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Dextran-coated gold nanoprobes for the concentration and detection of protein biomarkers.
Ann Biomed Eng. 2014 Nov; 42(11):2322-32.AB

Abstract

The lateral-flow immunoassay (LFA) is a well-established point-of-care detection assay that is rapid, inexpensive, easy to use, and portable. However, its sensitivity is lower than that of traditional lab-based assays. Previously, we improved the sensitivity of LFA by concentrating the target biomolecules using aqueous two-phase systems (ATPSs) prior to their detection. In this study, we report the first-ever utilization of dextran-coated gold nanoprobes (DGNPs) as the colorimetric indicator for LFA. In addition, the DGNPs are the key component in our pre-concentration process, where they remain stable and functional in the high salt environment of our ATPS solution, capture the target protein with conjugated antibodies, and allow the rapid concentration of the target protein in our ATPS for use in the subsequent LFA detection step. By combining this pre-concentration step with LFA, the detection limit of LFA for a model protein was improved by 10-fold. We further improved our ATPS from previous studies by enabling phase separation at room temperature in 30 min. By using DGNPs for the concentration and detection of protein biomarkers in the sequential combination of the ATPS and LFA steps, we move closer to developing an effective protein detection assay which uses no power or lab-based equipment.

Authors+Show Affiliations

Department of Bioengineering, University of California, 5121 Engineering V, 420 Westwood Plaza, Los Angeles, CA, 90095-1600, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24874602

Citation

Chiu, Ricky Y T., et al. "Dextran-coated Gold Nanoprobes for the Concentration and Detection of Protein Biomarkers." Annals of Biomedical Engineering, vol. 42, no. 11, 2014, pp. 2322-32.
Chiu RY, Nguyen PT, Wang J, et al. Dextran-coated gold nanoprobes for the concentration and detection of protein biomarkers. Ann Biomed Eng. 2014;42(11):2322-32.
Chiu, R. Y., Nguyen, P. T., Wang, J., Jue, E., Wu, B. M., & Kamei, D. T. (2014). Dextran-coated gold nanoprobes for the concentration and detection of protein biomarkers. Annals of Biomedical Engineering, 42(11), 2322-32. https://doi.org/10.1007/s10439-014-1043-3
Chiu RY, et al. Dextran-coated Gold Nanoprobes for the Concentration and Detection of Protein Biomarkers. Ann Biomed Eng. 2014;42(11):2322-32. PubMed PMID: 24874602.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dextran-coated gold nanoprobes for the concentration and detection of protein biomarkers. AU - Chiu,Ricky Y T, AU - Nguyen,Phuong T, AU - Wang,Juntian, AU - Jue,Erik, AU - Wu,Benjamin M, AU - Kamei,Daniel T, Y1 - 2014/05/30/ PY - 2014/02/28/received PY - 2014/05/22/accepted PY - 2014/5/31/entrez PY - 2014/5/31/pubmed PY - 2015/6/24/medline SP - 2322 EP - 32 JF - Annals of biomedical engineering JO - Ann Biomed Eng VL - 42 IS - 11 N2 - The lateral-flow immunoassay (LFA) is a well-established point-of-care detection assay that is rapid, inexpensive, easy to use, and portable. However, its sensitivity is lower than that of traditional lab-based assays. Previously, we improved the sensitivity of LFA by concentrating the target biomolecules using aqueous two-phase systems (ATPSs) prior to their detection. In this study, we report the first-ever utilization of dextran-coated gold nanoprobes (DGNPs) as the colorimetric indicator for LFA. In addition, the DGNPs are the key component in our pre-concentration process, where they remain stable and functional in the high salt environment of our ATPS solution, capture the target protein with conjugated antibodies, and allow the rapid concentration of the target protein in our ATPS for use in the subsequent LFA detection step. By combining this pre-concentration step with LFA, the detection limit of LFA for a model protein was improved by 10-fold. We further improved our ATPS from previous studies by enabling phase separation at room temperature in 30 min. By using DGNPs for the concentration and detection of protein biomarkers in the sequential combination of the ATPS and LFA steps, we move closer to developing an effective protein detection assay which uses no power or lab-based equipment. SN - 1573-9686 UR - https://www.unboundmedicine.com/medline/citation/24874602/Dextran_coated_gold_nanoprobes_for_the_concentration_and_detection_of_protein_biomarkers_ L2 - https://doi.org/10.1007/s10439-014-1043-3 DB - PRIME DP - Unbound Medicine ER -