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Intestinal α-glucosidase and some pancreatic enzymes inhibitory effect of hydroalcholic extract of Moringa stenopetala leaves.

Abstract

BACKGROUND

Moringa stenopetala has been used in traditional health systems to treat diabetes mellitus. One of the successful methods to prevent of the onset of diabetes is to control postprandial hyperglycemia by the inhibition of α-glucosidase and pancreatic α-amylase activities, resulting in the aggressive delay of the carbohydrate digestion of absorbable monosaccharides. The aim of the present study is to investigate the effect of the extract of the leaves of Moringa stenopetala on α-glucosidase, pancreatic α-amylase, pancreatic lipase, and pancreatic cholesterol esterase activities, and, therefore find out the relevance of the plant in controlling blood sugar and lipid levels.

METHODS

The dried leaves of Moringa stenopetala were extracted with hydroalcoholic solvent and dried using rotary vapor under reduced pressure. The dried extracts were determined for the total phenolic compounds, flavonoid content and condensed tannins content by using Folin-Ciocateu's reagent, AlCl3 and vanillin assay, respectively. The dried extract of plant-based food was further quantified with respect to intestinal α-glucosidase (maltase and sucrase) inhibition and pancreatic α-amylase inhibition by glucose oxidase method and dinitrosalicylic (DNS) reagent, respectively.

RESULTS

The phytochemical analysis indicated that flavonoid, total phenolic, and condensed tannin contents in the extract were 71.73 ± 2.48 mg quercetin equivalent/g of crude extract, 79.81 ± 2.85 mg of gallic acid equivalent/g of crude extract, 8.82 ± 0.77 mg catechin equivalent/g of crude extract, respectively. The extract inhibited intestinal sucrase more than intestinal maltase with IC50 value of 1.47 ± 0.19 mg/ml. It also slightly inhibited pancreatic α-amylase, pancreatic lipase and pancreatic cholesterol esterase.

CONCLUSION

The result demonstrated the beneficial biochemical effects of Moringa stenopetala by inhibiting intestinal α-glucosidase, pancreatic cholesterol esterase and pancreatic lipase activities. A daily supplement intake of the leaves of Moringa stenopetala may help in reducing hyperglycemia and hyperlipidemia.

Authors+Show Affiliations

Pharmacology Department, School of Medicine, Addis Ababa University, Addis Ababa, Ethiopia. alemayehutoma@gmail.com.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24890563

Citation

Toma, Alemayehu, et al. "Intestinal Α-glucosidase and some Pancreatic Enzymes Inhibitory Effect of Hydroalcholic Extract of Moringa Stenopetala Leaves." BMC Complementary and Alternative Medicine, vol. 14, 2014, p. 180.
Toma A, Makonnen E, Mekonnen Y, et al. Intestinal α-glucosidase and some pancreatic enzymes inhibitory effect of hydroalcholic extract of Moringa stenopetala leaves. BMC Complement Altern Med. 2014;14:180.
Toma, A., Makonnen, E., Mekonnen, Y., Debella, A., & Addisakwattana, S. (2014). Intestinal α-glucosidase and some pancreatic enzymes inhibitory effect of hydroalcholic extract of Moringa stenopetala leaves. BMC Complementary and Alternative Medicine, 14, p. 180. doi:10.1186/1472-6882-14-180.
Toma A, et al. Intestinal Α-glucosidase and some Pancreatic Enzymes Inhibitory Effect of Hydroalcholic Extract of Moringa Stenopetala Leaves. BMC Complement Altern Med. 2014 Jun 3;14:180. PubMed PMID: 24890563.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Intestinal α-glucosidase and some pancreatic enzymes inhibitory effect of hydroalcholic extract of Moringa stenopetala leaves. AU - Toma,Alemayehu, AU - Makonnen,Eyasu, AU - Mekonnen,Yelamtsehay, AU - Debella,Asfaw, AU - Addisakwattana,Sirichai, Y1 - 2014/06/03/ PY - 2014/01/03/received PY - 2014/05/29/accepted PY - 2014/6/4/entrez PY - 2014/6/4/pubmed PY - 2014/10/21/medline SP - 180 EP - 180 JF - BMC complementary and alternative medicine JO - BMC Complement Altern Med VL - 14 N2 - BACKGROUND: Moringa stenopetala has been used in traditional health systems to treat diabetes mellitus. One of the successful methods to prevent of the onset of diabetes is to control postprandial hyperglycemia by the inhibition of α-glucosidase and pancreatic α-amylase activities, resulting in the aggressive delay of the carbohydrate digestion of absorbable monosaccharides. The aim of the present study is to investigate the effect of the extract of the leaves of Moringa stenopetala on α-glucosidase, pancreatic α-amylase, pancreatic lipase, and pancreatic cholesterol esterase activities, and, therefore find out the relevance of the plant in controlling blood sugar and lipid levels. METHODS: The dried leaves of Moringa stenopetala were extracted with hydroalcoholic solvent and dried using rotary vapor under reduced pressure. The dried extracts were determined for the total phenolic compounds, flavonoid content and condensed tannins content by using Folin-Ciocateu's reagent, AlCl3 and vanillin assay, respectively. The dried extract of plant-based food was further quantified with respect to intestinal α-glucosidase (maltase and sucrase) inhibition and pancreatic α-amylase inhibition by glucose oxidase method and dinitrosalicylic (DNS) reagent, respectively. RESULTS: The phytochemical analysis indicated that flavonoid, total phenolic, and condensed tannin contents in the extract were 71.73 ± 2.48 mg quercetin equivalent/g of crude extract, 79.81 ± 2.85 mg of gallic acid equivalent/g of crude extract, 8.82 ± 0.77 mg catechin equivalent/g of crude extract, respectively. The extract inhibited intestinal sucrase more than intestinal maltase with IC50 value of 1.47 ± 0.19 mg/ml. It also slightly inhibited pancreatic α-amylase, pancreatic lipase and pancreatic cholesterol esterase. CONCLUSION: The result demonstrated the beneficial biochemical effects of Moringa stenopetala by inhibiting intestinal α-glucosidase, pancreatic cholesterol esterase and pancreatic lipase activities. A daily supplement intake of the leaves of Moringa stenopetala may help in reducing hyperglycemia and hyperlipidemia. SN - 1472-6882 UR - https://www.unboundmedicine.com/medline/citation/24890563/Intestinal_α_glucosidase_and_some_pancreatic_enzymes_inhibitory_effect_of_hydroalcholic_extract_of_Moringa_stenopetala_leaves_ L2 - https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/1472-6882-14-180 DB - PRIME DP - Unbound Medicine ER -