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Polyphenol-rich bilberry ameliorates total cholesterol and LDL-cholesterol when implemented in the diet of Zucker diabetic fatty rats.
Rev Diabet Stud 2013; 10(4):270-82RD

Abstract

BACKGROUND

Bilberries and blackcurrants are nutrient sources rich in bioactive components, including dietary fibers, polyphenols, and anthocyanins, which possess potent cardiovascular protective properties. Few studies investigating the cardio-protective effects of natural components have focused on whole bilberries or blackcurrants.

OBJECTIVE

The aim of this trial was to investigate whether a diet enriched with bilberries or blackcurrants has beneficial effects on glucose metabolism, lipid profile, blood pressure, and expression of genes related to glucose and lipid metabolism.

METHODS

Male Zucker Diabetic Fatty (ZDF) rats (n = 48) were randomly assigned to either a control, bilberry-enriched, blackcurrant-enriched, or fiber-enriched diet for 8 weeks ad libitum. Real-time quantitative PCR analysis was performed on liver, adipose, and muscle tissue. Berry polyphenol content was determined by HPLC and LC-MS analysis.

RESULTS

Bilberry enrichment reduced total (-21%, p = 0.0132) and LDL-cholesterol (-60%, p = 0.0229) levels, but increased HDL-cholesterol to a lesser extent than in controls. This may partly be due to the altered hepatic liver X receptor-α expression (-24%, p < 0.001). Neither bilberries nor blackcurrants influenced glucose metabolism or blood pressure. Nevertheless, transcriptional analysis implied a better conservation of hepatic and adipocyte insulin sensitivity by bilberry enrichment. Anthocyanins constituted 91% and 87% of total polyphenol content in bilberries and blackcurrants, respectively. However, total anthocyanin content (3441 mg/100 g) was 4-fold higher in bilberries than in blackcurrants (871 mg/100 g).

CONCLUSIONS

Bilberry consumption ameliorated total and LDL-cholesterol levels, but not HDL-cholesterol levels in ZDF rats. Neither bilberry nor blackcurrant enrichment delayed the development of diabetes or hypertension. Thus, in rats, bilberries may be valuable as a dietary preventive agent against hypercholesterolemia, probably by virtue of their high anthocyanin content.

Authors+Show Affiliations

Department of Endocrinology and Internal Medicine, Aarhus University Hospital, Aarhus C, Denmark.Department of Endocrinology and Internal Medicine, Aarhus University Hospital, Aarhus C, Denmark.Department of Chemical Engineering, Biotechnology and Environmental Technology, Faculty of Engineering, University of Southern Denmark, Odense, Denmark.Department of Endocrinology and Internal Medicine, Aarhus University Hospital, Aarhus C, Denmark.Department of Endocrinology and Internal Medicine, Aarhus University Hospital, Aarhus C, Denmark.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24841880

Citation

Brader, Lea, et al. "Polyphenol-rich Bilberry Ameliorates Total Cholesterol and LDL-cholesterol when Implemented in the Diet of Zucker Diabetic Fatty Rats." The Review of Diabetic Studies : RDS, vol. 10, no. 4, 2013, pp. 270-82.
Brader L, Overgaard A, Christensen LP, et al. Polyphenol-rich bilberry ameliorates total cholesterol and LDL-cholesterol when implemented in the diet of Zucker diabetic fatty rats. Rev Diabet Stud. 2013;10(4):270-82.
Brader, L., Overgaard, A., Christensen, L. P., Jeppesen, P. B., & Hermansen, K. (2013). Polyphenol-rich bilberry ameliorates total cholesterol and LDL-cholesterol when implemented in the diet of Zucker diabetic fatty rats. The Review of Diabetic Studies : RDS, 10(4), pp. 270-82. doi:10.1900/RDS.2013.10.270.
Brader L, et al. Polyphenol-rich Bilberry Ameliorates Total Cholesterol and LDL-cholesterol when Implemented in the Diet of Zucker Diabetic Fatty Rats. Rev Diabet Stud. 2013;10(4):270-82. PubMed PMID: 24841880.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Polyphenol-rich bilberry ameliorates total cholesterol and LDL-cholesterol when implemented in the diet of Zucker diabetic fatty rats. AU - Brader,Lea, AU - Overgaard,Ann, AU - Christensen,Lars P, AU - Jeppesen,Per B, AU - Hermansen,Kjeld, Y1 - 2014/02/10/ PY - 2014/5/21/entrez PY - 2013/1/1/pubmed PY - 2015/1/13/medline SP - 270 EP - 82 JF - The review of diabetic studies : RDS JO - Rev Diabet Stud VL - 10 IS - 4 N2 - BACKGROUND: Bilberries and blackcurrants are nutrient sources rich in bioactive components, including dietary fibers, polyphenols, and anthocyanins, which possess potent cardiovascular protective properties. Few studies investigating the cardio-protective effects of natural components have focused on whole bilberries or blackcurrants. OBJECTIVE: The aim of this trial was to investigate whether a diet enriched with bilberries or blackcurrants has beneficial effects on glucose metabolism, lipid profile, blood pressure, and expression of genes related to glucose and lipid metabolism. METHODS: Male Zucker Diabetic Fatty (ZDF) rats (n = 48) were randomly assigned to either a control, bilberry-enriched, blackcurrant-enriched, or fiber-enriched diet for 8 weeks ad libitum. Real-time quantitative PCR analysis was performed on liver, adipose, and muscle tissue. Berry polyphenol content was determined by HPLC and LC-MS analysis. RESULTS: Bilberry enrichment reduced total (-21%, p = 0.0132) and LDL-cholesterol (-60%, p = 0.0229) levels, but increased HDL-cholesterol to a lesser extent than in controls. This may partly be due to the altered hepatic liver X receptor-α expression (-24%, p < 0.001). Neither bilberries nor blackcurrants influenced glucose metabolism or blood pressure. Nevertheless, transcriptional analysis implied a better conservation of hepatic and adipocyte insulin sensitivity by bilberry enrichment. Anthocyanins constituted 91% and 87% of total polyphenol content in bilberries and blackcurrants, respectively. However, total anthocyanin content (3441 mg/100 g) was 4-fold higher in bilberries than in blackcurrants (871 mg/100 g). CONCLUSIONS: Bilberry consumption ameliorated total and LDL-cholesterol levels, but not HDL-cholesterol levels in ZDF rats. Neither bilberry nor blackcurrant enrichment delayed the development of diabetes or hypertension. Thus, in rats, bilberries may be valuable as a dietary preventive agent against hypercholesterolemia, probably by virtue of their high anthocyanin content. SN - 1614-0575 UR - https://www.unboundmedicine.com/medline/citation/24841880/Polyphenol_rich_bilberry_ameliorates_total_cholesterol_and_LDL_cholesterol_when_implemented_in_the_diet_of_Zucker_diabetic_fatty_rats_ L2 - http://www.soc-bdr.org/content/e4/articlelookup?showfulltext=1&amp;volume=10&amp;firstpage=270 DB - PRIME DP - Unbound Medicine ER -