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Simultaneous determination of neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid and geniposide in rat plasma by UPLC-MS/MS and its application to a pharmacokinetic study after administration of Reduning injection.
Biomed Chromatogr. 2015 Jan; 29(1):68-74.BC

Abstract

A simple, specific and sensitive ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method was established and validated for simultaneous determination of neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid and geniposide in rat plasma using puerarin as an internal standard (IS). Plasma samples were pretreated by a one-step direct protein precipitation procedure with acetonitrile after acidified using as little as 50 μL plasma. Chromatographic separation was performed on an Acquity BEH C18 column (100 × 2.1 mm, 1.7 µm) at a flow rate of 0.2 mL/min by a gradient elution, using 0.2% acetic acid-methanol as mobile phase. The detection was performed on a triple quadrupole tandem mass spectrometer by multiple reaction monitoring via electrospray ionization source with negative ion mode. Calibration curves showed good linearity (r > 0.995) over wide concentration ranges. The intra- and inter-day precisions were <15%, and the accuracy was within ±8.0%. The validated method was successfully applied to a pharmacokinetic study of the four bioactive components in rats after intravenous administration of Reduning injection.

Authors+Show Affiliations

School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, 110016, China; State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Processes, 58 Haichang South Road, Xinpu District, Lianyungang, 222001, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24842397

Citation

Wang, Yanjuan, et al. "Simultaneous Determination of Neochlorogenic Acid, Chlorogenic Acid, Cryptochlorogenic Acid and Geniposide in Rat Plasma By UPLC-MS/MS and Its Application to a Pharmacokinetic Study After Administration of Reduning Injection." Biomedical Chromatography : BMC, vol. 29, no. 1, 2015, pp. 68-74.
Wang Y, Wen J, Zheng W, et al. Simultaneous determination of neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid and geniposide in rat plasma by UPLC-MS/MS and its application to a pharmacokinetic study after administration of Reduning injection. Biomed Chromatogr. 2015;29(1):68-74.
Wang, Y., Wen, J., Zheng, W., Zhao, L., Fu, X., Wang, Z., Xiong, Z., Li, F., & Xiao, W. (2015). Simultaneous determination of neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid and geniposide in rat plasma by UPLC-MS/MS and its application to a pharmacokinetic study after administration of Reduning injection. Biomedical Chromatography : BMC, 29(1), 68-74. https://doi.org/10.1002/bmc.3241
Wang Y, et al. Simultaneous Determination of Neochlorogenic Acid, Chlorogenic Acid, Cryptochlorogenic Acid and Geniposide in Rat Plasma By UPLC-MS/MS and Its Application to a Pharmacokinetic Study After Administration of Reduning Injection. Biomed Chromatogr. 2015;29(1):68-74. PubMed PMID: 24842397.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous determination of neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid and geniposide in rat plasma by UPLC-MS/MS and its application to a pharmacokinetic study after administration of Reduning injection. AU - Wang,Yanjuan, AU - Wen,Jing, AU - Zheng,Weihua, AU - Zhao,Longshan, AU - Fu,Xiaohuan, AU - Wang,Zhenzhong, AU - Xiong,Zhili, AU - Li,Famei, AU - Xiao,Wei, Y1 - 2014/05/20/ PY - 2014/01/27/received PY - 2014/03/10/revised PY - 2014/04/10/accepted PY - 2014/5/21/entrez PY - 2014/5/21/pubmed PY - 2015/6/5/medline KW - Reduning injection KW - UPLC-MS/MS KW - chlorogenic acid isomers KW - geniposide KW - pharmacokinetics SP - 68 EP - 74 JF - Biomedical chromatography : BMC JO - Biomed. Chromatogr. VL - 29 IS - 1 N2 - A simple, specific and sensitive ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method was established and validated for simultaneous determination of neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid and geniposide in rat plasma using puerarin as an internal standard (IS). Plasma samples were pretreated by a one-step direct protein precipitation procedure with acetonitrile after acidified using as little as 50 μL plasma. Chromatographic separation was performed on an Acquity BEH C18 column (100 × 2.1 mm, 1.7 µm) at a flow rate of 0.2 mL/min by a gradient elution, using 0.2% acetic acid-methanol as mobile phase. The detection was performed on a triple quadrupole tandem mass spectrometer by multiple reaction monitoring via electrospray ionization source with negative ion mode. Calibration curves showed good linearity (r > 0.995) over wide concentration ranges. The intra- and inter-day precisions were <15%, and the accuracy was within ±8.0%. The validated method was successfully applied to a pharmacokinetic study of the four bioactive components in rats after intravenous administration of Reduning injection. SN - 1099-0801 UR - https://www.unboundmedicine.com/medline/citation/24842397/Simultaneous_determination_of_neochlorogenic_acid_chlorogenic_acid_cryptochlorogenic_acid_and_geniposide_in_rat_plasma_by_UPLC_MS/MS_and_its_application_to_a_pharmacokinetic_study_after_administration_of_Reduning_injection_ L2 - https://doi.org/10.1002/bmc.3241 DB - PRIME DP - Unbound Medicine ER -