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Streptococcus pneumoniae Interacts with pIgR expressed by the brain microvascular endothelium but does not co-localize with PAF receptor.
PLoS One. 2014; 9(5):e97914.Plos

Abstract

Streptococcus pneumoniae is thought to adhere to the blood-brain barrier (BBB) endothelium prior to causing meningitis. The platelet activating factor receptor (PAFR) has been implicated in this adhesion but there is a paucity of data demonstrating direct binding of the bacteria to PAFR. Additionally, studies that inhibit PAFR strongly suggest that alternative receptors for pneumococci are present on the endothelium. Therefore, we studied the roles of PAFR and pIgR, an established epithelial pneumococcal receptor, in pneumococcal adhesion to brain endothelial cells in vivo. Mice were intravenously infected with pneumococci and sacrificed at various time points before meningitis onset. Co-localization of bacteria with PAFR and pIgR was investigated using immunofluorescent analysis of the brain tissue. In vitro blocking with antibodies and incubation of pneumococci with endothelial cell lysates were used to further probe bacteria-receptor interaction. In vivo as well as in vitro pneumococci did not co-localize with PAFR. On the other hand the majority of S. pneumoniae co-localized with endothelial pIgR and pIgR blocking reduced pneumococcal adhesion to endothelial cells. Pneumococci physically interacted with pIgR in endothelial cell lysates. In conclusion, bacteria did not associate with PAFR, indicating an indirect role of PAFR in pneumococcal adhesion to endothelial cells. In contrast, pIgR on the BBB endothelium may represent a novel pneumococcal adhesion receptor.

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Authors+Show Affiliations

Iovino F
Department of Medical Microbiology, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands.
Molema G
Department of Pathology & Medical Biology, Medical Biology section, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands.
Bijlsma JJ
Department of Medical Microbiology, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands.

MeSH

AnimalsBacterial AdhesionBlood-Brain BarrierBlotting, WesternCell LineFluorescent Antibody TechniqueHost-Pathogen InteractionsHumansImage Processing, Computer-AssistedMicePlatelet Membrane GlycoproteinsReceptors, G-Protein-CoupledReceptors, Polymeric ImmunoglobulinStreptococcus pneumoniae

Pub Type(s)

Journal Article

Language

eng

PubMed ID

24841255

Citation

Iovino, Federico, et al. "Streptococcus Pneumoniae Interacts With pIgR Expressed By the Brain Microvascular Endothelium but Does Not Co-localize With PAF Receptor." PloS One, vol. 9, no. 5, 2014, pp. e97914.
Iovino F, Molema G, Bijlsma JJ. Streptococcus pneumoniae Interacts with pIgR expressed by the brain microvascular endothelium but does not co-localize with PAF receptor. PLoS One. 2014;9(5):e97914.
Iovino, F., Molema, G., & Bijlsma, J. J. (2014). Streptococcus pneumoniae Interacts with pIgR expressed by the brain microvascular endothelium but does not co-localize with PAF receptor. PloS One, 9(5), e97914. https://doi.org/10.1371/journal.pone.0097914
Iovino F, Molema G, Bijlsma JJ. Streptococcus Pneumoniae Interacts With pIgR Expressed By the Brain Microvascular Endothelium but Does Not Co-localize With PAF Receptor. PLoS One. 2014;9(5):e97914. PubMed PMID: 24841255.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Streptococcus pneumoniae Interacts with pIgR expressed by the brain microvascular endothelium but does not co-localize with PAF receptor. AU - Iovino,Federico, AU - Molema,Grietje, AU - Bijlsma,Jetta J E, Y1 - 2014/05/19/ PY - 2013/10/29/received PY - 2014/04/26/accepted PY - 2014/5/21/entrez PY - 2014/5/21/pubmed PY - 2015/1/13/medline SP - e97914 EP - e97914 JF - PloS one JO - PLoS One VL - 9 IS - 5 N2 - Streptococcus pneumoniae is thought to adhere to the blood-brain barrier (BBB) endothelium prior to causing meningitis. The platelet activating factor receptor (PAFR) has been implicated in this adhesion but there is a paucity of data demonstrating direct binding of the bacteria to PAFR. Additionally, studies that inhibit PAFR strongly suggest that alternative receptors for pneumococci are present on the endothelium. Therefore, we studied the roles of PAFR and pIgR, an established epithelial pneumococcal receptor, in pneumococcal adhesion to brain endothelial cells in vivo. Mice were intravenously infected with pneumococci and sacrificed at various time points before meningitis onset. Co-localization of bacteria with PAFR and pIgR was investigated using immunofluorescent analysis of the brain tissue. In vitro blocking with antibodies and incubation of pneumococci with endothelial cell lysates were used to further probe bacteria-receptor interaction. In vivo as well as in vitro pneumococci did not co-localize with PAFR. On the other hand the majority of S. pneumoniae co-localized with endothelial pIgR and pIgR blocking reduced pneumococcal adhesion to endothelial cells. Pneumococci physically interacted with pIgR in endothelial cell lysates. In conclusion, bacteria did not associate with PAFR, indicating an indirect role of PAFR in pneumococcal adhesion to endothelial cells. In contrast, pIgR on the BBB endothelium may represent a novel pneumococcal adhesion receptor. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/24841255/Streptococcus_pneumoniae_Interacts_with_pIgR_expressed_by_the_brain_microvascular_endothelium_but_does_not_co_localize_with_PAF_receptor_ DB - PRIME DP - Unbound Medicine ER -