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Burkholderia pseudomallei capsular polysaccharide conjugates provide protection against acute melioidosis.
Infect Immun. 2014 Aug; 82(8):3206-13.II

Abstract

Burkholderia pseudomallei, the etiologic agent of melioidosis, is a CDC tier 1 select agent that causes severe disease in both humans and animals. Diagnosis and treatment of melioidosis can be challenging, and in the absence of optimal chemotherapeutic intervention, acute disease is frequently fatal. Melioidosis is an emerging infectious disease for which there are currently no licensed vaccines. Due to the potential malicious use of B. pseudomallei as well as its impact on public health in regions where the disease is endemic, there is significant interest in developing vaccines for immunization against this disease. In the present study, type A O-polysaccharide (OPS) and manno-heptose capsular polysaccharide (CPS) antigens were isolated from nonpathogenic, select-agent-excluded strains of B. pseudomallei and covalently linked to carrier proteins. By using these conjugates (OPS2B1 and CPS2B1, respectively), it was shown that although high-titer IgG responses against the OPS or CPS component of the glycoconjugates could be raised in BALB/c mice, only those animals immunized with CPS2B1 were protected against intraperitoneal challenge with B. pseudomallei. Extending upon these studies, it was also demonstrated that when the mice were immunized with a combination of CPS2B1 and recombinant B. pseudomallei LolC, rather than with CPS2B1 or LolC individually, they exhibited higher survival rates when challenged with a lethal dose of B. pseudomallei. Collectively, these results suggest that CPS-based glycoconjugates are promising candidates for the development of subunit vaccines for immunization against melioidosis.

Authors+Show Affiliations

Defence Science and Technology Laboratory, Porton Down, Salisbury, United Kingdom aescott2@dstl.gov.uk.Department of Microbiology and Immunology, University of South Alabama, Mobile, Alabama, USA.Defence Science and Technology Laboratory, Porton Down, Salisbury, United Kingdom.Defence Science and Technology Laboratory, Porton Down, Salisbury, United Kingdom.Defence Science and Technology Laboratory, Porton Down, Salisbury, United Kingdom.Defence Science and Technology Laboratory, Porton Down, Salisbury, United Kingdom.Defence Science and Technology Laboratory, Porton Down, Salisbury, United Kingdom.Department of Microbiology and Immunology, University of South Alabama, Mobile, Alabama, USA.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24866807

Citation

Scott, Andrew E., et al. "Burkholderia Pseudomallei Capsular Polysaccharide Conjugates Provide Protection Against Acute Melioidosis." Infection and Immunity, vol. 82, no. 8, 2014, pp. 3206-13.
Scott AE, Burtnick MN, Stokes MG, et al. Burkholderia pseudomallei capsular polysaccharide conjugates provide protection against acute melioidosis. Infect Immun. 2014;82(8):3206-13.
Scott, A. E., Burtnick, M. N., Stokes, M. G., Whelan, A. O., Williamson, E. D., Atkins, T. P., Prior, J. L., & Brett, P. J. (2014). Burkholderia pseudomallei capsular polysaccharide conjugates provide protection against acute melioidosis. Infection and Immunity, 82(8), 3206-13. https://doi.org/10.1128/IAI.01847-14
Scott AE, et al. Burkholderia Pseudomallei Capsular Polysaccharide Conjugates Provide Protection Against Acute Melioidosis. Infect Immun. 2014;82(8):3206-13. PubMed PMID: 24866807.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Burkholderia pseudomallei capsular polysaccharide conjugates provide protection against acute melioidosis. AU - Scott,Andrew E, AU - Burtnick,Mary N, AU - Stokes,Margaret G M, AU - Whelan,Adam O, AU - Williamson,E Diane, AU - Atkins,Timothy P, AU - Prior,Joann L, AU - Brett,Paul J, Y1 - 2014/05/27/ PY - 2014/5/29/entrez PY - 2014/5/29/pubmed PY - 2014/9/10/medline SP - 3206 EP - 13 JF - Infection and immunity JO - Infect Immun VL - 82 IS - 8 N2 - Burkholderia pseudomallei, the etiologic agent of melioidosis, is a CDC tier 1 select agent that causes severe disease in both humans and animals. Diagnosis and treatment of melioidosis can be challenging, and in the absence of optimal chemotherapeutic intervention, acute disease is frequently fatal. Melioidosis is an emerging infectious disease for which there are currently no licensed vaccines. Due to the potential malicious use of B. pseudomallei as well as its impact on public health in regions where the disease is endemic, there is significant interest in developing vaccines for immunization against this disease. In the present study, type A O-polysaccharide (OPS) and manno-heptose capsular polysaccharide (CPS) antigens were isolated from nonpathogenic, select-agent-excluded strains of B. pseudomallei and covalently linked to carrier proteins. By using these conjugates (OPS2B1 and CPS2B1, respectively), it was shown that although high-titer IgG responses against the OPS or CPS component of the glycoconjugates could be raised in BALB/c mice, only those animals immunized with CPS2B1 were protected against intraperitoneal challenge with B. pseudomallei. Extending upon these studies, it was also demonstrated that when the mice were immunized with a combination of CPS2B1 and recombinant B. pseudomallei LolC, rather than with CPS2B1 or LolC individually, they exhibited higher survival rates when challenged with a lethal dose of B. pseudomallei. Collectively, these results suggest that CPS-based glycoconjugates are promising candidates for the development of subunit vaccines for immunization against melioidosis. SN - 1098-5522 UR - https://www.unboundmedicine.com/medline/citation/24866807/Burkholderia_pseudomallei_capsular_polysaccharide_conjugates_provide_protection_against_acute_melioidosis_ L2 - https://journals.asm.org/doi/10.1128/IAI.01847-14?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -