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Identification of Angelica oil as a suppressor for the biological properties of Danggui Buxue Tang: a Chinese herbal decoction composes of Astragali Radix and Angelica Sinensis Radix.
J Ethnopharmacol. 2014 Jul 03; 154(3):825-31.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Danggui Buxue Tang (DBT), a Chinese herbal decoction commonly used in treating women׳s ailments, contains two herbs: Angelica Sinensis Radix (ASR) and Astragali Radix (AR). Traditionally, ASR had to be pre-treated with yellow wine before the herbal preparation, which reduced the amount of volatile oil in water extract of ASR and DBT, and meanwhile the volatile oil-reduced DBT processed better bioactivities in cell cultures. The present study aimed to investigate the effect of volatile oil from ASR (Angelica oil) on the solubility of AR-derived ingredients and the biological properties of DBT.

MATERIALS AND METHODS

To standardize Angelica oil, four marker chemicals in ASR were determined by GC-QQQ-MS/MS. Subsequently, fifteen gram of AR was boiled with different amounts of Angelica oil. The amounts of astragaloside IV, calycosin, formononetin, total polysaccharides, total saponins and total flavonoids, all derived from AR, were extracted and determined by HPLC-UV/ELSD. To reveal the effect of Angelica oil on DBT functions, several cell assays related to the traditional functions of DBT were selected, including anti-platelet aggregation, induction of NO production, hematopoetic, estrogenic and osteogenic properties.

RESULTS

The inclusion of Angelica oil in AR during preparation significantly decreased the amount of AR-derived astragaloside IV, calycosin, formononetin, total saponins and total flavonoids in the final water extract. In parallel, an inclusion of Angelica oil caused a decrease of DBT׳s estrogenic and hematopoetic activities in cultured cells. Moreover, the Angelica oil decreased DBT-induced cell proliferation of cultured MG-63 and endothelial cells.

CONCLUSIONS

The results indicated that Angelica oil was a negative regulator for DBT chemically and biologically, which supported the traditional practice of preparing DBT by using the wine-treated ASR.

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Publisher Full Text (DOI)

Authors+Show Affiliations

School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China; Division of Life Science and Center for Chinese Medicine, The Hong Kong University of Science and Technology, Clear Water Bay Road, Hong Kong SAR, China.

Division of Life Science and Center for Chinese Medicine, The Hong Kong University of Science and Technology, Clear Water Bay Road, Hong Kong SAR, China; Department of Biology, Hanshan Normal University, Chaozhou, Guangdong, China.

Division of Life Science and Center for Chinese Medicine, The Hong Kong University of Science and Technology, Clear Water Bay Road, Hong Kong SAR, China.

Division of Life Science and Center for Chinese Medicine, The Hong Kong University of Science and Technology, Clear Water Bay Road, Hong Kong SAR, China.

Division of Life Science and Center for Chinese Medicine, The Hong Kong University of Science and Technology, Clear Water Bay Road, Hong Kong SAR, China.

Division of Life Science and Center for Chinese Medicine, The Hong Kong University of Science and Technology, Clear Water Bay Road, Hong Kong SAR, China.

Division of Life Science and Center for Chinese Medicine, The Hong Kong University of Science and Technology, Clear Water Bay Road, Hong Kong SAR, China.

Division of Life Science and Center for Chinese Medicine, The Hong Kong University of Science and Technology, Clear Water Bay Road, Hong Kong SAR, China. Electronic address: BOTSIM@UST.HK.

MeSH

AngelicaAngelica sinensisAntineoplastic Agents, PhytogenicAstragalus PlantCell ProliferationCell SurvivalDose-Response Relationship, DrugDrug Screening Assays, AntitumorDrugs, Chinese HerbalHEK293 CellsHumansMCF-7 CellsMedicine, Chinese TraditionalMolecular StructureOils, VolatileStructure-Activity RelationshipTumor Cells, Cultured

Pub Type(s)

Journal Article

Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24837305

Citation

Zhan, Janis Ya-Xian, et al. "Identification of Angelica Oil as a Suppressor for the Biological Properties of Danggui Buxue Tang: a Chinese Herbal Decoction Composes of Astragali Radix and Angelica Sinensis Radix." Journal of Ethnopharmacology, vol. 154, no. 3, 2014, pp. 825-31.

