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The role of ceramide chain length distribution on the barrier properties of the skin lipid membranes.
Biochim Biophys Acta. 2014 Oct; 1838(10):2473-83.BB

Abstract

The skin barrier function is provided by the stratum corneum (SC). The lipids in the SC are composed of three lipid classes: ceramides (CERs), cholesterol (CHOL) and free fatty acids (FFAs) which form two crystalline lamellar structures. In the present study, we investigate the effect of CER chain length distribution on the barrier properties of model lipid membranes mimicking the lipid composition and organization of SC. The membranes were prepared with either isolated pig CERs (PCERs) or synthetic CERs. While PCERs have a wide chain length distribution, the synthetic CERs are quite uniform in chain length. The barrier properties were examined by means of permeation studies using hydrocortisone as a model drug. Our studies revealed a reduced barrier in lipid membranes prepared with PCERs compared to synthetic CERs. Additional studies revealed that a wider chain length distribution of PCERs results in an enhanced hexagonal packing and increased conformational disordering of the lipid tails compared to synthetic CERs, while the lamellar phases did not change. This demonstrates that the chain length distribution affects the lipid barrier by reducing the lipid ordering and density within the lipid lamellae. In subsequent studies, the effect of increased levels of FFAs or CERs with a long acyl chain in the PCERs membranes was also studied. These changes in lipid composition enhanced the level of orthorhombic packing, reduced the conformational disordering and increased the barrier of the lipid membranes. In conclusion, the CER chain length distribution is an important key factor for maintaining a proper barrier.

Authors+Show Affiliations

Leiden Academic Center for Drug Research, Department of Drug Delivery Technology, Gorlaeus Laboratories, University of Leiden, Leiden, the Netherlands.Leiden Academic Center for Drug Research, Department of Drug Delivery Technology, Gorlaeus Laboratories, University of Leiden, Leiden, the Netherlands.Leiden Academic Center for Drug Research, Department of Drug Delivery Technology, Gorlaeus Laboratories, University of Leiden, Leiden, the Netherlands.Leiden Academic Center for Drug Research, Department of Drug Delivery Technology, Gorlaeus Laboratories, University of Leiden, Leiden, the Netherlands.Leiden Academic Center for Drug Research, Department of Drug Delivery Technology, Gorlaeus Laboratories, University of Leiden, Leiden, the Netherlands. Electronic address: bouwstra@chem.leidenuniv.nl.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

24875266

Citation

Mojumdar, E H., et al. "The Role of Ceramide Chain Length Distribution On the Barrier Properties of the Skin Lipid Membranes." Biochimica Et Biophysica Acta, vol. 1838, no. 10, 2014, pp. 2473-83.
Mojumdar EH, Kariman Z, van Kerckhove L, et al. The role of ceramide chain length distribution on the barrier properties of the skin lipid membranes. Biochim Biophys Acta. 2014;1838(10):2473-83.
Mojumdar, E. H., Kariman, Z., van Kerckhove, L., Gooris, G. S., & Bouwstra, J. A. (2014). The role of ceramide chain length distribution on the barrier properties of the skin lipid membranes. Biochimica Et Biophysica Acta, 1838(10), 2473-83. https://doi.org/10.1016/j.bbamem.2014.05.023
Mojumdar EH, et al. The Role of Ceramide Chain Length Distribution On the Barrier Properties of the Skin Lipid Membranes. Biochim Biophys Acta. 2014;1838(10):2473-83. PubMed PMID: 24875266.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The role of ceramide chain length distribution on the barrier properties of the skin lipid membranes. AU - Mojumdar,E H, AU - Kariman,Z, AU - van Kerckhove,L, AU - Gooris,G S, AU - Bouwstra,J A, Y1 - 2014/05/27/ PY - 2013/12/06/received PY - 2014/04/25/revised PY - 2014/05/19/accepted PY - 2014/5/31/entrez PY - 2014/5/31/pubmed PY - 2014/10/2/medline KW - Infrared spectroscopy KW - Lipid mixtures KW - Pig ceramides KW - Stratum corneum KW - X-ray diffraction SP - 2473 EP - 83 JF - Biochimica et biophysica acta JO - Biochim Biophys Acta VL - 1838 IS - 10 N2 - The skin barrier function is provided by the stratum corneum (SC). The lipids in the SC are composed of three lipid classes: ceramides (CERs), cholesterol (CHOL) and free fatty acids (FFAs) which form two crystalline lamellar structures. In the present study, we investigate the effect of CER chain length distribution on the barrier properties of model lipid membranes mimicking the lipid composition and organization of SC. The membranes were prepared with either isolated pig CERs (PCERs) or synthetic CERs. While PCERs have a wide chain length distribution, the synthetic CERs are quite uniform in chain length. The barrier properties were examined by means of permeation studies using hydrocortisone as a model drug. Our studies revealed a reduced barrier in lipid membranes prepared with PCERs compared to synthetic CERs. Additional studies revealed that a wider chain length distribution of PCERs results in an enhanced hexagonal packing and increased conformational disordering of the lipid tails compared to synthetic CERs, while the lamellar phases did not change. This demonstrates that the chain length distribution affects the lipid barrier by reducing the lipid ordering and density within the lipid lamellae. In subsequent studies, the effect of increased levels of FFAs or CERs with a long acyl chain in the PCERs membranes was also studied. These changes in lipid composition enhanced the level of orthorhombic packing, reduced the conformational disordering and increased the barrier of the lipid membranes. In conclusion, the CER chain length distribution is an important key factor for maintaining a proper barrier. SN - 0006-3002 UR - https://www.unboundmedicine.com/medline/citation/24875266/The_role_of_ceramide_chain_length_distribution_on_the_barrier_properties_of_the_skin_lipid_membranes_ DB - PRIME DP - Unbound Medicine ER -