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Sublethal toxicity of carbofuran on the African catfish Clarias gariepinus: Hormonal, enzymatic and antioxidant responses.
Ecotoxicol Environ Saf. 2014 Aug; 106:33-9.EE

Abstract

The present study examined the impacts of carbofuran on endocrinology of the catfish, Clarias gariepinus, for the first time and evaluated cortisol (CRT), triiodothyronine (T3), thyroxin (T4), 17β-estradiol (E2) and testosterone (TST) and the oxidative stress markers including SOD, CAT, GSTs, GSH. The toxic effects on the metabolic enzymes, G6PDH and LDH, in addition to lipid peroxidation (LPO) and DNA damage as biomarkers in Nile catfish, to sublethal exposures of carbofuran (0.16 and 0.49mg/L, for 35 days) were studied. Statistically significant differences between selected parameters between control and carbofuran-treated fish were recorded. Carbofuran caused a significant (p<0.05) increase in CRT and T3 levels; the mean levels of T4, TST, E2 exhibited significant decreases (p<0.05) in carbofuran-treated fish. Toxicity of carbofuran on liver, kidney, gills, gonads and muscles after 35 days of exposure was found. Glycogen levels showed a highly significant decrease in liver and gills (p< 0.001), a significant decrease (p< 0.05) in kidney and muscles, and insignificant changes (p>0.05) in gonads of treated fish. The two metabolic enzymes G6PDH and LDH in all tissues exhibited significant decreases (p<0.05) in treated fish. SOD, CAT, GSH and GST levels showed significant decreases (p<0.05) in all tissues of fish after exposure to carbofuran. LPO levels increased significantly (p<0.05) in all tissues except gonads after 5 weeks of exposure to carbofuran. There was a significant (p<0.05) increase in DNA fragmentation percentage in treated fish. Our results provide a clear evidence on the response of C. gariepinus to sublethal doses of carbofuran and allow us to consider catfish as a good bioindicator to reflect the endocrine disrupting impacts of carbofuran, and reflect the potential of this pesticide to cause disturbance in antioxidant defense system as well as metabolism and induction of lipid peroxidation (LPO) and DNA damage in contaminated ecosystems.

Authors+Show Affiliations

Zoology Department, Faculty of Science, New Valley Branch, Assiut University, Assiut, Egypt. Electronic address: Ahmedt1983@yahoo.com.Zoology Department, Faculty of Science, Assiut University, Assiut, Egypt; Biology Department, Faculty of Science, North Jeddah, King Abdulaziz University, Jeddah, Saudi Arabia.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

24836875

Citation

Ibrahim, Ahmed Th A., and Ahmed S A. Harabawy. "Sublethal Toxicity of Carbofuran On the African Catfish Clarias Gariepinus: Hormonal, Enzymatic and Antioxidant Responses." Ecotoxicology and Environmental Safety, vol. 106, 2014, pp. 33-9.
Ibrahim AT, Harabawy AS. Sublethal toxicity of carbofuran on the African catfish Clarias gariepinus: Hormonal, enzymatic and antioxidant responses. Ecotoxicol Environ Saf. 2014;106:33-9.
Ibrahim, A. T., & Harabawy, A. S. (2014). Sublethal toxicity of carbofuran on the African catfish Clarias gariepinus: Hormonal, enzymatic and antioxidant responses. Ecotoxicology and Environmental Safety, 106, 33-9. https://doi.org/10.1016/j.ecoenv.2014.04.032
Ibrahim AT, Harabawy AS. Sublethal Toxicity of Carbofuran On the African Catfish Clarias Gariepinus: Hormonal, Enzymatic and Antioxidant Responses. Ecotoxicol Environ Saf. 2014;106:33-9. PubMed PMID: 24836875.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sublethal toxicity of carbofuran on the African catfish Clarias gariepinus: Hormonal, enzymatic and antioxidant responses. AU - Ibrahim,Ahmed Th A, AU - Harabawy,Ahmed S A, Y1 - 2014/05/14/ PY - 2014/01/03/received PY - 2014/04/20/revised PY - 2014/04/21/accepted PY - 2014/5/20/entrez PY - 2014/5/20/pubmed PY - 2014/8/12/medline KW - Antioxidants KW - Carbofuran KW - Clarias KW - Enzymes KW - Hormones KW - Oxidative stress SP - 33 EP - 9 JF - Ecotoxicology and environmental safety JO - Ecotoxicol Environ Saf VL - 106 N2 - The present study examined the impacts of carbofuran on endocrinology of the catfish, Clarias gariepinus, for the first time and evaluated cortisol (CRT), triiodothyronine (T3), thyroxin (T4), 17β-estradiol (E2) and testosterone (TST) and the oxidative stress markers including SOD, CAT, GSTs, GSH. The toxic effects on the metabolic enzymes, G6PDH and LDH, in addition to lipid peroxidation (LPO) and DNA damage as biomarkers in Nile catfish, to sublethal exposures of carbofuran (0.16 and 0.49mg/L, for 35 days) were studied. Statistically significant differences between selected parameters between control and carbofuran-treated fish were recorded. Carbofuran caused a significant (p<0.05) increase in CRT and T3 levels; the mean levels of T4, TST, E2 exhibited significant decreases (p<0.05) in carbofuran-treated fish. Toxicity of carbofuran on liver, kidney, gills, gonads and muscles after 35 days of exposure was found. Glycogen levels showed a highly significant decrease in liver and gills (p< 0.001), a significant decrease (p< 0.05) in kidney and muscles, and insignificant changes (p>0.05) in gonads of treated fish. The two metabolic enzymes G6PDH and LDH in all tissues exhibited significant decreases (p<0.05) in treated fish. SOD, CAT, GSH and GST levels showed significant decreases (p<0.05) in all tissues of fish after exposure to carbofuran. LPO levels increased significantly (p<0.05) in all tissues except gonads after 5 weeks of exposure to carbofuran. There was a significant (p<0.05) increase in DNA fragmentation percentage in treated fish. Our results provide a clear evidence on the response of C. gariepinus to sublethal doses of carbofuran and allow us to consider catfish as a good bioindicator to reflect the endocrine disrupting impacts of carbofuran, and reflect the potential of this pesticide to cause disturbance in antioxidant defense system as well as metabolism and induction of lipid peroxidation (LPO) and DNA damage in contaminated ecosystems. SN - 1090-2414 UR - https://www.unboundmedicine.com/medline/citation/24836875/Sublethal_toxicity_of_carbofuran_on_the_African_catfish_Clarias_gariepinus:_Hormonal_enzymatic_and_antioxidant_responses_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0147-6513(14)00183-3 DB - PRIME DP - Unbound Medicine ER -