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Samba virus: a novel mimivirus from a giant rain forest, the Brazilian Amazon.
Virol J. 2014 May 14; 11:95.VJ

Abstract

BACKGROUND

The identification of novel giant viruses from the nucleocytoplasmic large DNA viruses group and their virophages has increased in the last decade and has helped to shed light on viral evolution. This study describe the discovery, isolation and characterization of Samba virus (SMBV), a novel giant virus belonging to the Mimivirus genus, which was isolated from the Negro River in the Brazilian Amazon. We also report the isolation of an SMBV-associated virophage named Rio Negro (RNV), which is the first Mimivirus virophage to be isolated in the Americas.

METHODS/RESULTS

Based on a phylogenetic analysis, SMBV belongs to group A of the putative Megavirales order, possibly a new virus related to Acanthamoeba polyphaga mimivirus (APMV). SMBV is the largest virus isolated in Brazil, with an average particle diameter about 574 nm. The SMBV genome contains 938 ORFs, of which nine are ORFans. The 1,213.6 kb SMBV genome is one of the largest genome of any group A Mimivirus described to date. Electron microscopy showed RNV particle accumulation near SMBV and APMV factories resulting in the production of defective SMBV and APMV particles and decreasing the infectivity of these two viruses by several logs.

CONCLUSION

This discovery expands our knowledge of Mimiviridae evolution and ecology.

Authors+Show Affiliations

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableDepartamento de Microbiologia, Universidade Federal de Minas Gerais, Laboratório de Vírus, Av, Antônio Carlos, 6627 Pampulha, Belo Horizonte, MG Zip Code 31270-901, Brazil. bernard.la-scola@univ-amu.fr.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

24886672

Citation

Campos, Rafael K., et al. "Samba Virus: a Novel Mimivirus From a Giant Rain Forest, the Brazilian Amazon." Virology Journal, vol. 11, 2014, p. 95.
Campos RK, Boratto PV, Assis FL, et al. Samba virus: a novel mimivirus from a giant rain forest, the Brazilian Amazon. Virol J. 2014;11:95.
Campos, R. K., Boratto, P. V., Assis, F. L., Aguiar, E. R., Silva, L. C., Albarnaz, J. D., Dornas, F. P., Trindade, G. S., Ferreira, P. P., Marques, J. T., Robert, C., Raoult, D., Kroon, E. G., La Scola, B., & Abrahão, J. S. (2014). Samba virus: a novel mimivirus from a giant rain forest, the Brazilian Amazon. Virology Journal, 11, 95. https://doi.org/10.1186/1743-422X-11-95
Campos RK, et al. Samba Virus: a Novel Mimivirus From a Giant Rain Forest, the Brazilian Amazon. Virol J. 2014 May 14;11:95. PubMed PMID: 24886672.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Samba virus: a novel mimivirus from a giant rain forest, the Brazilian Amazon. AU - Campos,Rafael K, AU - Boratto,Paulo V, AU - Assis,Felipe L, AU - Aguiar,Eric R G R, AU - Silva,Lorena C F, AU - Albarnaz,Jonas D, AU - Dornas,Fabio P, AU - Trindade,Giliane S, AU - Ferreira,Paulo P, AU - Marques,João T, AU - Robert,Catherine, AU - Raoult,Didier, AU - Kroon,Erna G, AU - La Scola,Bernard, AU - Abrahão,Jônatas S, Y1 - 2014/05/14/ PY - 2014/02/19/received PY - 2014/05/01/accepted PY - 2014/6/3/entrez PY - 2014/6/3/pubmed PY - 2015/1/27/medline SP - 95 EP - 95 JF - Virology journal JO - Virol. J. VL - 11 N2 - BACKGROUND: The identification of novel giant viruses from the nucleocytoplasmic large DNA viruses group and their virophages has increased in the last decade and has helped to shed light on viral evolution. This study describe the discovery, isolation and characterization of Samba virus (SMBV), a novel giant virus belonging to the Mimivirus genus, which was isolated from the Negro River in the Brazilian Amazon. We also report the isolation of an SMBV-associated virophage named Rio Negro (RNV), which is the first Mimivirus virophage to be isolated in the Americas. METHODS/RESULTS: Based on a phylogenetic analysis, SMBV belongs to group A of the putative Megavirales order, possibly a new virus related to Acanthamoeba polyphaga mimivirus (APMV). SMBV is the largest virus isolated in Brazil, with an average particle diameter about 574 nm. The SMBV genome contains 938 ORFs, of which nine are ORFans. The 1,213.6 kb SMBV genome is one of the largest genome of any group A Mimivirus described to date. Electron microscopy showed RNV particle accumulation near SMBV and APMV factories resulting in the production of defective SMBV and APMV particles and decreasing the infectivity of these two viruses by several logs. CONCLUSION: This discovery expands our knowledge of Mimiviridae evolution and ecology. SN - 1743-422X UR - https://www.unboundmedicine.com/medline/citation/24886672/Samba_virus:_a_novel_mimivirus_from_a_giant_rain_forest_the_Brazilian_Amazon_ L2 - https://virologyj.biomedcentral.com/articles/10.1186/1743-422X-11-95 DB - PRIME DP - Unbound Medicine ER -