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Correlation between lipid profile and heme oxygenase system in patients with benign prostatic hyperplasia.
Urology. 2014 Jun; 83(6):1444.e7-13.U

Abstract

OBJECTIVE

To investigate the role of heme oxygenase (HO) system in moderate to severe benign prostatic hyperplasia and lower urinary tract symptom patients and the influence of metabolic syndrome (MetS) components on HO-1 or HO-2 prostatic levels.

METHODS

One hundred thirty-two consecutive patients who underwent transurethral resection of the prostate were prospectively enrolled. MetS was defined by the International Diabetes Federation. Patients were divided in 2 groups: group A (high-density lipoprotein-cholesterol [HDL-C]≥40 mg/dL and triglycerides<150 mg/dL) and group B (HDL-C<40 mg/dL and triglycerides≥150 mg/dL). Surgical specimens were collected for HO level determination. HO-1 levels were determined by enzyme-linked immunosorbent assay and HO-1 levels by Western blotting.

RESULTS

Patients with MetS showed lower levels of HO-1 (5.29 vs 6.28 ng/mL; P=.04), HO-2 (1.01 vs 1.83 ng/mL; P=.04), phosphorylated activated protein kinase (pAMPK; 0.62 vs 1.11 AUI; P<.01), and HO-activity (61.43 vs 70.22 AUI; P<.01) with respect to normal. The Pearson correlation analysis showed that HO-1, HO-2, and HO activity were negatively associated with waist circumference (P<.05), body mass index (P<.05), triglycerides (P<.05) and positively with HDL-C (P<.05). Group B showed lower levels of HO-1 (4.7 vs 6.6 ng/mL; P<.05), HO-2 (1.4 vs 0.4 ng/mL; P=.03), HO-activity (69.63 vs 58.42 AUI; P=.04), and higher International Prostate Symptoms Score (21.4 vs 25.0; P<.05) with respect to group A. The enzyme-linked immunosorbent assay showed that HO-1 and HO activity levels were significantly lower in group B compared with group A. Reduced HDL-C and elevated triglyceride levels decreased HO-1 expression in the prostate tissue. Western blot analysis of tissue samples showed significant differences in basal protein expression levels of HO-2 and pAMPK in group B compared with group A.

CONCLUSION

Alteration of serum triglycerides and HDL-C significantly impairs HO-1 and HO-2 levels in benign prostatic hyperplasia patients.

Authors+Show Affiliations

Department of Drug Science, Biochemistry Section, University of Catania, Catania, Italy.Department of Urology, Policlinico Hospital, University of Catania, Catania, Italy. Electronic address: giorgioivan@virgilio.it.Department of Urology, Policlinico Hospital, University of Catania, Catania, Italy.Department of Urology, Policlinico Hospital, University of Catania, Catania, Italy.Department of Urology, Policlinico Hospital, University of Catania, Catania, Italy.Department of Drug Science, Biochemistry Section, University of Catania, Catania, Italy.Department of Drug Science, Biochemistry Section, University of Catania, Catania, Italy.Department of Drug Science, Biochemistry Section, University of Catania, Catania, Italy.Department of Urology, Policlinico Hospital, University of Catania, Catania, Italy.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

24862399

Citation

Vanella, Luca, et al. "Correlation Between Lipid Profile and Heme Oxygenase System in Patients With Benign Prostatic Hyperplasia." Urology, vol. 83, no. 6, 2014, pp. 1444.e7-13.
Vanella L, Russo GI, Cimino S, et al. Correlation between lipid profile and heme oxygenase system in patients with benign prostatic hyperplasia. Urology. 2014;83(6):1444.e7-13.
Vanella, L., Russo, G. I., Cimino, S., Fragalà, E., Favilla, V., Li Volti, G., Barbagallo, I., Sorrenti, V., & Morgia, G. (2014). Correlation between lipid profile and heme oxygenase system in patients with benign prostatic hyperplasia. Urology, 83(6), e7-13. https://doi.org/10.1016/j.urology.2014.03.007
Vanella L, et al. Correlation Between Lipid Profile and Heme Oxygenase System in Patients With Benign Prostatic Hyperplasia. Urology. 2014;83(6):1444.e7-13. PubMed PMID: 24862399.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Correlation between lipid profile and heme oxygenase system in patients with benign prostatic hyperplasia. AU - Vanella,Luca, AU - Russo,Giorgio Ivan, AU - Cimino,Sebastiano, AU - Fragalà,Eugenia, AU - Favilla,Vincenzo, AU - Li Volti,Giovanni, AU - Barbagallo,Ignazio, AU - Sorrenti,Valeria, AU - Morgia,Giuseppe, PY - 2014/01/21/received PY - 2014/03/07/revised PY - 2014/03/10/accepted PY - 2014/5/28/entrez PY - 2014/5/28/pubmed PY - 2014/8/6/medline SP - 1444.e7 EP - 13 JF - Urology JO - Urology VL - 83 IS - 6 N2 - OBJECTIVE: To investigate the role of heme oxygenase (HO) system in moderate to severe benign prostatic hyperplasia and lower urinary tract symptom patients and the influence of metabolic syndrome (MetS) components on HO-1 or HO-2 prostatic levels. METHODS: One hundred thirty-two consecutive patients who underwent transurethral resection of the prostate were prospectively enrolled. MetS was defined by the International Diabetes Federation. Patients were divided in 2 groups: group A (high-density lipoprotein-cholesterol [HDL-C]≥40 mg/dL and triglycerides<150 mg/dL) and group B (HDL-C<40 mg/dL and triglycerides≥150 mg/dL). Surgical specimens were collected for HO level determination. HO-1 levels were determined by enzyme-linked immunosorbent assay and HO-1 levels by Western blotting. RESULTS: Patients with MetS showed lower levels of HO-1 (5.29 vs 6.28 ng/mL; P=.04), HO-2 (1.01 vs 1.83 ng/mL; P=.04), phosphorylated activated protein kinase (pAMPK; 0.62 vs 1.11 AUI; P<.01), and HO-activity (61.43 vs 70.22 AUI; P<.01) with respect to normal. The Pearson correlation analysis showed that HO-1, HO-2, and HO activity were negatively associated with waist circumference (P<.05), body mass index (P<.05), triglycerides (P<.05) and positively with HDL-C (P<.05). Group B showed lower levels of HO-1 (4.7 vs 6.6 ng/mL; P<.05), HO-2 (1.4 vs 0.4 ng/mL; P=.03), HO-activity (69.63 vs 58.42 AUI; P=.04), and higher International Prostate Symptoms Score (21.4 vs 25.0; P<.05) with respect to group A. The enzyme-linked immunosorbent assay showed that HO-1 and HO activity levels were significantly lower in group B compared with group A. Reduced HDL-C and elevated triglyceride levels decreased HO-1 expression in the prostate tissue. Western blot analysis of tissue samples showed significant differences in basal protein expression levels of HO-2 and pAMPK in group B compared with group A. CONCLUSION: Alteration of serum triglycerides and HDL-C significantly impairs HO-1 and HO-2 levels in benign prostatic hyperplasia patients. SN - 1527-9995 UR - https://www.unboundmedicine.com/medline/citation/24862399/Correlation_between_lipid_profile_and_heme_oxygenase_system_in_patients_with_benign_prostatic_hyperplasia_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0090-4295(14)00250-7 DB - PRIME DP - Unbound Medicine ER -