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Effect of the timing of posthatch feed restrictions on broiler breast muscle development and muscle transcriptional regulatory factor gene expression.
Poult Sci. 2014 Jun; 93(6):1484-94.PS

Abstract

The effect of the timing of an immediate posthatch feed restriction on broiler pectoralis major muscle development was studied by applying a 20% feed restriction either the first or second week after hatch. Pectoralis major muscle morphological structure and the expression of the myogenic transcriptional regulatory factors, myogenic determination factor 1 (MyoD), myogenic regulatory factor 4 (MRF4), and myogenin, were measured. Broiler chicks at hatch were divided into a full-fed (control) group and a 20% feed restriction treatment administered either the first or second week posthatch. At the end of the feed restriction, the chicks were placed on a full feed ad libitum diet with no further restrictions. Muscle fiber diameter and fiber bundle size of the pectoralis major muscle were smaller in the wk 1 restricted group than the control group by 7 d of age. By 15 d of age through the duration of the study, d 43, both endomysial and perimysial connective tissue spacing were diminished in the wk 1 feed-restricted group. The expression of MyoD, MRF4, and myogenin was affected by the wk 1 feed restriction. The expression of MyoD and MRF4 was significantly increased during the first week posthatch. Both of the genes have been shown to be expressed during proliferation especially MyoD, which is required for muscle cell proliferation. In contrast, myogenin expression was significantly decreased. Myogenin expression is required for differentiation to occur. The morphological changes and gene expression changes observed with the wk 1 feed restriction were eliminated by moving the 20% feed restriction to wk 2, which is after the period of maximal myogenic satellite cell mitotic activity. Taken together, these results demonstrate that the timing of early posthatch feed restrictions to chicks is critical for the morphological development of the pectoralis major muscle and the expression of genes required for muscle satellite cell proliferation and differentiation.

Authors+Show Affiliations

Department of Animal Sciences, Ohio Agricultural Research and Development Center, The Ohio State University, 1680 Madison Ave, Wooster 44691 Velleman.1@osu.edu.Department of Animal Sciences, Ohio Agricultural Research and Development Center, The Ohio State University, 1680 Madison Ave, Wooster 44691.Aviagen Incorporated, 5015 Bradford Dr, Huntsville, AL 35805.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24879698

Citation

Velleman, S G., et al. "Effect of the Timing of Posthatch Feed Restrictions On Broiler Breast Muscle Development and Muscle Transcriptional Regulatory Factor Gene Expression." Poultry Science, vol. 93, no. 6, 2014, pp. 1484-94.
Velleman SG, Coy CS, Emmerson DA. Effect of the timing of posthatch feed restrictions on broiler breast muscle development and muscle transcriptional regulatory factor gene expression. Poult Sci. 2014;93(6):1484-94.
Velleman, S. G., Coy, C. S., & Emmerson, D. A. (2014). Effect of the timing of posthatch feed restrictions on broiler breast muscle development and muscle transcriptional regulatory factor gene expression. Poultry Science, 93(6), 1484-94. https://doi.org/10.3382/ps.2013-03813
Velleman SG, Coy CS, Emmerson DA. Effect of the Timing of Posthatch Feed Restrictions On Broiler Breast Muscle Development and Muscle Transcriptional Regulatory Factor Gene Expression. Poult Sci. 2014;93(6):1484-94. PubMed PMID: 24879698.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of the timing of posthatch feed restrictions on broiler breast muscle development and muscle transcriptional regulatory factor gene expression. AU - Velleman,S G, AU - Coy,C S, AU - Emmerson,D A, PY - 2014/6/1/entrez PY - 2014/6/1/pubmed PY - 2014/7/18/medline KW - chick KW - feed restriction KW - muscle KW - muscle-specific transcriptional regulatory factor KW - satellite cell SP - 1484 EP - 94 JF - Poultry science JO - Poult Sci VL - 93 IS - 6 N2 - The effect of the timing of an immediate posthatch feed restriction on broiler pectoralis major muscle development was studied by applying a 20% feed restriction either the first or second week after hatch. Pectoralis major muscle morphological structure and the expression of the myogenic transcriptional regulatory factors, myogenic determination factor 1 (MyoD), myogenic regulatory factor 4 (MRF4), and myogenin, were measured. Broiler chicks at hatch were divided into a full-fed (control) group and a 20% feed restriction treatment administered either the first or second week posthatch. At the end of the feed restriction, the chicks were placed on a full feed ad libitum diet with no further restrictions. Muscle fiber diameter and fiber bundle size of the pectoralis major muscle were smaller in the wk 1 restricted group than the control group by 7 d of age. By 15 d of age through the duration of the study, d 43, both endomysial and perimysial connective tissue spacing were diminished in the wk 1 feed-restricted group. The expression of MyoD, MRF4, and myogenin was affected by the wk 1 feed restriction. The expression of MyoD and MRF4 was significantly increased during the first week posthatch. Both of the genes have been shown to be expressed during proliferation especially MyoD, which is required for muscle cell proliferation. In contrast, myogenin expression was significantly decreased. Myogenin expression is required for differentiation to occur. The morphological changes and gene expression changes observed with the wk 1 feed restriction were eliminated by moving the 20% feed restriction to wk 2, which is after the period of maximal myogenic satellite cell mitotic activity. Taken together, these results demonstrate that the timing of early posthatch feed restrictions to chicks is critical for the morphological development of the pectoralis major muscle and the expression of genes required for muscle satellite cell proliferation and differentiation. SN - 0032-5791 UR - https://www.unboundmedicine.com/medline/citation/24879698/Effect_of_the_timing_of_posthatch_feed_restrictions_on_broiler_breast_muscle_development_and_muscle_transcriptional_regulatory_factor_gene_expression_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0032-5791(19)32321-1 DB - PRIME DP - Unbound Medicine ER -