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Val66Met polymorphism in the BDNF gene in children with bronchial asthma.
Pediatr Pulmonol 2015; 50(7):631-7PP

Abstract

OBJECTIVES

Bronchial asthma is a chronic respiratory disease characterized by airway inflammation. There is increasing evidence that neurotrophins play an important role in the development and maintenance of neurogenic airway inflammation in chronic allergic diseases.

WORKING HYPOTHESIS

Brain-derived neurotrophic factor (BDNF) is a member of the neurotrophin family and has several important functions in the airways. There are only a few reports on the association between genetic variations in the BDNF gene and various allergic diseases, and the results are generally conflicting. Therefore, we aimed to study the functional polymorphism Val66Met (also called rs6265 or G196A) in the BDNF gene in a group of asthmatic children and healthy controls.

STUDY DESIGN, PATIENT-SELECTION, AND METHODOLOGY

We studied 248 asthmatic patients (aged 12.28 ± 0.24 years) and 249 healthy children (aged 13.14 ± 0.48 years). Analysis of the Val66Met polymorphism of the BDNF gene was performed by polymerase chain reaction (PCR) and PCR products were digested by PmlI.

RESULTS

The prevalence of the Val66Met polymorphisms (Val/Val, Val/Met, and Met/Met) was 61.7%, 33.5%, and 4.8% in asthmatics, respectively, and 47.0%, 51.8%, and 1.2% in healthy subjects, respectively. We observed a significant association of the Met/Met variant genotype with asthmatics (OR = 4.17, 95% CI = 1.16-14.96, P = 0.018). The Val/Met genotype was protective against bronchial asthma (OR = 0.69, 95% CI = 0.48-0.99, P = 0.045), especially in girls (OR = 0.34, 95% CI = 0.20-0.59, P = 0.001).

CONCLUSION

Specific BDNF gene polymorphism may contribute to bronchial asthma susceptibility. Our study suggested the positive association between selected functional BDNF polymorphism (rs6265) and asthma in children.

Authors+Show Affiliations

Department of Paediatrics, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Martin, Slovakia.Department of Medical Biochemistry, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Martin, Slovakia.Department of Medical Biochemistry, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Martin, Slovakia.Department of Paediatrics, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Martin, Slovakia.Department of Medical Biochemistry, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Martin, Slovakia.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24863266

Citation

Jesenak, Milos, et al. "Val66Met Polymorphism in the BDNF Gene in Children With Bronchial Asthma." Pediatric Pulmonology, vol. 50, no. 7, 2015, pp. 631-7.
Jesenak M, Babusikova E, Evinova A, et al. Val66Met polymorphism in the BDNF gene in children with bronchial asthma. Pediatr Pulmonol. 2015;50(7):631-7.
Jesenak, M., Babusikova, E., Evinova, A., Banovcin, P., & Dobrota, D. (2015). Val66Met polymorphism in the BDNF gene in children with bronchial asthma. Pediatric Pulmonology, 50(7), pp. 631-7. doi:10.1002/ppul.23065.
Jesenak M, et al. Val66Met Polymorphism in the BDNF Gene in Children With Bronchial Asthma. Pediatr Pulmonol. 2015;50(7):631-7. PubMed PMID: 24863266.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Val66Met polymorphism in the BDNF gene in children with bronchial asthma. AU - Jesenak,Milos, AU - Babusikova,Eva, AU - Evinova,Andrea, AU - Banovcin,Peter, AU - Dobrota,Dusan, Y1 - 2014/05/26/ PY - 2013/05/06/received PY - 2014/04/21/accepted PY - 2014/5/28/entrez PY - 2014/5/28/pubmed PY - 2016/4/8/medline KW - brain-derived neurotrophic factor KW - bronchial asthma KW - children KW - gene polymorphism KW - neurogenic inflammation KW - neurotrophins SP - 631 EP - 7 JF - Pediatric pulmonology JO - Pediatr. Pulmonol. VL - 50 IS - 7 N2 - OBJECTIVES: Bronchial asthma is a chronic respiratory disease characterized by airway inflammation. There is increasing evidence that neurotrophins play an important role in the development and maintenance of neurogenic airway inflammation in chronic allergic diseases. WORKING HYPOTHESIS: Brain-derived neurotrophic factor (BDNF) is a member of the neurotrophin family and has several important functions in the airways. There are only a few reports on the association between genetic variations in the BDNF gene and various allergic diseases, and the results are generally conflicting. Therefore, we aimed to study the functional polymorphism Val66Met (also called rs6265 or G196A) in the BDNF gene in a group of asthmatic children and healthy controls. STUDY DESIGN, PATIENT-SELECTION, AND METHODOLOGY: We studied 248 asthmatic patients (aged 12.28 ± 0.24 years) and 249 healthy children (aged 13.14 ± 0.48 years). Analysis of the Val66Met polymorphism of the BDNF gene was performed by polymerase chain reaction (PCR) and PCR products were digested by PmlI. RESULTS: The prevalence of the Val66Met polymorphisms (Val/Val, Val/Met, and Met/Met) was 61.7%, 33.5%, and 4.8% in asthmatics, respectively, and 47.0%, 51.8%, and 1.2% in healthy subjects, respectively. We observed a significant association of the Met/Met variant genotype with asthmatics (OR = 4.17, 95% CI = 1.16-14.96, P = 0.018). The Val/Met genotype was protective against bronchial asthma (OR = 0.69, 95% CI = 0.48-0.99, P = 0.045), especially in girls (OR = 0.34, 95% CI = 0.20-0.59, P = 0.001). CONCLUSION: Specific BDNF gene polymorphism may contribute to bronchial asthma susceptibility. Our study suggested the positive association between selected functional BDNF polymorphism (rs6265) and asthma in children. SN - 1099-0496 UR - https://www.unboundmedicine.com/medline/citation/24863266/Val66Met_polymorphism_in_the_BDNF_gene_in_children_with_bronchial_asthma_ L2 - https://doi.org/10.1002/ppul.23065 DB - PRIME DP - Unbound Medicine ER -