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Relationship between the ratios of CD4/CD8 T-lymphocytes in the bronchoalveolar lavage fluid and lymph nodes in patients with sarcoidosis.
Respir Investig. 2014 May; 52(3):179-83.RI

Abstract

BACKGROUND

Evaluating the ratio of CD4/CD8 T-lymphocytes in the bronchoalveolar lavage fluid (BALF) is important for understanding the clinical and pathological conditions of patients with sarcoidosis. However, few studies have thus far demonstrated the usefulness of evaluating the relationship between the ratios of CD4/CD8 T-lymphocytes in the mediastinal lymph nodes and BALF. This study aimed to investigate and identify the relationships between CD4/CD8 T-lymphocyte ratio in the mediastinal lymph nodes and BALF in patients with sarcoidosis.

METHODS

Thirty-three consecutive patients with sarcoidosis with enlarged mediastinal and/or hilar lymphadenopathy were enrolled in the study, and endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) and bronchoalveolar lavage (BAL) were simultaneously performed. The CD4/CD8 T-lymphocyte ratios in the mediastinal lymph nodes and BALF were evaluated using immunohistochemistry and flow cytometry, respectively.

RESULTS

The interobserver variability in the CD4/CD8 ratio in the mediastinal lymph nodes as determined by immunostaining was low, and the pathological and cytological profiles of T-lymphocytes in the mediastinal and/or hilar lymph nodes and BALF were correlated in patients with sarcoidosis. Additionally, the CD4/CD8 T-lymphocyte ratios in BALF were significantly higher than those in the mediastinal lymph nodes. Importantly, non-caseating granulomas were detected at a high rate by using EBUS-TBNA.

CONCLUSIONS

Performing EBUS-TBNA in patients with sarcoidosis allows correct diagnosis as well as the estimation of the ratio of CD4/CD8 T-lymphocytes in BALF.

Authors+Show Affiliations

Department of Respiratory Medicine, University of Occupational and Environmental Health, 1-1, Iseigaoka, Yahatanishiku, Kitakyushu City, Fukuoka 807-8555, Japan. Electronic address: oda-keishi@med.uoeh-u.ac.jp.Department of Respiratory Medicine, University of Occupational and Environmental Health, 1-1, Iseigaoka, Yahatanishiku, Kitakyushu City, Fukuoka 807-8555, Japan. Electronic address: h-ishimoto@med.uoeh-u.ac.jp.Department of Respiratory Medicine, University of Occupational and Environmental Health, 1-1, Iseigaoka, Yahatanishiku, Kitakyushu City, Fukuoka 807-8555, Japan. Electronic address: yatera@med.uoeh-u.ac.jp.Department of Pathology and Cell Biology, University of Occupational and Environmental Health, 1-1, Iseigaoka, Yahatanishiku, Kitakyushu City, Fukuoka 807-8555, Japan. Electronic address: sousuke@med.uoeh-u.ac.jp.Department of Epidemiology, National Institute of Public Health, 2-3-6 Minami, Wako-shi, Saitama 351-0197, Japan. Electronic address: nakaohi@niph.go.jp.Department of Respiratory Medicine, University of Occupational and Environmental Health, 1-1, Iseigaoka, Yahatanishiku, Kitakyushu City, Fukuoka 807-8555, Japan. Electronic address: togoshi@ybb.ne.jp.Department of Respiratory Medicine, University of Occupational and Environmental Health, 1-1, Iseigaoka, Yahatanishiku, Kitakyushu City, Fukuoka 807-8555, Japan. Electronic address: sn0920@med.uoeh-u.ac.jp.Department of Respiratory Medicine, University of Occupational and Environmental Health, 1-1, Iseigaoka, Yahatanishiku, Kitakyushu City, Fukuoka 807-8555, Japan. Electronic address: kkyamsaki1019@yahoo.co.jp.Department of Respiratory Medicine, University of Occupational and Environmental Health, 1-1, Iseigaoka, Yahatanishiku, Kitakyushu City, Fukuoka 807-8555, Japan. Electronic address: namihei@med.uoeh-u.ac.jp.Department of Respiratory Medicine, University of Occupational and Environmental Health, 1-1, Iseigaoka, Yahatanishiku, Kitakyushu City, Fukuoka 807-8555, Japan. Electronic address: hmukae@med.uoeh-u.ac.jp.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

