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Photorhabdus luminescens PirAB-fusion protein exhibits both cytotoxicity and insecticidal activity.
FEMS Microbiol Lett. 2014 Jul; 356(1):23-31.FM

Abstract

The binary toxin 'Photorhabdus insect-related' proteins (PirAB) produced by Photorhabdus luminescens have been reported to possess both injectable and oral activities against a range of insects. Here, PirAB-fusion protein was constructed by linking pirA and pirB genes with the flexible linker (Gly4 Ser)3 DNA encoding sequence and then efficiently expressed in Escherichia coli. To better understand the role of PirAB toxin played in the process of invasion, its cytotoxicity against insect midgut CF-203 cells was investigated. Application of purified PirAB-fusion protein as well as PirA/PirB mixture caused loss of viability of CF-203 cells after 24 h incubation. CF-203 cells treated by PirAB-fusion protein displayed morphological changes typical of apoptosis, such as cell shrinkage, cell membrane blebbing, nuclear condensation and DNA fragmentation. Moreover, PirAB-fusion protein also exhibited injectable insecticidal activity against Spodoptera exigua larvae. The bodies of S. exigua fourth-instar larvae injected with PirAB-fusion protein turned completely black. Thus, we concluded that PirAB-fusion protein possessed similar biological activity (cytotoxicity and insecticidal activity) to PirA/PirB mixture, which would enable it to be used as an efficient agent for pest control.

Authors+Show Affiliations

College of Life Sciences, Hunan Provincial Key Laboratory of Microbial Molecular Biology - State Key Laboratory Breeding Base of Microbial Molecular Biology, Hunan Normal University, Changsha, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24840022

Citation

Li, Yusheng, et al. "Photorhabdus Luminescens PirAB-fusion Protein Exhibits Both Cytotoxicity and Insecticidal Activity." FEMS Microbiology Letters, vol. 356, no. 1, 2014, pp. 23-31.
Li Y, Hu X, Zhang X, et al. Photorhabdus luminescens PirAB-fusion protein exhibits both cytotoxicity and insecticidal activity. FEMS Microbiol Lett. 2014;356(1):23-31.
Li, Y., Hu, X., Zhang, X., Liu, Z., Ding, X., Xia, L., & Hu, S. (2014). Photorhabdus luminescens PirAB-fusion protein exhibits both cytotoxicity and insecticidal activity. FEMS Microbiology Letters, 356(1), 23-31. https://doi.org/10.1111/1574-6968.12474
Li Y, et al. Photorhabdus Luminescens PirAB-fusion Protein Exhibits Both Cytotoxicity and Insecticidal Activity. FEMS Microbiol Lett. 2014;356(1):23-31. PubMed PMID: 24840022.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Photorhabdus luminescens PirAB-fusion protein exhibits both cytotoxicity and insecticidal activity. AU - Li,Yusheng, AU - Hu,Xiaofeng, AU - Zhang,Xu, AU - Liu,Zhengqiang, AU - Ding,Xuezhi, AU - Xia,Liqiu, AU - Hu,Shengbiao, Y1 - 2014/06/16/ PY - 2014/04/03/received PY - 2014/04/29/revised PY - 2014/05/07/accepted PY - 2014/5/21/entrez PY - 2014/5/21/pubmed PY - 2015/3/31/medline KW - Photorhabdus luminescens KW - PirAB KW - apoptosis KW - cytotoxicity KW - fusion protein KW - injectable activity SP - 23 EP - 31 JF - FEMS microbiology letters JO - FEMS Microbiol Lett VL - 356 IS - 1 N2 - The binary toxin 'Photorhabdus insect-related' proteins (PirAB) produced by Photorhabdus luminescens have been reported to possess both injectable and oral activities against a range of insects. Here, PirAB-fusion protein was constructed by linking pirA and pirB genes with the flexible linker (Gly4 Ser)3 DNA encoding sequence and then efficiently expressed in Escherichia coli. To better understand the role of PirAB toxin played in the process of invasion, its cytotoxicity against insect midgut CF-203 cells was investigated. Application of purified PirAB-fusion protein as well as PirA/PirB mixture caused loss of viability of CF-203 cells after 24 h incubation. CF-203 cells treated by PirAB-fusion protein displayed morphological changes typical of apoptosis, such as cell shrinkage, cell membrane blebbing, nuclear condensation and DNA fragmentation. Moreover, PirAB-fusion protein also exhibited injectable insecticidal activity against Spodoptera exigua larvae. The bodies of S. exigua fourth-instar larvae injected with PirAB-fusion protein turned completely black. Thus, we concluded that PirAB-fusion protein possessed similar biological activity (cytotoxicity and insecticidal activity) to PirA/PirB mixture, which would enable it to be used as an efficient agent for pest control. SN - 1574-6968 UR - https://www.unboundmedicine.com/medline/citation/24840022/Photorhabdus_luminescens_PirAB_fusion_protein_exhibits_both_cytotoxicity_and_insecticidal_activity_ DB - PRIME DP - Unbound Medicine ER -