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Phenotypic screening and molecular analysis of blast resistance in fragrant rice for marker assisted selection.
C R Biol 2014; 337(5):318-24CR

Abstract

Experiments were conducted to identify blast-resistant fragrant genotypes for the development of a durable blast-resistant rice variety during years 2012-2013. The results indicate that out of 140 test materials including 114 fragrant germplasms, 25 differential varieties (DVs) harbouring 23 blast-resistant genes, only 16 fragrant rice germplasms showed comparatively better performance against a virulent isolate of blast disease. The reaction pattern of single-spore isolate of Magnaporthe oryzae to differential varieties showed that Pish, Pi9, Pita-2 and Pita are the effective blast-resistant genes against the tested blast isolates in Bangladesh. The DNA markers profiles of selected 16 rice germplasms indicated that genotype Chinigura contained Pish, Pi9 and Pita genes; on the other hand, both BRRI dhan50 and Bawaibhog contained Pish and Pita genes in their genetic background. Genotypes Jirakatari, BR5, and Gopalbhog possessed Pish gene, while Uknimodhu, Deshikatari, Radhunipagol, Kalijira (3), Chinikanai each contained the Pita gene only. There are some materials that did not contain any target gene(s) in their genetic background, but proved resistant in pathogenicity tests. This information provided valuable genetic information for breeders to develop durable blast-resistant fragrant or aromatic rice varieties in Bangladesh.

Authors+Show Affiliations

Plant Pathology Division, Bangladesh Rice Research Institute, Gazipur 1701, Bangladesh.Department of Genetics and Plant Breeding, Patuakhali Science and Technology University, Patuakhali 8602, Bangladesh.Plant Pathology Division, Bangladesh Rice Research Institute, Gazipur 1701, Bangladesh.Plant Pathology Division, Bangladesh Rice Research Institute, Gazipur 1701, Bangladesh.Department of Genetics and Plant Breeding, Patuakhali Science and Technology University, Patuakhali 8602, Bangladesh.Japan International Research Center for Agricultural Sciences, 1-1, Ohwashi, Tsukuba, Ibaraki 305-8686, Japan.Plant Pathology Division, Bangladesh Rice Research Institute, Gazipur 1701, Bangladesh.Plant Pathology Division, Bangladesh Rice Research Institute, Gazipur 1701, Bangladesh; Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, 43400 UPM, Serdang, Sealngor, Malaysia. Electronic address: alatif1965@yahoo.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24841958

Citation

Khan, Mohammad Ashik Iqbal, et al. "Phenotypic Screening and Molecular Analysis of Blast Resistance in Fragrant Rice for Marker Assisted Selection." Comptes Rendus Biologies, vol. 337, no. 5, 2014, pp. 318-24.
Khan MA, Sen PP, Bhuiyan R, et al. Phenotypic screening and molecular analysis of blast resistance in fragrant rice for marker assisted selection. C R Biol. 2014;337(5):318-24.
Khan, M. A., Sen, P. P., Bhuiyan, R., Kabir, E., Chowdhury, A. K., Fukuta, Y., ... Latif, M. A. (2014). Phenotypic screening and molecular analysis of blast resistance in fragrant rice for marker assisted selection. Comptes Rendus Biologies, 337(5), pp. 318-24. doi:10.1016/j.crvi.2014.02.007.
Khan MA, et al. Phenotypic Screening and Molecular Analysis of Blast Resistance in Fragrant Rice for Marker Assisted Selection. C R Biol. 2014;337(5):318-24. PubMed PMID: 24841958.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Phenotypic screening and molecular analysis of blast resistance in fragrant rice for marker assisted selection. AU - Khan,Mohammad Ashik Iqbal, AU - Sen,Partha Pratim, AU - Bhuiyan,Rejwan, AU - Kabir,Enamul, AU - Chowdhury,Abul Kashem, AU - Fukuta,Yoshimichi, AU - Ali,Ansar, AU - Latif,Mohammad Abdul, Y1 - 2014/03/24/ PY - 2014/01/06/received PY - 2014/02/20/revised PY - 2014/02/20/accepted PY - 2014/5/21/entrez PY - 2014/5/21/pubmed PY - 2015/1/1/medline KW - Blast disease KW - DNA markers KW - Fragrant rice KW - Pathogenicity KW - Resistance SP - 318 EP - 24 JF - Comptes rendus biologies JO - C. R. Biol. VL - 337 IS - 5 N2 - Experiments were conducted to identify blast-resistant fragrant genotypes for the development of a durable blast-resistant rice variety during years 2012-2013. The results indicate that out of 140 test materials including 114 fragrant germplasms, 25 differential varieties (DVs) harbouring 23 blast-resistant genes, only 16 fragrant rice germplasms showed comparatively better performance against a virulent isolate of blast disease. The reaction pattern of single-spore isolate of Magnaporthe oryzae to differential varieties showed that Pish, Pi9, Pita-2 and Pita are the effective blast-resistant genes against the tested blast isolates in Bangladesh. The DNA markers profiles of selected 16 rice germplasms indicated that genotype Chinigura contained Pish, Pi9 and Pita genes; on the other hand, both BRRI dhan50 and Bawaibhog contained Pish and Pita genes in their genetic background. Genotypes Jirakatari, BR5, and Gopalbhog possessed Pish gene, while Uknimodhu, Deshikatari, Radhunipagol, Kalijira (3), Chinikanai each contained the Pita gene only. There are some materials that did not contain any target gene(s) in their genetic background, but proved resistant in pathogenicity tests. This information provided valuable genetic information for breeders to develop durable blast-resistant fragrant or aromatic rice varieties in Bangladesh. SN - 1768-3238 UR - https://www.unboundmedicine.com/medline/citation/24841958/Phenotypic_screening_and_molecular_analysis_of_blast_resistance_in_fragrant_rice_for_marker_assisted_selection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1631-0691(14)00048-1 DB - PRIME DP - Unbound Medicine ER -