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Polyethylene glycol modified magnetic carbon nanotubes as nanosorbents for the determination of methylprednisolone in rat plasma by high performance liquid chromatography.
J Chromatogr A. 2014 Jun 27; 1348:27-33.JC

Abstract

In this paper, polyethylene glycol modified (PEGylated) magnetic carbon nanotubes were developed as solid-phase extraction nanosorbents for the determination of methylprednisolone in rat plasma. The procedure mainly involved two steps including preparation of PEGylated magnetic nanosorbents and bioanalysis. Monodisperse magnetites (Fe3O4) anchored onto multi-walled carbon nanotubes (MWCNTs) were synthesized by a facile solvothermal synthesis method. The obtained MWCNTs-Fe3O4 nanomaterials were further non-covalently functionalized by a surfactant phospholipids-polyethylene glycol (DSPE-PEG). Owing to dispersibility and high enrichment ability, water-soluble PEGylated MWCNTs-Fe3O4 nanomaterials can provide more efficient way for the extraction of methylprednisolone than only MWCNTs-Fe3O4 used. The methylprednisolone could be easily extracted via π-π stacking interactions with PEGylated MWCNTs-Fe3O4. The captured methylprednisolone/nanosorbents were isolated from the matrix by placing a magnet, and desorbed by the elution solvent composed of acetonitrile. Extraction conditions such as amount of nanosorbents added, adsorption time, desorption solvent, and desorption time were investigated and optimized. The method recoveries were obtained from 88.2% to 92.9%. Limits of quantification and limits of detection of 0.01 and 0.005μg/mL were acquired, respectively. The precision ranged from 4.2% to 7.8% for within-day measurement, and for between-day variation was in the range of 5.5-9.0%. Moreover, the analytical performance obtained by PEGylated magnetic MWCNTs was compared with that of magnetic MWCNTs. The results indicated that the approach based on PEGylated magnetic MWCNTs was useful for the analysis of methylprednisolone in the complex plasma.

Authors+Show Affiliations

Department of Orthopaedics, Chinese Air Force General Hospital, No. 30 Fucheng Road, Beijing 100142, China.Department of Neurosurgery, Chinese Air Force General Hospital, No. 30 Fucheng Road, Beijing 100142, China.Department of Orthopaedics, Chinese Air Force General Hospital, No. 30 Fucheng Road, Beijing 100142, China.Department of Orthopaedics, Chinese Air Force General Hospital, No. 30 Fucheng Road, Beijing 100142, China.School of Pharmacy, Fudan University, No. 826 Zhangheng Road, Shanghai 201203, China. Electronic address: sshen@fudan.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

24837418

Citation

Yu, Panfeng, et al. "Polyethylene Glycol Modified Magnetic Carbon Nanotubes as Nanosorbents for the Determination of Methylprednisolone in Rat Plasma By High Performance Liquid Chromatography." Journal of Chromatography. A, vol. 1348, 2014, pp. 27-33.
Yu P, Ma H, Shang Y, et al. Polyethylene glycol modified magnetic carbon nanotubes as nanosorbents for the determination of methylprednisolone in rat plasma by high performance liquid chromatography. J Chromatogr A. 2014;1348:27-33.
Yu, P., Ma, H., Shang, Y., Wu, J., & Shen, S. (2014). Polyethylene glycol modified magnetic carbon nanotubes as nanosorbents for the determination of methylprednisolone in rat plasma by high performance liquid chromatography. Journal of Chromatography. A, 1348, 27-33. https://doi.org/10.1016/j.chroma.2014.04.065
Yu P, et al. Polyethylene Glycol Modified Magnetic Carbon Nanotubes as Nanosorbents for the Determination of Methylprednisolone in Rat Plasma By High Performance Liquid Chromatography. J Chromatogr A. 2014 Jun 27;1348:27-33. PubMed PMID: 24837418.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Polyethylene glycol modified magnetic carbon nanotubes as nanosorbents for the determination of methylprednisolone in rat plasma by high performance liquid chromatography. AU - Yu,Panfeng, AU - Ma,Hongwei, AU - Shang,Yong, AU - Wu,Ji, AU - Shen,Shun, Y1 - 2014/04/28/ PY - 2014/02/02/received PY - 2014/04/18/revised PY - 2014/04/21/accepted PY - 2014/5/20/entrez PY - 2014/5/20/pubmed PY - 2014/8/20/medline KW - Methylprednisolone KW - Nanosorbents KW - PEGylated magnetic carbon nanotubes KW - Rat plasma SP - 27 EP - 33 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1348 N2 - In this paper, polyethylene glycol modified (PEGylated) magnetic carbon nanotubes were developed as solid-phase extraction nanosorbents for the determination of methylprednisolone in rat plasma. The procedure mainly involved two steps including preparation of PEGylated magnetic nanosorbents and bioanalysis. Monodisperse magnetites (Fe3O4) anchored onto multi-walled carbon nanotubes (MWCNTs) were synthesized by a facile solvothermal synthesis method. The obtained MWCNTs-Fe3O4 nanomaterials were further non-covalently functionalized by a surfactant phospholipids-polyethylene glycol (DSPE-PEG). Owing to dispersibility and high enrichment ability, water-soluble PEGylated MWCNTs-Fe3O4 nanomaterials can provide more efficient way for the extraction of methylprednisolone than only MWCNTs-Fe3O4 used. The methylprednisolone could be easily extracted via π-π stacking interactions with PEGylated MWCNTs-Fe3O4. The captured methylprednisolone/nanosorbents were isolated from the matrix by placing a magnet, and desorbed by the elution solvent composed of acetonitrile. Extraction conditions such as amount of nanosorbents added, adsorption time, desorption solvent, and desorption time were investigated and optimized. The method recoveries were obtained from 88.2% to 92.9%. Limits of quantification and limits of detection of 0.01 and 0.005μg/mL were acquired, respectively. The precision ranged from 4.2% to 7.8% for within-day measurement, and for between-day variation was in the range of 5.5-9.0%. Moreover, the analytical performance obtained by PEGylated magnetic MWCNTs was compared with that of magnetic MWCNTs. The results indicated that the approach based on PEGylated magnetic MWCNTs was useful for the analysis of methylprednisolone in the complex plasma. SN - 1873-3778 UR - https://www.unboundmedicine.com/medline/citation/24837418/Polyethylene_glycol_modified_magnetic_carbon_nanotubes_as_nanosorbents_for_the_determination_of_methylprednisolone_in_rat_plasma_by_high_performance_liquid_chromatography_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(14)00655-4 DB - PRIME DP - Unbound Medicine ER -