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Identification of G protein-coupled receptors in Schistosoma haematobium and S. mansoni by comparative genomics.
Parasit Vectors 2014; 7:242PV

Abstract

BACKGROUND

Schistosomiasis is a parasitic disease affecting ~200 million people worldwide. Schistosoma haematobium and S. mansoni are two relatively closely related schistosomes (blood flukes), and the causative agents of urogenital and hepatointestinal schistosomiasis, respectively. The availability of genomic, transcriptomic and proteomic data sets for these two schistosomes now provides unprecedented opportunities to explore their biology, host interactions and schistosomiasis at the molecular level. A particularly important group of molecules involved in a range of biological and developmental processes in schistosomes and other parasites are the G protein-coupled receptors (GPCRs). Although GPCRs have been studied in schistosomes, there has been no detailed comparison of these receptors between closely related species. Here, using a genomic-bioinformatic approach, we identified and characterised key GPCRs in S. haematobium and S. mansoni (two closely related species of schistosome).

METHODS

Using a Hidden Markov Model (HMM) and Support Vector Machine (SVM)-based pipeline, we classified and sub-classified GPCRs of S. haematobium and S. mansoni, combined with phylogenetic and transcription analyses.

RESULTS

We identified and classified classes A, B, C and F as well as an unclassified group of GPCRs encoded in the genomes of S. haematobium and S. mansoni. In addition, we characterised ligand-specific subclasses (i.e. amine, peptide, opsin and orphan) within class A (rhodopsin-like).

CONCLUSIONS

Most GPCRs shared a high degree of similarity and conservation, except for members of a particular clade (designated SmGPR), which appear to have diverged between S. haematobium and S. mansoni and might explain, to some extent, some of the underlying biological differences between these two schistosomes. The present set of annotated GPCRs provides a basis for future functional genomic studies of cellular GPCR-mediated signal transduction and a resource for future drug discovery efforts in schistosomes.

Authors+Show Affiliations

No affiliation info availableFaculty of Veterinary Science, The University of Melbourne, Parkville, Victoria, Australia. nyoung@unimelb.edu.au.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24884876

Citation

Campos, Tulio D L., et al. "Identification of G Protein-coupled Receptors in Schistosoma Haematobium and S. Mansoni By Comparative Genomics." Parasites & Vectors, vol. 7, 2014, p. 242.
Campos TD, Young ND, Korhonen PK, et al. Identification of G protein-coupled receptors in Schistosoma haematobium and S. mansoni by comparative genomics. Parasit Vectors. 2014;7:242.
Campos, T. D., Young, N. D., Korhonen, P. K., Hall, R. S., Mangiola, S., Lonie, A., & Gasser, R. B. (2014). Identification of G protein-coupled receptors in Schistosoma haematobium and S. mansoni by comparative genomics. Parasites & Vectors, 7, p. 242. doi:10.1186/1756-3305-7-242.
Campos TD, et al. Identification of G Protein-coupled Receptors in Schistosoma Haematobium and S. Mansoni By Comparative Genomics. Parasit Vectors. 2014 May 27;7:242. PubMed PMID: 24884876.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of G protein-coupled receptors in Schistosoma haematobium and S. mansoni by comparative genomics. AU - Campos,Tulio D L, AU - Young,Neil D, AU - Korhonen,Pasi K, AU - Hall,Ross S, AU - Mangiola,Stefano, AU - Lonie,Andrew, AU - Gasser,Robin B, Y1 - 2014/05/27/ PY - 2014/05/08/received PY - 2014/05/17/accepted PY - 2014/6/3/entrez PY - 2014/6/3/pubmed PY - 2015/2/5/medline SP - 242 EP - 242 JF - Parasites & vectors JO - Parasit Vectors VL - 7 N2 - BACKGROUND: Schistosomiasis is a parasitic disease affecting ~200 million people worldwide. Schistosoma haematobium and S. mansoni are two relatively closely related schistosomes (blood flukes), and the causative agents of urogenital and hepatointestinal schistosomiasis, respectively. The availability of genomic, transcriptomic and proteomic data sets for these two schistosomes now provides unprecedented opportunities to explore their biology, host interactions and schistosomiasis at the molecular level. A particularly important group of molecules involved in a range of biological and developmental processes in schistosomes and other parasites are the G protein-coupled receptors (GPCRs). Although GPCRs have been studied in schistosomes, there has been no detailed comparison of these receptors between closely related species. Here, using a genomic-bioinformatic approach, we identified and characterised key GPCRs in S. haematobium and S. mansoni (two closely related species of schistosome). METHODS: Using a Hidden Markov Model (HMM) and Support Vector Machine (SVM)-based pipeline, we classified and sub-classified GPCRs of S. haematobium and S. mansoni, combined with phylogenetic and transcription analyses. RESULTS: We identified and classified classes A, B, C and F as well as an unclassified group of GPCRs encoded in the genomes of S. haematobium and S. mansoni. In addition, we characterised ligand-specific subclasses (i.e. amine, peptide, opsin and orphan) within class A (rhodopsin-like). CONCLUSIONS: Most GPCRs shared a high degree of similarity and conservation, except for members of a particular clade (designated SmGPR), which appear to have diverged between S. haematobium and S. mansoni and might explain, to some extent, some of the underlying biological differences between these two schistosomes. The present set of annotated GPCRs provides a basis for future functional genomic studies of cellular GPCR-mediated signal transduction and a resource for future drug discovery efforts in schistosomes. SN - 1756-3305 UR - https://www.unboundmedicine.com/medline/citation/24884876/Identification_of_G_protein_coupled_receptors_in_Schistosoma_haematobium_and_S__mansoni_by_comparative_genomics_ L2 - https://parasitesandvectors.biomedcentral.com/articles/10.1186/1756-3305-7-242 DB - PRIME DP - Unbound Medicine ER -