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Cross-amplification of nonspecific microsatellites markers: a useful tool to study endangered/vulnerable species of southern Andes deer.
Genet Mol Res 2014; 13(2):3193-200GM

Abstract

Thirty-nine microsatellite loci that are highly conserved in red deer, sika deer, reindeer, Soay sheep, and other artiodactyls were tested in two vulnerable and endangered Neotropical deer (pudu: Pudu puda and huemul: Hippocamelus bisulcus) with the aim of producing a standardized set of markers that can be used successfully in noninvasive samples from these species. We also compared these nonspecific loci against eight polymorphic loci that were recently developed for huemul to determine whether the nonspecific markers could reflect the huemul's genetic variation that was observed with the specific loci. We identified 10 suitable loci, six of which constitute a standardized set for the two species and can be used to identify them in the absence of phenotypic data. The expected heterozygosity per locus for the panel of six loci ranged from 0.461 to 0.889 (average 0.665), and the maximum probability of identity value was 6.9x10(-6) and 3.2x10(-4) in pudu and huemul, respectively. This set of loci has potential applications in evolutionary, ecological, forensic, and conservation studies in pudu and huemul.

Authors+Show Affiliations

Laboratory of Genomic and Biodiversity, Department of Basic Sciences, University of Bío-Bío, Chillán, Chile jcmarin@ubiobio.cl.School of Biosciences, Cardiff University, Cardiff, Wales, UK.Laboratory of Genomic and Biodiversity, Department of Basic Sciences, University of Bío-Bío, Chillán, Chile.Laboratory of Genomic and Biodiversity, Department of Basic Sciences, University of Bío-Bío, Chillán, Chile.Institute of Environmental Science and Evolution, Faculty of Sciences, Austral University of Chile, Valdivia, Chile.Departament of Ecosystem and Environment, Pontifical Catholic University of Chile, Santiago, Chile.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24841651

Citation

Marín, J C., et al. "Cross-amplification of Nonspecific Microsatellites Markers: a Useful Tool to Study Endangered/vulnerable Species of Southern Andes Deer." Genetics and Molecular Research : GMR, vol. 13, no. 2, 2014, pp. 3193-200.
Marín JC, Orozco-terWengel P, Romero K, et al. Cross-amplification of nonspecific microsatellites markers: a useful tool to study endangered/vulnerable species of southern Andes deer. Genet Mol Res. 2014;13(2):3193-200.
Marín, J. C., Orozco-terWengel, P., Romero, K., Vásquez, J. P., Varas, V., & Vianna, J. A. (2014). Cross-amplification of nonspecific microsatellites markers: a useful tool to study endangered/vulnerable species of southern Andes deer. Genetics and Molecular Research : GMR, 13(2), pp. 3193-200. doi:10.4238/2014.April.25.4.
Marín JC, et al. Cross-amplification of Nonspecific Microsatellites Markers: a Useful Tool to Study Endangered/vulnerable Species of Southern Andes Deer. Genet Mol Res. 2014 Apr 25;13(2):3193-200. PubMed PMID: 24841651.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cross-amplification of nonspecific microsatellites markers: a useful tool to study endangered/vulnerable species of southern Andes deer. AU - Marín,J C, AU - Orozco-terWengel,P, AU - Romero,K, AU - Vásquez,J P, AU - Varas,V, AU - Vianna,J A, Y1 - 2014/04/25/ PY - 2014/5/21/entrez PY - 2014/5/21/pubmed PY - 2014/12/15/medline SP - 3193 EP - 200 JF - Genetics and molecular research : GMR JO - Genet. Mol. Res. VL - 13 IS - 2 N2 - Thirty-nine microsatellite loci that are highly conserved in red deer, sika deer, reindeer, Soay sheep, and other artiodactyls were tested in two vulnerable and endangered Neotropical deer (pudu: Pudu puda and huemul: Hippocamelus bisulcus) with the aim of producing a standardized set of markers that can be used successfully in noninvasive samples from these species. We also compared these nonspecific loci against eight polymorphic loci that were recently developed for huemul to determine whether the nonspecific markers could reflect the huemul's genetic variation that was observed with the specific loci. We identified 10 suitable loci, six of which constitute a standardized set for the two species and can be used to identify them in the absence of phenotypic data. The expected heterozygosity per locus for the panel of six loci ranged from 0.461 to 0.889 (average 0.665), and the maximum probability of identity value was 6.9x10(-6) and 3.2x10(-4) in pudu and huemul, respectively. This set of loci has potential applications in evolutionary, ecological, forensic, and conservation studies in pudu and huemul. SN - 1676-5680 UR - https://www.unboundmedicine.com/medline/citation/24841651/Cross_amplification_of_nonspecific_microsatellites_markers:_a_useful_tool_to_study_endangered/vulnerable_species_of_southern_Andes_deer_ L2 - http://www.geneticsmr.com/articles/3137 DB - PRIME DP - Unbound Medicine ER -