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Prussian blue-gold nanoparticles-ionic liquid functionalized reduced graphene oxide nanocomposite as label for ultrasensitive electrochemical immunoassay of alpha-fetoprotein.
Anal Chim Acta. 2014 Jun 04; 829:15-21.AC

Abstract

In this work, poly(diallyldimethylammonium chloride) (PDDA) protected Prussian blue/gold nanoparticles/ionic liquid functionalized reduced graphene oxide (IL-rGO-Au-PDDA-PB) nanocomposite was fabricated. The resulting nanocomposite exhibited high biocompatibility, conductivity and catalytic activity. To assess the performance of the nanocomposite, a sensitive sandwich-type immunosensor was constructed for detecting alpha-fetoprotein (AFP). Greatly enhanced sensitivity for this immunosensor was based on triple signal amplification strategies. Firstly, IL-rGO modified electrode was used as biosensor platform to capture a large amount of antibody due to its increased surface area, thus amplifying the detection response. Secondly, a large number of Au-PDDA-PB was conjugated on the surface of IL-rGO, which meant the enrichment of the signal and the more immobilization of label antibody. Finally, the catalytic reaction between H2O2 and the IL-rGO-Au-PDDA-PB nanocomposite further enhanced the signal response. The signals increased linearly with AFP concentrations in the range of 0.01-100 ng mL(-1). The detection limit for AFP was 4.6 pg mL(-1). The immunosensor showed high sensitivity, excellent selectivity and good stability. Moreover, the immunosensor was applied to the analysis of AFP in serum sample with satisfactory result.

Authors+Show Affiliations

Department of Chemistry, Capital Normal University, Beijing, 100048, China.Department of Chemistry, Capital Normal University, Beijing, 100048, China.Department of Chemistry, Capital Normal University, Beijing, 100048, China. Electronic address: mazhanfang@cnu.edu.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24856397

Citation

Gao, Qi, et al. "Prussian Blue-gold Nanoparticles-ionic Liquid Functionalized Reduced Graphene Oxide Nanocomposite as Label for Ultrasensitive Electrochemical Immunoassay of Alpha-fetoprotein." Analytica Chimica Acta, vol. 829, 2014, pp. 15-21.
Gao Q, Liu N, Ma Z. Prussian blue-gold nanoparticles-ionic liquid functionalized reduced graphene oxide nanocomposite as label for ultrasensitive electrochemical immunoassay of alpha-fetoprotein. Anal Chim Acta. 2014;829:15-21.
Gao, Q., Liu, N., & Ma, Z. (2014). Prussian blue-gold nanoparticles-ionic liquid functionalized reduced graphene oxide nanocomposite as label for ultrasensitive electrochemical immunoassay of alpha-fetoprotein. Analytica Chimica Acta, 829, 15-21. https://doi.org/10.1016/j.aca.2014.04.045
Gao Q, Liu N, Ma Z. Prussian Blue-gold Nanoparticles-ionic Liquid Functionalized Reduced Graphene Oxide Nanocomposite as Label for Ultrasensitive Electrochemical Immunoassay of Alpha-fetoprotein. Anal Chim Acta. 2014 Jun 4;829:15-21. PubMed PMID: 24856397.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Prussian blue-gold nanoparticles-ionic liquid functionalized reduced graphene oxide nanocomposite as label for ultrasensitive electrochemical immunoassay of alpha-fetoprotein. AU - Gao,Qi, AU - Liu,Na, AU - Ma,Zhanfang, Y1 - 2014/04/26/ PY - 2013/12/26/received PY - 2014/04/17/revised PY - 2014/04/21/accepted PY - 2014/5/27/entrez PY - 2014/5/27/pubmed PY - 2015/5/12/medline KW - Alpha-fetoprotein KW - Electrochemical immunosensor KW - Ionic liquid functionalized reduced graphene oxide KW - Prussian blue SP - 15 EP - 21 JF - Analytica chimica acta JO - Anal. Chim. Acta VL - 829 N2 - In this work, poly(diallyldimethylammonium chloride) (PDDA) protected Prussian blue/gold nanoparticles/ionic liquid functionalized reduced graphene oxide (IL-rGO-Au-PDDA-PB) nanocomposite was fabricated. The resulting nanocomposite exhibited high biocompatibility, conductivity and catalytic activity. To assess the performance of the nanocomposite, a sensitive sandwich-type immunosensor was constructed for detecting alpha-fetoprotein (AFP). Greatly enhanced sensitivity for this immunosensor was based on triple signal amplification strategies. Firstly, IL-rGO modified electrode was used as biosensor platform to capture a large amount of antibody due to its increased surface area, thus amplifying the detection response. Secondly, a large number of Au-PDDA-PB was conjugated on the surface of IL-rGO, which meant the enrichment of the signal and the more immobilization of label antibody. Finally, the catalytic reaction between H2O2 and the IL-rGO-Au-PDDA-PB nanocomposite further enhanced the signal response. The signals increased linearly with AFP concentrations in the range of 0.01-100 ng mL(-1). The detection limit for AFP was 4.6 pg mL(-1). The immunosensor showed high sensitivity, excellent selectivity and good stability. Moreover, the immunosensor was applied to the analysis of AFP in serum sample with satisfactory result. SN - 1873-4324 UR - https://www.unboundmedicine.com/medline/citation/24856397/Prussian_blue_gold_nanoparticles_ionic_liquid_functionalized_reduced_graphene_oxide_nanocomposite_as_label_for_ultrasensitive_electrochemical_immunoassay_of_alpha_fetoprotein_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-2670(14)00499-1 DB - PRIME DP - Unbound Medicine ER -