degree of reaction. No staining of anthrax vegetative cells was observed. The monoclonal did not react with spores of non-anthrax Bacillus strains that gave cross reactions with mouse hyperimmune antiserum raised against Ames spores. The staining of individual spores in B. anthracis preparations was more heterogeneous with the monoclonal antibody than with the hyperimmune serum. Evidence is produced that the epitope for this monoclonal is not stable during long-term storage of inactivated spore preparations, and is not fully available for reaction with antibody until late in spore maturation. The monoclonal did not react by immunoblotting (Western blotting) of spore extracts. A monoclonal antibody produced against Ames spore extracts reacted with about 1% of Ames spores in IF tests, but not reproducible reactions with other anthrax strains were recorded. This monoclonal interacted with three bands in Western blots of anthrax spore extracts.

Authors+Show Affiliations

Chemical Defence Establishment, Salisbury, Wiltshire, U.K.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

2483677

Citation

Phillips, A P., et al. "Monoclonal Antibodies Against Spore Antigens of Bacillus Anthracis." FEMS Microbiology Immunology, vol. 1, no. 3, 1988, pp. 169-78.
Phillips AP, Campbell AM, Quinn R. Monoclonal antibodies against spore antigens of Bacillus anthracis. FEMS Microbiol Immunol. 1988;1(3):169-78.
Phillips, A. P., Campbell, A. M., & Quinn, R. (1988). Monoclonal antibodies against spore antigens of Bacillus anthracis. FEMS Microbiology Immunology, 1(3), pp. 169-78.
Phillips AP, Campbell AM, Quinn R. Monoclonal Antibodies Against Spore Antigens of Bacillus Anthracis. FEMS Microbiol Immunol. 1988;1(3):169-78. PubMed PMID: 2483677.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Monoclonal antibodies against spore antigens of Bacillus anthracis. AU - Phillips,A P, AU - Campbell,A M, AU - Quinn,R, PY - 1988/12/1/pubmed PY - 1988/12/1/medline PY - 1988/12/1/entrez SP - 169 EP - 78 JF - FEMS microbiology immunology JO - FEMS Microbiol Immunol VL - 1 IS - 3 N2 - A murine monoclonal antibody produced against heat inactivated spores of Bacillus anthracis Ames, reacted with live or inactivated spores of several anthrax strains in indirect immunofluorescence (IF) tests. The reactive anthrax strain gave only a moderate degree of reaction. No staining of anthrax vegetative cells was observed. The monoclonal did not react with spores of non-anthrax Bacillus strains that gave cross reactions with mouse hyperimmune antiserum raised against Ames spores. The staining of individual spores in B. anthracis preparations was more heterogeneous with the monoclonal antibody than with the hyperimmune serum. Evidence is produced that the epitope for this monoclonal is not stable during long-term storage of inactivated spore preparations, and is not fully available for reaction with antibody until late in spore maturation. The monoclonal did not react by immunoblotting (Western blotting) of spore extracts. A monoclonal antibody produced against Ames spore extracts reacted with about 1% of Ames spores in IF tests, but not reproducible reactions with other anthrax strains were recorded. This monoclonal interacted with three bands in Western blots of anthrax spore extracts. SN - 0920-8534 UR - https://www.unboundmedicine.com/medline/citation/2483677/Monoclonal_antibodies_against_spore_antigens_of_Bacillus_anthracis_ L2 - https://www.lens.org/lens/search?q=citation_id:2483677 DB - PRIME DP - Unbound Medicine ER -