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Ciguatera fish poisoning in Hong Kong--a 10-year perspective on the class of ciguatoxins.
Toxicon. 2014 Aug; 86:96-106.T

Abstract

The present study used liquid chromatography-tandem mass spectrometry (LC-MS/MS) to investigate retrospectively ciguatoxin (CTX)-positive samples as determined by mouse bioassay (MBA) in the past 10 years in Hong Kong. The results showed that Pacific CTXs (P-CTX-1, -2 and -3) were the most commonly observed toxins found in the samples, indicating Pacific Ocean areas as the most important origin of ciguatera fish poisoning. Clinical diagnosis from ciguatera patients also revealed the predominance of neurological illnesses in most cases, supporting intoxication of Pacific origin. This study demonstrated the ability of laboratory analysis to identify and quantify Pacific CTXs in suspected fish samples, so as to support the clinical diagnosis of ciguatera. Comparative analysis (Student's t-test and Spearman's rank correlation analysis) on the two CTX detection methods showed approximate linearity for overall P-CTXs (P-CTX-1, -2 and -3)/P-CTX-1 alone as derived by LC-MS/MS and total toxicity levels (P-CTX-1 equivalent) as determined by MBA. The LC-MS/MS method coupled with the rapid extraction method could allow the detection of trace amount of CTXs at levels below the clinically relevant limit, 0.1 ppb P-CTX-1 in fish flesh. For practical application, the adoption of a two-tiered approach for testing, chemical analysis by LC-MS/MS for toxic fish screening, coupled with biological assay by MBA for final toxicity confirmation, was proposed for first-line screening of CTX in potentially contaminated fish samples in the market, with an aim to minimizing the use of laboratory mice and at the same time providing reasonably effective means for routine analysis.

Authors+Show Affiliations

Public Health Laboratory Services Branch, Centre for Health Protection, Department of Health, 382 Nam Cheong St., Shek Kip Mei, Kowloon, Hong Kong, China. Electronic address: so_phls5@dh.gov.hk.Public Health Laboratory Services Branch, Centre for Health Protection, Department of Health, 382 Nam Cheong St., Shek Kip Mei, Kowloon, Hong Kong, China. Electronic address: so_phls2@dh.gov.hk.Public Health Laboratory Services Branch, Centre for Health Protection, Department of Health, 382 Nam Cheong St., Shek Kip Mei, Kowloon, Hong Kong, China. Electronic address: janicelo@dh.gov.hk.

Pub Type(s)

Journal Article
Validation Study

Language

eng

PubMed ID

24878373

Citation

Wong, Chun-Kwan, et al. "Ciguatera Fish Poisoning in Hong Kong--a 10-year Perspective On the Class of Ciguatoxins." Toxicon : Official Journal of the International Society On Toxinology, vol. 86, 2014, pp. 96-106.
Wong CK, Hung P, Lo JY. Ciguatera fish poisoning in Hong Kong--a 10-year perspective on the class of ciguatoxins. Toxicon. 2014;86:96-106.
Wong, C. K., Hung, P., & Lo, J. Y. (2014). Ciguatera fish poisoning in Hong Kong--a 10-year perspective on the class of ciguatoxins. Toxicon : Official Journal of the International Society On Toxinology, 86, 96-106. https://doi.org/10.1016/j.toxicon.2014.05.006
Wong CK, Hung P, Lo JY. Ciguatera Fish Poisoning in Hong Kong--a 10-year Perspective On the Class of Ciguatoxins. Toxicon. 2014;86:96-106. PubMed PMID: 24878373.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ciguatera fish poisoning in Hong Kong--a 10-year perspective on the class of ciguatoxins. AU - Wong,Chun-Kwan, AU - Hung,Patricia, AU - Lo,Janice Y C, Y1 - 2014/05/28/ PY - 2014/01/17/received PY - 2014/05/13/revised PY - 2014/05/16/accepted PY - 2014/6/1/entrez PY - 2014/6/1/pubmed PY - 2015/2/24/medline KW - Ciguatera fish poisoning KW - Epidemiology KW - LC-MS/MS KW - Pacific CTX KW - Public health SP - 96 EP - 106 JF - Toxicon : official journal of the International Society on Toxinology JO - Toxicon VL - 86 N2 - The present study used liquid chromatography-tandem mass spectrometry (LC-MS/MS) to investigate retrospectively ciguatoxin (CTX)-positive samples as determined by mouse bioassay (MBA) in the past 10 years in Hong Kong. The results showed that Pacific CTXs (P-CTX-1, -2 and -3) were the most commonly observed toxins found in the samples, indicating Pacific Ocean areas as the most important origin of ciguatera fish poisoning. Clinical diagnosis from ciguatera patients also revealed the predominance of neurological illnesses in most cases, supporting intoxication of Pacific origin. This study demonstrated the ability of laboratory analysis to identify and quantify Pacific CTXs in suspected fish samples, so as to support the clinical diagnosis of ciguatera. Comparative analysis (Student's t-test and Spearman's rank correlation analysis) on the two CTX detection methods showed approximate linearity for overall P-CTXs (P-CTX-1, -2 and -3)/P-CTX-1 alone as derived by LC-MS/MS and total toxicity levels (P-CTX-1 equivalent) as determined by MBA. The LC-MS/MS method coupled with the rapid extraction method could allow the detection of trace amount of CTXs at levels below the clinically relevant limit, 0.1 ppb P-CTX-1 in fish flesh. For practical application, the adoption of a two-tiered approach for testing, chemical analysis by LC-MS/MS for toxic fish screening, coupled with biological assay by MBA for final toxicity confirmation, was proposed for first-line screening of CTX in potentially contaminated fish samples in the market, with an aim to minimizing the use of laboratory mice and at the same time providing reasonably effective means for routine analysis. SN - 1879-3150 UR - https://www.unboundmedicine.com/medline/citation/24878373/Ciguatera_fish_poisoning_in_Hong_Kong__a_10_year_perspective_on_the_class_of_ciguatoxins_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0041-0101(14)00132-9 DB - PRIME DP - Unbound Medicine ER -