Zhan JY, Zheng KY, Zhang WL, et al. Identification of Angelica oil as a suppressor for the biological properties of Danggui Buxue Tang: a Chinese herbal decoction composes of Astragali Radix and Angelica Sinensis Radix. J Ethnopharmacol. 2014;154(3):825-31.

Zhan, J. Y., Zheng, K. Y., Zhang, W. L., Chen, J. P., Yao, P., Bi, C. W., Dong, T. T., & Tsim, K. W. (2014). Identification of Angelica oil as a suppressor for the biological properties of Danggui Buxue Tang: a Chinese herbal decoction composes of Astragali Radix and Angelica Sinensis Radix. Journal of Ethnopharmacology, 154(3), 825-31. https://doi.org/10.1016/j.jep.2014.05.007

Zhan JY, et al. Identification of Angelica Oil as a Suppressor for the Biological Properties of Danggui Buxue Tang: a Chinese Herbal Decoction Composes of Astragali Radix and Angelica Sinensis Radix. J Ethnopharmacol. 2014 Jul 3;154(3):825-31. PubMed PMID: 24837305.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Identification of Angelica oil as a suppressor for the biological properties of Danggui Buxue Tang: a Chinese herbal decoction composes of Astragali Radix and Angelica Sinensis Radix. AU - Zhan,Janis Ya-Xian, AU - Zheng,Ken Yu-Zhong, AU - Zhang,Wendy Li, AU - Chen,Jian-Ping, AU - Yao,Ping, AU - Bi,Cathy Wen-Chuan, AU - Dong,Tina Ting-Xia, AU - Tsim,Karl Wah-Keung, Y1 - 2014/05/14/ PY - 2013/10/11/received PY - 2014/05/05/revised PY - 2014/05/06/accepted PY - 2014/5/20/entrez PY - 2014/5/20/pubmed PY - 2016/5/10/medline KW - Angelica oil KW - Angelica sinensis KW - Astragalus membranaceus var. mongholicus KW - Leguminosae KW - Umbelliferae SP - 825 EP - 31 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 154 IS - 3 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Danggui Buxue Tang (DBT), a Chinese herbal decoction commonly used in treating women׳s ailments, contains two herbs: Angelica Sinensis Radix (ASR) and Astragali Radix (AR). Traditionally, ASR had to be pre-treated with yellow wine before the herbal preparation, which reduced the amount of volatile oil in water extract of ASR and DBT, and meanwhile the volatile oil-reduced DBT processed better bioactivities in cell cultures. The present study aimed to investigate the effect of volatile oil from ASR (Angelica oil) on the solubility of AR-derived ingredients and the biological properties of DBT. MATERIALS AND METHODS: To standardize Angelica oil, four marker chemicals in ASR were determined by GC-QQQ-MS/MS. Subsequently, fifteen gram of AR was boiled with different amounts of Angelica oil. The amounts of astragaloside IV, calycosin, formononetin, total polysaccharides, total saponins and total flavonoids, all derived from AR, were extracted and determined by HPLC-UV/ELSD. To reveal the effect of Angelica oil on DBT functions, several cell assays related to the traditional functions of DBT were selected, including anti-platelet aggregation, induction of NO production, hematopoetic, estrogenic and osteogenic properties. RESULTS: The inclusion of Angelica oil in AR during preparation significantly decreased the amount of AR-derived astragaloside IV, calycosin, formononetin, total saponins and total flavonoids in the final water extract. In parallel, an inclusion of Angelica oil caused a decrease of DBT׳s estrogenic and hematopoetic activities in cultured cells. Moreover, the Angelica oil decreased DBT-induced cell proliferation of cultured MG-63 and endothelial cells. CONCLUSIONS: The results indicated that Angelica oil was a negative regulator for DBT chemically and biologically, which supported the traditional practice of preparing DBT by using the wine-treated ASR. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/24837305/Identification_of_Angelica_oil_as_a_suppressor_for_the_biological_properties_of_Danggui_Buxue_Tang:_a_Chinese_herbal_decoction_composes_of_Astragali_Radix_and_Angelica_Sinensis_Radix_ DB - PRIME DP - Unbound Medicine ER -