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Potential role of transient receptor potential (TRP) channels in bladder cancer cells.
J Physiol Sci. 2014 Jul; 64(4):305-14.JP

Abstract

Transient receptor potential (TRP) channels play important roles in thermal, chemical, and mechanical sensation in various tissues. In this study, we investigated the differences in urothelial TRP channels between normal urothelial cells and bladder cancer cells. TRPV2 and TRPM7 expression levels and TRPV2 activator-induced intracellular Ca(2+) increases were significantly higher, whereas TRPV4 expression and TRPV4 activator-induced intracellular Ca(2+) increases were significantly lower in mouse bladder cancer (MBT-2) cells compared to normal mouse urothelial cells. The proliferation rate of MBT-2 cells overexpressing dominant-negative TRPV2 was significantly increased. In contrast, treatment with TRPV2 activators significantly decreased the proliferation rate. TRPM7-overexpressing MBT-2 cells proliferated more slowly, as compared to mock-transfected cells. Moreover, expression of dominant-negative TRPV2 significantly decreased plasma membrane Ca(2+) permeability of MBT-2 cells as compared to that in mock-transfected cells. Increases in the expression of TRPV2 mRNA, immunoreactivity, and TRPV2 activator-induced intracellular Ca(2+) were also observed in T24 human bladder cancer cells. These results suggested that TRPV2 and TRPM7 were functionally expressed in bladder cancer cells and served as negative regulators of bladder cancer cell proliferation, most likely to prevent excess mechanical stresses.

Authors+Show Affiliations

Division of Cell Signaling, Okazaki Institute for Integrative Bioscience (National Institute for Physiological Sciences), National Institutes of Natural Sciences, 5-1 Higashiyama, Myodaiji, Okazaki, 444-8787, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24849279

Citation

Mizuno, Hideki, et al. "Potential Role of Transient Receptor Potential (TRP) Channels in Bladder Cancer Cells." The Journal of Physiological Sciences : JPS, vol. 64, no. 4, 2014, pp. 305-14.
Mizuno H, Suzuki Y, Watanabe M, et al. Potential role of transient receptor potential (TRP) channels in bladder cancer cells. J Physiol Sci. 2014;64(4):305-14.
Mizuno, H., Suzuki, Y., Watanabe, M., Sokabe, T., Yamamoto, T., Hattori, R., Gotoh, M., & Tominaga, M. (2014). Potential role of transient receptor potential (TRP) channels in bladder cancer cells. The Journal of Physiological Sciences : JPS, 64(4), 305-14. https://doi.org/10.1007/s12576-014-0319-6
Mizuno H, et al. Potential Role of Transient Receptor Potential (TRP) Channels in Bladder Cancer Cells. J Physiol Sci. 2014;64(4):305-14. PubMed PMID: 24849279.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Potential role of transient receptor potential (TRP) channels in bladder cancer cells. AU - Mizuno,Hideki, AU - Suzuki,Yoshiro, AU - Watanabe,Masaki, AU - Sokabe,Takaaki, AU - Yamamoto,Tokunori, AU - Hattori,Ryohei, AU - Gotoh,Momokazu, AU - Tominaga,Makoto, Y1 - 2014/05/22/ PY - 2014/01/21/received PY - 2014/05/01/accepted PY - 2014/5/23/entrez PY - 2014/5/23/pubmed PY - 2015/2/3/medline SP - 305 EP - 14 JF - The journal of physiological sciences : JPS JO - J Physiol Sci VL - 64 IS - 4 N2 - Transient receptor potential (TRP) channels play important roles in thermal, chemical, and mechanical sensation in various tissues. In this study, we investigated the differences in urothelial TRP channels between normal urothelial cells and bladder cancer cells. TRPV2 and TRPM7 expression levels and TRPV2 activator-induced intracellular Ca(2+) increases were significantly higher, whereas TRPV4 expression and TRPV4 activator-induced intracellular Ca(2+) increases were significantly lower in mouse bladder cancer (MBT-2) cells compared to normal mouse urothelial cells. The proliferation rate of MBT-2 cells overexpressing dominant-negative TRPV2 was significantly increased. In contrast, treatment with TRPV2 activators significantly decreased the proliferation rate. TRPM7-overexpressing MBT-2 cells proliferated more slowly, as compared to mock-transfected cells. Moreover, expression of dominant-negative TRPV2 significantly decreased plasma membrane Ca(2+) permeability of MBT-2 cells as compared to that in mock-transfected cells. Increases in the expression of TRPV2 mRNA, immunoreactivity, and TRPV2 activator-induced intracellular Ca(2+) were also observed in T24 human bladder cancer cells. These results suggested that TRPV2 and TRPM7 were functionally expressed in bladder cancer cells and served as negative regulators of bladder cancer cell proliferation, most likely to prevent excess mechanical stresses. SN - 1880-6562 UR - https://www.unboundmedicine.com/medline/citation/24849279/Potential_role_of_transient_receptor_potential__TRP__channels_in_bladder_cancer_cells_ L2 - https://doi.org/10.1007/s12576-014-0319-6 DB - PRIME DP - Unbound Medicine ER -