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Stable isotope dilution gas chromatography-mass spectrometry for quantification of thymoquinone in black cumin seed oil.
J Agric Food Chem. 2014 Jun 18; 62(24):5466-71.JA

Abstract

Black cumin seed (Nigella sativa L.) is a widely used spice and herb, where thymoquinone (2-isopropyl-5-methyl-1,4-benzoquinone) is the major bioactive compound. Here, a stable isotope dilution (SID) gas chromatography-mass spectrometry (GC-MS) technique was developed for the quantification of thymoquinone. A doubly deuterated thymoquinone ([(2)H2]-thymoquinone) was synthesized for the first time with more than 93% deuteration degree shown by mass spectrometry and proton nuclear magnetic resonance ((1)H NMR). This compound was used as an internal standard for the quantification of thymoquinone using a SID GC-MS method. The validation experiment showed a recovery rate of 99.1 ± 1.1% relative standard deviation (RSD). Standard addition and external calibration methods have also been used to quantify thymoquinone, which cross-validated the developed stable isotope dilution assay (SIDA). In comparison to external calibration and standard addition methods, the SIDA method is robust and accurate. The concentration of thymoquinone in five marketed black cumin seed oils ranged between 3.34 and 10.8 mg/mL by use of SID GC-MS.

Authors+Show Affiliations

Guy Hilton Research Centre, Institute for Science and Technology in Medicine, Keele University , Thornburrow Drive, ST4 7QB Stoke-on-Trent, United Kingdom.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24871868

Citation

Johnson-Ajinwo, Okiemute Rosa, and Wen-Wu Li. "Stable Isotope Dilution Gas Chromatography-mass Spectrometry for Quantification of Thymoquinone in Black Cumin Seed Oil." Journal of Agricultural and Food Chemistry, vol. 62, no. 24, 2014, pp. 5466-71.
Johnson-Ajinwo OR, Li WW. Stable isotope dilution gas chromatography-mass spectrometry for quantification of thymoquinone in black cumin seed oil. J Agric Food Chem. 2014;62(24):5466-71.
Johnson-Ajinwo, O. R., & Li, W. W. (2014). Stable isotope dilution gas chromatography-mass spectrometry for quantification of thymoquinone in black cumin seed oil. Journal of Agricultural and Food Chemistry, 62(24), 5466-71. https://doi.org/10.1021/jf500357x
Johnson-Ajinwo OR, Li WW. Stable Isotope Dilution Gas Chromatography-mass Spectrometry for Quantification of Thymoquinone in Black Cumin Seed Oil. J Agric Food Chem. 2014 Jun 18;62(24):5466-71. PubMed PMID: 24871868.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Stable isotope dilution gas chromatography-mass spectrometry for quantification of thymoquinone in black cumin seed oil. AU - Johnson-Ajinwo,Okiemute Rosa, AU - Li,Wen-Wu, Y1 - 2014/06/05/ PY - 2014/5/30/entrez PY - 2014/5/30/pubmed PY - 2015/4/14/medline SP - 5466 EP - 71 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 62 IS - 24 N2 - Black cumin seed (Nigella sativa L.) is a widely used spice and herb, where thymoquinone (2-isopropyl-5-methyl-1,4-benzoquinone) is the major bioactive compound. Here, a stable isotope dilution (SID) gas chromatography-mass spectrometry (GC-MS) technique was developed for the quantification of thymoquinone. A doubly deuterated thymoquinone ([(2)H2]-thymoquinone) was synthesized for the first time with more than 93% deuteration degree shown by mass spectrometry and proton nuclear magnetic resonance ((1)H NMR). This compound was used as an internal standard for the quantification of thymoquinone using a SID GC-MS method. The validation experiment showed a recovery rate of 99.1 ± 1.1% relative standard deviation (RSD). Standard addition and external calibration methods have also been used to quantify thymoquinone, which cross-validated the developed stable isotope dilution assay (SIDA). In comparison to external calibration and standard addition methods, the SIDA method is robust and accurate. The concentration of thymoquinone in five marketed black cumin seed oils ranged between 3.34 and 10.8 mg/mL by use of SID GC-MS. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/24871868/Stable_isotope_dilution_gas_chromatography_mass_spectrometry_for_quantification_of_thymoquinone_in_black_cumin_seed_oil_ L2 - https://doi.org/10.1021/jf500357x DB - PRIME DP - Unbound Medicine ER -