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Allele and haplotype frequencies for HLA-DQ in Iranian celiac disease patients.
World J Gastroenterol. 2014 May 28; 20(20):6302-8.WJ

Abstract

AIM

To assess the distribution of human leukocyte antigen (HLA)-DQ2 and -DQ8 in Iranian celiac disease (CD) patients and compare them to healthy Iranian controls.

METHODS

To predict the HLA-DQA1 and -DQB1 genes, we used six previously reported HLA-tagging single nucleotide polymorphism to determine HLA genotypes in 59 Iranian patients with 'biopsy-confirmed' CD and in 151 healthy Iranian individuals. To test the transferability of the method, 50 cases and controls were also typed using a commercial kit that identifies individual carriers of DQ2, DQ7 and DQ8 alleles.

RESULTS

In this pilot study 97% of CD cases (n = 57) and 58% of controls (n = 87) were carriers of HLA-DQ2 and/or HLA-DQ8 heterodimers, either in the homozygous or heterozygous state. The HLA-DQ pattern of these 57 CD patients: heterozygous DQ2.2 (n = 14) and homozygous DQ2.2 (n = 1), heterozygous DQ2.5 (n = 33) and homozygous DQ2.5 (n = 8), heterozygous DQ8 (n = 13) and homozygous DQ8 (n = 2). Two CD patients were negative for both DQ2 and DQ8 (3%).

CONCLUSION

The prevalence of DQ8 in our CD population was higher than that reported in other populations (25.4%). As reported in other populations, our results underline the primary importance of HLA-DQ alleles in the Iranian population's susceptibility to CD.

Authors+Show Affiliations

Mohammad Rostami-Nejad, Mohammad Javad Ehsani-Ardakani, Homayoun Zojaji, Seyed Reza Mohebbi, Mohammad-Reza Zali, Department of Celiac Disease, Gastroenterology and Liver Diseases Research Centers, Shahid Beheshti University of Medical Sciences, Tehran 1985717411, Iran.Mohammad Rostami-Nejad, Mohammad Javad Ehsani-Ardakani, Homayoun Zojaji, Seyed Reza Mohebbi, Mohammad-Reza Zali, Department of Celiac Disease, Gastroenterology and Liver Diseases Research Centers, Shahid Beheshti University of Medical Sciences, Tehran 1985717411, Iran.Mohammad Rostami-Nejad, Mohammad Javad Ehsani-Ardakani, Homayoun Zojaji, Seyed Reza Mohebbi, Mohammad-Reza Zali, Department of Celiac Disease, Gastroenterology and Liver Diseases Research Centers, Shahid Beheshti University of Medical Sciences, Tehran 1985717411, Iran.Mohammad Rostami-Nejad, Mohammad Javad Ehsani-Ardakani, Homayoun Zojaji, Seyed Reza Mohebbi, Mohammad-Reza Zali, Department of Celiac Disease, Gastroenterology and Liver Diseases Research Centers, Shahid Beheshti University of Medical Sciences, Tehran 1985717411, Iran.Mohammad Rostami-Nejad, Mohammad Javad Ehsani-Ardakani, Homayoun Zojaji, Seyed Reza Mohebbi, Mohammad-Reza Zali, Department of Celiac Disease, Gastroenterology and Liver Diseases Research Centers, Shahid Beheshti University of Medical Sciences, Tehran 1985717411, Iran.Mohammad Rostami-Nejad, Mohammad Javad Ehsani-Ardakani, Homayoun Zojaji, Seyed Reza Mohebbi, Mohammad-Reza Zali, Department of Celiac Disease, Gastroenterology and Liver Diseases Research Centers, Shahid Beheshti University of Medical Sciences, Tehran 1985717411, Iran.Mohammad Rostami-Nejad, Mohammad Javad Ehsani-Ardakani, Homayoun Zojaji, Seyed Reza Mohebbi, Mohammad-Reza Zali, Department of Celiac Disease, Gastroenterology and Liver Diseases Research Centers, Shahid Beheshti University of Medical Sciences, Tehran 1985717411, Iran.Mohammad Rostami-Nejad, Mohammad Javad Ehsani-Ardakani, Homayoun Zojaji, Seyed Reza Mohebbi, Mohammad-Reza Zali, Department of Celiac Disease, Gastroenterology and Liver Diseases Research Centers, Shahid Beheshti University of Medical Sciences, Tehran 1985717411, Iran.Mohammad Rostami-Nejad, Mohammad Javad Ehsani-Ardakani, Homayoun Zojaji, Seyed Reza Mohebbi, Mohammad-Reza Zali, Department of Celiac Disease, Gastroenterology and Liver Diseases Research Centers, Shahid Beheshti University of Medical Sciences, Tehran 1985717411, Iran.Mohammad Rostami-Nejad, Mohammad Javad Ehsani-Ardakani, Homayoun Zojaji, Seyed Reza Mohebbi, Mohammad-Reza Zali, Department of Celiac Disease, Gastroenterology and Liver Diseases Research Centers, Shahid Beheshti University of Medical Sciences, Tehran 1985717411, Iran.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24876751

