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Survey for hantaviruses, tick-borne encephalitis virus, and Rickettsia spp. in small rodents in Croatia.
Vector Borne Zoonotic Dis. 2014 Jul; 14(7):523-30.VB

Abstract

In Croatia, several rodent- and vector-borne agents are endemic and of medical importance. In this study, we investigated hantaviruses and, for the first time, tick-borne encephalitis virus (TBEV) and Rickettsia spp. in small wild rodents from two different sites (mountainous and lowland region) in Croatia. In total, 194 transudate and tissue samples from 170 rodents (A. flavicollis, n=115; A. agrarius, n=2; Myodes glareolus, n=53) were tested for antibodies by indirect immunofluorescence assays (IIFT) and for nucleic acids by conventional (hantaviruses) and real-time RT-/PCRs (TBEV and Rickettsia spp.). A total of 25.5% (24/94) of the rodents from the mountainous area revealed specific antibodies against hantaviruses. In all, 21.3% (20/94) of the samples from the mountainous area and 29.0% (9/31) from the lowland area yielded positive results for either Puumala virus (PUUV) or Dobrava-Belgrade virus (DOBV) using a conventional RT-PCR. All processed samples (n=194) were negative for TBEV by IIFT or real-time RT-PCR. Serological evidence of rickettsial infection was detected in 4.3% (4/94) rodents from the mountainous region. Another 3.2% (3/94) rodents were positive for Rickettsia spp. by real-time PCR. None of the rodents (n=76) from the lowland area were positive for Rickettsia spp. by real-time PCR. Dual infection of PUUV and Rickettsia spp. was found in one M. glareolus from the mountainous area by RT-PCR and real-time PCR, respectively. To our knowledge, this is the first detection of Rickettsia spp. in small rodents from Croatia. Phylogenetic analyses of S- and M-segment sequences obtained from the two study sites revealed well-supported subgroups in Croatian PUUV and DOBV. Although somewhat limited, our data showed occurrence and prevalence of PUUV, DOBV, and rickettsiae in Croatia. Further studies are warranted to confirm these data and to determine the Rickettsia species present in rodents in these areas.

Authors+Show Affiliations

1 University Hospital for Infectious Diseases "Dr. Fran Mihaljević" , Research Department, Zagreb, Croatia .No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24866325

Citation

Svoboda, Petra, et al. "Survey for Hantaviruses, Tick-borne Encephalitis Virus, and Rickettsia Spp. in Small Rodents in Croatia." Vector Borne and Zoonotic Diseases (Larchmont, N.Y.), vol. 14, no. 7, 2014, pp. 523-30.
Svoboda P, Dobler G, Markotić A, et al. Survey for hantaviruses, tick-borne encephalitis virus, and Rickettsia spp. in small rodents in Croatia. Vector Borne Zoonotic Dis. 2014;14(7):523-30.
Svoboda, P., Dobler, G., Markotić, A., Kurolt, I. C., Speck, S., Habuš, J., Vucelja, M., Krajinović, L. C., Tadin, A., Margaletić, J., & Essbauer, S. (2014). Survey for hantaviruses, tick-borne encephalitis virus, and Rickettsia spp. in small rodents in Croatia. Vector Borne and Zoonotic Diseases (Larchmont, N.Y.), 14(7), 523-30. https://doi.org/10.1089/vbz.2013.1457
Svoboda P, et al. Survey for Hantaviruses, Tick-borne Encephalitis Virus, and Rickettsia Spp. in Small Rodents in Croatia. Vector Borne Zoonotic Dis. 2014;14(7):523-30. PubMed PMID: 24866325.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Survey for hantaviruses, tick-borne encephalitis virus, and Rickettsia spp. in small rodents in Croatia. AU - Svoboda,Petra, AU - Dobler,Gerhard, AU - Markotić,Alemka, AU - Kurolt,Ivan-Christian, AU - Speck,Stephanie, AU - Habuš,Josipa, AU - Vucelja,Marko, AU - Krajinović,Lidija Cvetko, AU - Tadin,Ante, AU - Margaletić,Josip, AU - Essbauer,Sandra, Y1 - 2014/05/27/ PY - 2014/5/29/entrez PY - 2014/5/29/pubmed PY - 2015/4/17/medline KW - Hantavirus KW - Rickettsia KW - Rodents KW - Tick-borne encephalitis virus KW - Zoonosis SP - 523 EP - 30 JF - Vector borne and zoonotic diseases (Larchmont, N.Y.) JO - Vector Borne Zoonotic Dis. VL - 14 IS - 7 N2 - In Croatia, several rodent- and vector-borne agents are endemic and of medical importance. In this study, we investigated hantaviruses and, for the first time, tick-borne encephalitis virus (TBEV) and Rickettsia spp. in small wild rodents from two different sites (mountainous and lowland region) in Croatia. In total, 194 transudate and tissue samples from 170 rodents (A. flavicollis, n=115; A. agrarius, n=2; Myodes glareolus, n=53) were tested for antibodies by indirect immunofluorescence assays (IIFT) and for nucleic acids by conventional (hantaviruses) and real-time RT-/PCRs (TBEV and Rickettsia spp.). A total of 25.5% (24/94) of the rodents from the mountainous area revealed specific antibodies against hantaviruses. In all, 21.3% (20/94) of the samples from the mountainous area and 29.0% (9/31) from the lowland area yielded positive results for either Puumala virus (PUUV) or Dobrava-Belgrade virus (DOBV) using a conventional RT-PCR. All processed samples (n=194) were negative for TBEV by IIFT or real-time RT-PCR. Serological evidence of rickettsial infection was detected in 4.3% (4/94) rodents from the mountainous region. Another 3.2% (3/94) rodents were positive for Rickettsia spp. by real-time PCR. None of the rodents (n=76) from the lowland area were positive for Rickettsia spp. by real-time PCR. Dual infection of PUUV and Rickettsia spp. was found in one M. glareolus from the mountainous area by RT-PCR and real-time PCR, respectively. To our knowledge, this is the first detection of Rickettsia spp. in small rodents from Croatia. Phylogenetic analyses of S- and M-segment sequences obtained from the two study sites revealed well-supported subgroups in Croatian PUUV and DOBV. Although somewhat limited, our data showed occurrence and prevalence of PUUV, DOBV, and rickettsiae in Croatia. Further studies are warranted to confirm these data and to determine the Rickettsia species present in rodents in these areas. SN - 1557-7759 UR - https://www.unboundmedicine.com/medline/citation/24866325/Survey_for_hantaviruses_tick_borne_encephalitis_virus_and_Rickettsia_spp__in_small_rodents_in_Croatia_ L2 - https://www.liebertpub.com/doi/full/10.1089/vbz.2013.1457?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -