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Full-thickness splinted skin wound healing models in db/db and heterozygous mice: implications for wound healing impairment.
Wound Repair Regen 2014 May-Jun; 22(3):368-80WR

Abstract

The excisional dorsal full-thickness skin wound model with or without splinting is widely utilized in wound healing studies using diabetic or normal mice. However, the effects of splinting on dermal wound healing have not been fully characterized, and there are limited data on the direct comparison of wound parameters in the splinted model between diabetic and normal mice. We compared full-thickness excisional dermal wound healing in db/db and heterozygous mice by investigating the effects of splinting, semi-occlusive dressing, and poly(ethylene glycol) treatment. Two 8-mm full-thickness wounds were made with or without splinting in db/db and heterozygous mice. Body weights, splint maintenance, wound contraction, wound closure, and histopathological parameters including reepithelialization, wound bed collagen deposition, and inflammation were compared between groups. Our results show that silicone splint application effectively reduced wound contraction in heterozygous and db/db mice. Splinted wounds, as opposed to nonsplinted wounds, exhibited no significant differences in wound closure between heterozygous and db/db mice. Finally, polyethylene glycol and the noncontact dressing had no significant effect on wound healing in heterozygous or db/db mice. We believe these findings will help investigators in selection of the appropriate wound model and data interpretation with fully defined parameters.

Authors+Show Affiliations

Department of Surgical and Radiological Sciences, School of Veterinary Medicine, University of California, Davis, California.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

24844336

Citation

Park, Shin Ae, et al. "Full-thickness Splinted Skin Wound Healing Models in Db/db and Heterozygous Mice: Implications for Wound Healing Impairment." Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society, vol. 22, no. 3, 2014, pp. 368-80.
Park SA, Teixeira LB, Raghunathan VK, et al. Full-thickness splinted skin wound healing models in db/db and heterozygous mice: implications for wound healing impairment. Wound Repair Regen. 2014;22(3):368-80.
Park, S. A., Teixeira, L. B., Raghunathan, V. K., Covert, J., Dubielzig, R. R., Isseroff, R. R., ... Murphy, C. J. (2014). Full-thickness splinted skin wound healing models in db/db and heterozygous mice: implications for wound healing impairment. Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society, 22(3), pp. 368-80. doi:10.1111/wrr.12172.
Park SA, et al. Full-thickness Splinted Skin Wound Healing Models in Db/db and Heterozygous Mice: Implications for Wound Healing Impairment. Wound Repair Regen. 2014;22(3):368-80. PubMed PMID: 24844336.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Full-thickness splinted skin wound healing models in db/db and heterozygous mice: implications for wound healing impairment. AU - Park,Shin Ae, AU - Teixeira,Leandro B C, AU - Raghunathan,Vijay Krishna, AU - Covert,Jill, AU - Dubielzig,Richard R, AU - Isseroff,Roslyn Rivkah, AU - Schurr,Michael, AU - Abbott,Nicholas L, AU - McAnulty,Jonathan, AU - Murphy,Christopher J, PY - 2013/02/05/received PY - 2014/02/27/accepted PY - 2014/5/22/entrez PY - 2014/5/23/pubmed PY - 2015/2/13/medline SP - 368 EP - 80 JF - Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society JO - Wound Repair Regen VL - 22 IS - 3 N2 - The excisional dorsal full-thickness skin wound model with or without splinting is widely utilized in wound healing studies using diabetic or normal mice. However, the effects of splinting on dermal wound healing have not been fully characterized, and there are limited data on the direct comparison of wound parameters in the splinted model between diabetic and normal mice. We compared full-thickness excisional dermal wound healing in db/db and heterozygous mice by investigating the effects of splinting, semi-occlusive dressing, and poly(ethylene glycol) treatment. Two 8-mm full-thickness wounds were made with or without splinting in db/db and heterozygous mice. Body weights, splint maintenance, wound contraction, wound closure, and histopathological parameters including reepithelialization, wound bed collagen deposition, and inflammation were compared between groups. Our results show that silicone splint application effectively reduced wound contraction in heterozygous and db/db mice. Splinted wounds, as opposed to nonsplinted wounds, exhibited no significant differences in wound closure between heterozygous and db/db mice. Finally, polyethylene glycol and the noncontact dressing had no significant effect on wound healing in heterozygous or db/db mice. We believe these findings will help investigators in selection of the appropriate wound model and data interpretation with fully defined parameters. SN - 1524-475X UR - https://www.unboundmedicine.com/medline/citation/24844336/Full_thickness_splinted_skin_wound_healing_models_in_db/db_and_heterozygous_mice:_implications_for_wound_healing_impairment_ L2 - https://doi.org/10.1111/wrr.12172 DB - PRIME DP - Unbound Medicine ER -