Citation

Rostami-Nejad, Mohammad, et al. "Allele and Haplotype Frequencies for HLA-DQ in Iranian Celiac Disease Patients." World Journal of Gastroenterology, vol. 20, no. 20, 2014, pp. 6302-8.
Rostami-Nejad M, Romanos J, Rostami K, et al. Allele and haplotype frequencies for HLA-DQ in Iranian celiac disease patients. World J Gastroenterol. 2014;20(20):6302-8.
Rostami-Nejad, M., Romanos, J., Rostami, K., Ganji, A., Ehsani-Ardakani, M. J., Bakhshipour, A. R., Zojaji, H., Mohebbi, S. R., Zali, M. R., & Wijmenga, C. (2014). Allele and haplotype frequencies for HLA-DQ in Iranian celiac disease patients. World Journal of Gastroenterology, 20(20), 6302-8. https://doi.org/10.3748/wjg.v20.i20.6302
Rostami-Nejad M, et al. Allele and Haplotype Frequencies for HLA-DQ in Iranian Celiac Disease Patients. World J Gastroenterol. 2014 May 28;20(20):6302-8. PubMed PMID: 24876751.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Allele and haplotype frequencies for HLA-DQ in Iranian celiac disease patients. AU - Rostami-Nejad,Mohammad, AU - Romanos,Jihane, AU - Rostami,Kamran, AU - Ganji,Azita, AU - Ehsani-Ardakani,Mohammad Javad, AU - Bakhshipour,Ali-Reza, AU - Zojaji,Homayoun, AU - Mohebbi,Seyed Reza, AU - Zali,Mohammad-Reza, AU - Wijmenga,Cisca, PY - 2013/11/02/received PY - 2013/12/31/revised PY - 2014/01/20/accepted PY - 2014/5/31/entrez PY - 2014/5/31/pubmed PY - 2015/4/14/medline KW - Celiac disease KW - Human leukocyte antigen typing KW - Iran KW - Susceptibility KW - Validation SP - 6302 EP - 8 JF - World journal of gastroenterology JO - World J. Gastroenterol. VL - 20 IS - 20 N2 - AIM: To assess the distribution of human leukocyte antigen (HLA)-DQ2 and -DQ8 in Iranian celiac disease (CD) patients and compare them to healthy Iranian controls. METHODS: To predict the HLA-DQA1 and -DQB1 genes, we used six previously reported HLA-tagging single nucleotide polymorphism to determine HLA genotypes in 59 Iranian patients with 'biopsy-confirmed' CD and in 151 healthy Iranian individuals. To test the transferability of the method, 50 cases and controls were also typed using a commercial kit that identifies individual carriers of DQ2, DQ7 and DQ8 alleles. RESULTS: In this pilot study 97% of CD cases (n = 57) and 58% of controls (n = 87) were carriers of HLA-DQ2 and/or HLA-DQ8 heterodimers, either in the homozygous or heterozygous state. The HLA-DQ pattern of these 57 CD patients: heterozygous DQ2.2 (n = 14) and homozygous DQ2.2 (n = 1), heterozygous DQ2.5 (n = 33) and homozygous DQ2.5 (n = 8), heterozygous DQ8 (n = 13) and homozygous DQ8 (n = 2). Two CD patients were negative for both DQ2 and DQ8 (3%). CONCLUSION: The prevalence of DQ8 in our CD population was higher than that reported in other populations (25.4%). As reported in other populations, our results underline the primary importance of HLA-DQ alleles in the Iranian population's susceptibility to CD. SN - 2219-2840 UR - https://www.unboundmedicine.com/medline/citation/24876751/Allele_and_haplotype_frequencies_for_HLA_DQ_in_Iranian_celiac_disease_patients_ L2 - http://www.wjgnet.com/1007-9327/full/v20/i20/6302.htm DB - PRIME DP - Unbound Medicine ER -