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CO2 biofixation and carbonic anhydrase activity in Scenedesmus obliquus SA1 cultivated in large scale open system.
Bioresour Technol. 2014 Jul; 164:323-30.BT

Abstract

The present study deals with the large scale open system cultivation of the novel microalga: Scenedesmus obliquus SA1 (KC733762) previously isolated in our laboratory. SA1 strain was cultivated in open system at varying CO2 levels ranging from 0.03% to 35% (v/v) and subsequently the carbonic anhydrase activity (CA) and the biochemical properties were monitored. Maximum biomass concentration (1.39 ± 0.023 g L(-1)), CO2 fixation rate (97.65 ± 1.03 mg L(-1)d(-1)) and total CA activity (166.86 ± 3.30 E.U./mg chla) were obtained at 35% CO2. CA inhibitors: acetazolamide and ethoxyzolamide inhibited the external and internal enzyme activity in SA1. High CO2 levels were favorable for the accumulation of lipids and chlorophyll. The present results suggested that SA1 possessed high CO2 tolerance and high carbohydrate, lipid and chlorophyll content when cultivated in open system thus being suitable for CO2 mitigation in outdoor ponds and subsequent generation of value added products.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Basu S
Centre for Environment, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India.
Roy AS
Centre for Energy, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India.
Mohanty K
Department of Chemical Engineering, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India.
Ghoshal AK
Centre for Energy, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India; Department of Chemical Engineering, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India. Electronic address: aloke@iitg.ernet.in.

MeSH

BiomassCarbon CycleCarbon DioxideCarbonic Anhydrase InhibitorsCarbonic AnhydrasesCell Culture TechniquesKineticsScenedesmusTime Factors

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24865325

Citation

Basu, Samarpita, et al. "CO2 Biofixation and Carbonic Anhydrase Activity in Scenedesmus Obliquus SA1 Cultivated in Large Scale Open System." Bioresource Technology, vol. 164, 2014, pp. 323-30.
Basu S, Roy AS, Mohanty K, et al. CO2 biofixation and carbonic anhydrase activity in Scenedesmus obliquus SA1 cultivated in large scale open system. Bioresour Technol. 2014;164:323-30.
Basu, S., Roy, A. S., Mohanty, K., & Ghoshal, A. K. (2014). CO2 biofixation and carbonic anhydrase activity in Scenedesmus obliquus SA1 cultivated in large scale open system. Bioresource Technology, 164, 323-30. https://doi.org/10.1016/j.biortech.2014.05.017
Basu S, et al. CO2 Biofixation and Carbonic Anhydrase Activity in Scenedesmus Obliquus SA1 Cultivated in Large Scale Open System. Bioresour Technol. 2014;164:323-30. PubMed PMID: 24865325.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - CO2 biofixation and carbonic anhydrase activity in Scenedesmus obliquus SA1 cultivated in large scale open system. AU - Basu,Samarpita, AU - Roy,Abhijit Sarma, AU - Mohanty,Kaustubha, AU - Ghoshal,Aloke K, Y1 - 2014/05/13/ PY - 2014/03/20/received PY - 2014/05/03/revised PY - 2014/05/05/accepted PY - 2014/5/29/entrez PY - 2014/5/29/pubmed PY - 2015/1/28/medline KW - CO(2) biofixation KW - Carbonic anhydrase KW - Microalgae KW - Open system SP - 323 EP - 30 JF - Bioresource technology JO - Bioresour Technol VL - 164 N2 - The present study deals with the large scale open system cultivation of the novel microalga: Scenedesmus obliquus SA1 (KC733762) previously isolated in our laboratory. SA1 strain was cultivated in open system at varying CO2 levels ranging from 0.03% to 35% (v/v) and subsequently the carbonic anhydrase activity (CA) and the biochemical properties were monitored. Maximum biomass concentration (1.39 ± 0.023 g L(-1)), CO2 fixation rate (97.65 ± 1.03 mg L(-1)d(-1)) and total CA activity (166.86 ± 3.30 E.U./mg chla) were obtained at 35% CO2. CA inhibitors: acetazolamide and ethoxyzolamide inhibited the external and internal enzyme activity in SA1. High CO2 levels were favorable for the accumulation of lipids and chlorophyll. The present results suggested that SA1 possessed high CO2 tolerance and high carbohydrate, lipid and chlorophyll content when cultivated in open system thus being suitable for CO2 mitigation in outdoor ponds and subsequent generation of value added products. SN - 1873-2976 UR - https://www.unboundmedicine.com/medline/citation/24865325/CO2_biofixation_and_carbonic_anhydrase_activity_in_Scenedesmus_obliquus_SA1_cultivated_in_large_scale_open_system_ DB - PRIME DP - Unbound Medicine ER -