Tags

Type your tag names separated by a space and hit enter

D-amino acids enhance the activity of antimicrobials against biofilms of clinical wound isolates of Staphylococcus aureus and Pseudomonas aeruginosa.
Antimicrob Agents Chemother. 2014 Aug; 58(8):4353-61.AA

Abstract

Within wounds, microorganisms predominantly exist as biofilms. Biofilms are associated with chronic infections and represent a tremendous clinical challenge. As antibiotics are often ineffective against biofilms, use of dispersal agents as adjunctive, topical therapies for the treatment of wound infections involving biofilms has gained interest. We evaluated in vitro the dispersive activity of D-amino acids (D-AAs) on biofilms from clinical wound isolates of Staphylococcus aureus and Pseudomonas aeruginosa; moreover, we determined whether combinations of D-AAs and antibiotics (clindamycin, cefazolin, oxacillin, rifampin, and vancomycin for S. aureus and amikacin, colistin, ciprofloxacin, imipenem, and ceftazidime for P. aeruginosa) enhance activity against biofilms. D-Met, D-Phe, and D-Trp at concentrations of ≥ 5 mM effectively dispersed preformed biofilms of S. aureus and P. aeruginosa clinical isolates, an effect that was enhanced when they were combined as an equimolar mixture (D-Met/D-Phe/D-Trp). When combined with D-AAs, the activity of rifampin was significantly enhanced against biofilms of clinical isolates of S. aureus, as indicated by a reduction in the minimum biofilm inhibitory concentration (MBIC) (from 32 to 8 μg/ml) and a >2-log reduction of viable biofilm bacteria compared to treatment with antibiotic alone. The addition of D-AAs was also observed to enhance the activity of colistin and ciprofloxacin against biofilms of P. aeruginosa, reducing the observed MBIC and the number of viable bacteria by >2 logs and 1 log at 64 and 32 μg/ml in contrast to antibiotics alone. These findings indicate that the biofilm dispersal activity of D-AAs may represent an effective strategy, in combination with antimicrobials, to release bacteria from biofilms, subsequently enhancing antimicrobial activity.

Authors+Show Affiliations

United States Army Institute of Surgical Research, Extremity Trauma and Regenerative Medicine, Fort Sam Houston, Texas, USA carlos.j.sanchez47.vol@mail.mil.United States Army Institute of Surgical Research, Extremity Trauma and Regenerative Medicine, Fort Sam Houston, Texas, USA Infectious Disease Service, Department of Medicine, San Antonio Military Medical Center, Fort Sam Houston, San Antonio, Texas, USA.United States Army Institute of Surgical Research, Extremity Trauma and Regenerative Medicine, Fort Sam Houston, Texas, USA.United States Army Institute of Surgical Research, Extremity Trauma and Regenerative Medicine, Fort Sam Houston, Texas, USA.United States Army Institute of Surgical Research, Extremity Trauma and Regenerative Medicine, Fort Sam Houston, Texas, USA.Infectious Disease Service, Department of Medicine, San Antonio Military Medical Center, Fort Sam Houston, San Antonio, Texas, USA.United States Army Institute of Surgical Research, Extremity Trauma and Regenerative Medicine, Fort Sam Houston, Texas, USA.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

24841260

Citation

Sanchez, Carlos J., et al. "D-amino Acids Enhance the Activity of Antimicrobials Against Biofilms of Clinical Wound Isolates of Staphylococcus Aureus and Pseudomonas Aeruginosa." Antimicrobial Agents and Chemotherapy, vol. 58, no. 8, 2014, pp. 4353-61.
Sanchez CJ, Akers KS, Romano DR, et al. D-amino acids enhance the activity of antimicrobials against biofilms of clinical wound isolates of Staphylococcus aureus and Pseudomonas aeruginosa. Antimicrob Agents Chemother. 2014;58(8):4353-61.
Sanchez, C. J., Akers, K. S., Romano, D. R., Woodbury, R. L., Hardy, S. K., Murray, C. K., & Wenke, J. C. (2014). D-amino acids enhance the activity of antimicrobials against biofilms of clinical wound isolates of Staphylococcus aureus and Pseudomonas aeruginosa. Antimicrobial Agents and Chemotherapy, 58(8), 4353-61. https://doi.org/10.1128/AAC.02468-14
Sanchez CJ, et al. D-amino Acids Enhance the Activity of Antimicrobials Against Biofilms of Clinical Wound Isolates of Staphylococcus Aureus and Pseudomonas Aeruginosa. Antimicrob Agents Chemother. 2014;58(8):4353-61. PubMed PMID: 24841260.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - D-amino acids enhance the activity of antimicrobials against biofilms of clinical wound isolates of Staphylococcus aureus and Pseudomonas aeruginosa. AU - Sanchez,Carlos J,Jr AU - Akers,Kevin S, AU - Romano,Desiree R, AU - Woodbury,Ronald L, AU - Hardy,Sharanda K, AU - Murray,Clinton K, AU - Wenke,Joseph C, Y1 - 2014/05/19/ PY - 2014/5/21/entrez PY - 2014/5/21/pubmed PY - 2015/9/5/medline SP - 4353 EP - 61 JF - Antimicrobial agents and chemotherapy JO - Antimicrob. Agents Chemother. VL - 58 IS - 8 N2 - Within wounds, microorganisms predominantly exist as biofilms. Biofilms are associated with chronic infections and represent a tremendous clinical challenge. As antibiotics are often ineffective against biofilms, use of dispersal agents as adjunctive, topical therapies for the treatment of wound infections involving biofilms has gained interest. We evaluated in vitro the dispersive activity of D-amino acids (D-AAs) on biofilms from clinical wound isolates of Staphylococcus aureus and Pseudomonas aeruginosa; moreover, we determined whether combinations of D-AAs and antibiotics (clindamycin, cefazolin, oxacillin, rifampin, and vancomycin for S. aureus and amikacin, colistin, ciprofloxacin, imipenem, and ceftazidime for P. aeruginosa) enhance activity against biofilms. D-Met, D-Phe, and D-Trp at concentrations of ≥ 5 mM effectively dispersed preformed biofilms of S. aureus and P. aeruginosa clinical isolates, an effect that was enhanced when they were combined as an equimolar mixture (D-Met/D-Phe/D-Trp). When combined with D-AAs, the activity of rifampin was significantly enhanced against biofilms of clinical isolates of S. aureus, as indicated by a reduction in the minimum biofilm inhibitory concentration (MBIC) (from 32 to 8 μg/ml) and a >2-log reduction of viable biofilm bacteria compared to treatment with antibiotic alone. The addition of D-AAs was also observed to enhance the activity of colistin and ciprofloxacin against biofilms of P. aeruginosa, reducing the observed MBIC and the number of viable bacteria by >2 logs and 1 log at 64 and 32 μg/ml in contrast to antibiotics alone. These findings indicate that the biofilm dispersal activity of D-AAs may represent an effective strategy, in combination with antimicrobials, to release bacteria from biofilms, subsequently enhancing antimicrobial activity. SN - 1098-6596 UR - https://www.unboundmedicine.com/medline/citation/24841260/D_amino_acids_enhance_the_activity_of_antimicrobials_against_biofilms_of_clinical_wound_isolates_of_Staphylococcus_aureus_and_Pseudomonas_aeruginosa_ L2 - http://aac.asm.org/cgi/pmidlookup?view=long&pmid=24841260 DB - PRIME DP - Unbound Medicine ER -