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Effects of diallyl trisulfide on induction of apoptotic death in murine leukemia WEHI-3 cells in vitro and alterations of the immune responses in normal and leukemic mice in vivo.
Environ Toxicol. 2015 Nov; 30(11):1343-53.ET

Abstract

Diallyl trisulfide (DATS), a chemopreventive dietary constituent and extracted from garlic, has been shown to against cultured many types of human cancer cell liens but the fate of apoptosis in murine leukemia cells in vitro and immune responses in leukemic mice remain elusive. Herein, we clarified the actions of DATS on growth inhibition of murine leukemia WEHI-3 cells in vitro and used WEHI-3 cells to generate leukemic mice in vivo, following to investigate the effects of DATS in animal model. In in vitro study, DATS induced apoptosis of WEHI-3 cells through the G0/G1 phase arrest and induction of caspase-3 activation. In in vivo study DATS decreased the weight of spleen of leukemia mice but did not affect the spleen weight of normal mice. DATS promoted the immune responses such as promotions of the macrophage phagocytosis and NK cell activities in WEHI-3 leukemic and normal mice. However, DATS only promotes NK cell activities in normal mice. DATS increases the surface markers of CD11b and Mac-3 in leukemia mice but only promoted CD3 in normal mice. In conclusion, the present study indicates that DATS induces cell death through induction of apoptosis in mice leukemia WHEI-3 cells. DATS also promotes immune responses in leukemia and normal mice in vivo.

Authors+Show Affiliations

Department of Surgical Intensive Care Unit, Far Eastern Memorial Hospital, New Taipei, 220, Taiwan.Department of Pathology, National Defense Medical Center, Division of Clinical Pathology, Tri-Service General Hospital, Taipei, Taiwan.School of Chinese Medicine, China Medical University, Taichung, 404, Taiwan.Graduate Institute of Chinese Medicine, China Medical University, Taichung, 404, Taiwan. Division of Cardiology, Department of Medicine, China Medical University Hospital, Taichung, 404, Taiwan.School of Post-Baccalaureate Chinese Medicine, China Medical University, Taichung, 404, Taiwan.School of Chinese Medicine, China Medical University, Taichung, 404, Taiwan.Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, St. Martin De Porres Hospital, Chiayi, 600, Taiwan.School of Pharmacy, China Medical University, Taichung, 404, Taiwan.Department of Medical Laboratory Science and Biotechnology, Yuanpei University, Hsinchu, 300, Taiwan.Department of Medical Laboratory Science and Biotechnology, Yuanpei University, Hsinchu, 300, Taiwan.Department of Clinical Pathology, Cheng Hsin General Hospital, Taipei, 112, Taiwan. Department of Restaurant, Hotel and Institutional Management, Fu-Jen Catholic University, New Taipei, 242, Taiwan.Department of Biological Science and Technology, China Medical University, Taichung, 404, Taiwan. Department of Biotechnology, Asia University, Taichung, 413, Taiwan.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24890016

Citation

Hung, Fang-Ming, et al. "Effects of Diallyl Trisulfide On Induction of Apoptotic Death in Murine Leukemia WEHI-3 Cells in Vitro and Alterations of the Immune Responses in Normal and Leukemic Mice in Vivo." Environmental Toxicology, vol. 30, no. 11, 2015, pp. 1343-53.
Hung FM, Shang HS, Tang NY, et al. Effects of diallyl trisulfide on induction of apoptotic death in murine leukemia WEHI-3 cells in vitro and alterations of the immune responses in normal and leukemic mice in vivo. Environ Toxicol. 2015;30(11):1343-53.
Hung, F. M., Shang, H. S., Tang, N. Y., Lin, J. J., Lu, K. W., Lin, J. P., Ko, Y. C., Yu, C. C., Wang, H. L., Liao, J. C., Lu, H. F., & Chung, J. G. (2015). Effects of diallyl trisulfide on induction of apoptotic death in murine leukemia WEHI-3 cells in vitro and alterations of the immune responses in normal and leukemic mice in vivo. Environmental Toxicology, 30(11), 1343-53. https://doi.org/10.1002/tox.22005
Hung FM, et al. Effects of Diallyl Trisulfide On Induction of Apoptotic Death in Murine Leukemia WEHI-3 Cells in Vitro and Alterations of the Immune Responses in Normal and Leukemic Mice in Vivo. Environ Toxicol. 2015;30(11):1343-53. PubMed PMID: 24890016.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of diallyl trisulfide on induction of apoptotic death in murine leukemia WEHI-3 cells in vitro and alterations of the immune responses in normal and leukemic mice in vivo. AU - Hung,Fang-Ming, AU - Shang,Hung-Sheng, AU - Tang,Nou-Ying, AU - Lin,Jen-Jyh, AU - Lu,Kung-Wen, AU - Lin,Jing-Pin, AU - Ko,Yang-Ching, AU - Yu,Chien-Chih, AU - Wang,Hai-Lung, AU - Liao,Jung-Chi, AU - Lu,Hsu-Feng, AU - Chung,Jing-Gung, Y1 - 2014/05/28/ PY - 2014/01/27/received PY - 2014/05/11/accepted PY - 2014/6/4/entrez PY - 2014/6/4/pubmed PY - 2016/5/28/medline KW - DATS KW - WEHI-3 leukemia cells KW - apoptosis KW - caspase-3 KW - immune responses KW - leukemic mice SP - 1343 EP - 53 JF - Environmental toxicology JO - Environ Toxicol VL - 30 IS - 11 N2 - Diallyl trisulfide (DATS), a chemopreventive dietary constituent and extracted from garlic, has been shown to against cultured many types of human cancer cell liens but the fate of apoptosis in murine leukemia cells in vitro and immune responses in leukemic mice remain elusive. Herein, we clarified the actions of DATS on growth inhibition of murine leukemia WEHI-3 cells in vitro and used WEHI-3 cells to generate leukemic mice in vivo, following to investigate the effects of DATS in animal model. In in vitro study, DATS induced apoptosis of WEHI-3 cells through the G0/G1 phase arrest and induction of caspase-3 activation. In in vivo study DATS decreased the weight of spleen of leukemia mice but did not affect the spleen weight of normal mice. DATS promoted the immune responses such as promotions of the macrophage phagocytosis and NK cell activities in WEHI-3 leukemic and normal mice. However, DATS only promotes NK cell activities in normal mice. DATS increases the surface markers of CD11b and Mac-3 in leukemia mice but only promoted CD3 in normal mice. In conclusion, the present study indicates that DATS induces cell death through induction of apoptosis in mice leukemia WHEI-3 cells. DATS also promotes immune responses in leukemia and normal mice in vivo. SN - 1522-7278 UR - https://www.unboundmedicine.com/medline/citation/24890016/Effects_of_diallyl_trisulfide_on_induction_of_apoptotic_death_in_murine_leukemia_WEHI_3_cells_in_vitro_and_alterations_of_the_immune_responses_in_normal_and_leukemic_mice_in_vivo_ L2 - https://doi.org/10.1002/tox.22005 DB - PRIME DP - Unbound Medicine ER -