24853018

Citation

Oda, Keishi, et al. "Relationship Between the Ratios of CD4/CD8 T-lymphocytes in the Bronchoalveolar Lavage Fluid and Lymph Nodes in Patients With Sarcoidosis." Respiratory Investigation, vol. 52, no. 3, 2014, pp. 179-83.
Oda K, Ishimoto H, Yatera K, et al. Relationship between the ratios of CD4/CD8 T-lymphocytes in the bronchoalveolar lavage fluid and lymph nodes in patients with sarcoidosis. Respir Investig. 2014;52(3):179-83.
Oda, K., Ishimoto, H., Yatera, K., Yamada, S., Nakao, H., Ogoshi, T., Noguchi, S., Yamasaki, K., Kawanami, T., & Mukae, H. (2014). Relationship between the ratios of CD4/CD8 T-lymphocytes in the bronchoalveolar lavage fluid and lymph nodes in patients with sarcoidosis. Respiratory Investigation, 52(3), 179-83. https://doi.org/10.1016/j.resinv.2013.12.003
Oda K, et al. Relationship Between the Ratios of CD4/CD8 T-lymphocytes in the Bronchoalveolar Lavage Fluid and Lymph Nodes in Patients With Sarcoidosis. Respir Investig. 2014;52(3):179-83. PubMed PMID: 24853018.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Relationship between the ratios of CD4/CD8 T-lymphocytes in the bronchoalveolar lavage fluid and lymph nodes in patients with sarcoidosis. AU - Oda,Keishi, AU - Ishimoto,Hiroshi, AU - Yatera,Kazuhiro, AU - Yamada,Sohsuke, AU - Nakao,Hiroyuki, AU - Ogoshi,Takaaki, AU - Noguchi,Shingo, AU - Yamasaki,Kei, AU - Kawanami,Toshinori, AU - Mukae,Hiroshi, Y1 - 2014/01/13/ PY - 2013/06/10/received PY - 2013/12/03/revised PY - 2013/12/04/accepted PY - 2014/5/24/entrez PY - 2014/5/24/pubmed PY - 2015/9/1/medline KW - Bronchoalveolar lavage fluid KW - CD4/CD8 ratio KW - Endobronchial ultrasound-guided transbronchial needle aspiration KW - Sarcoidosis SP - 179 EP - 83 JF - Respiratory investigation JO - Respir Investig VL - 52 IS - 3 N2 - BACKGROUND: Evaluating the ratio of CD4/CD8 T-lymphocytes in the bronchoalveolar lavage fluid (BALF) is important for understanding the clinical and pathological conditions of patients with sarcoidosis. However, few studies have thus far demonstrated the usefulness of evaluating the relationship between the ratios of CD4/CD8 T-lymphocytes in the mediastinal lymph nodes and BALF. This study aimed to investigate and identify the relationships between CD4/CD8 T-lymphocyte ratio in the mediastinal lymph nodes and BALF in patients with sarcoidosis. METHODS: Thirty-three consecutive patients with sarcoidosis with enlarged mediastinal and/or hilar lymphadenopathy were enrolled in the study, and endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) and bronchoalveolar lavage (BAL) were simultaneously performed. The CD4/CD8 T-lymphocyte ratios in the mediastinal lymph nodes and BALF were evaluated using immunohistochemistry and flow cytometry, respectively. RESULTS: The interobserver variability in the CD4/CD8 ratio in the mediastinal lymph nodes as determined by immunostaining was low, and the pathological and cytological profiles of T-lymphocytes in the mediastinal and/or hilar lymph nodes and BALF were correlated in patients with sarcoidosis. Additionally, the CD4/CD8 T-lymphocyte ratios in BALF were significantly higher than those in the mediastinal lymph nodes. Importantly, non-caseating granulomas were detected at a high rate by using EBUS-TBNA. CONCLUSIONS: Performing EBUS-TBNA in patients with sarcoidosis allows correct diagnosis as well as the estimation of the ratio of CD4/CD8 T-lymphocytes in BALF. SN - 2212-5353 UR - https://www.unboundmedicine.com/medline/citation/24853018/Relationship_between_the_ratios_of_CD4/CD8_T_lymphocytes_in_the_bronchoalveolar_lavage_fluid_and_lymph_nodes_in_patients_with_sarcoidosis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S2212-5345(13)00160-3 DB - PRIME DP - Unbound Medicine ER -