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Potential roles of abnormally expressed long noncoding RNA UCA1 and Malat-1 in metastasis of melanoma.
Melanoma Res 2014; 24(4):335-41MR

Abstract

Melanoma is a highly aggressive skin cancer with increasing incidence worldwide. Long noncoding RNAs (lncRNAs), a group of nonprotein-coding transcripts longer than 200 nucleotides, are pervasively transcribed in the genome and are emerging as new players in tumorigenesis. The primary objective of this study was to investigate the role of six cancer-related lncRNAs in pairs of melanoma and adjacent normal tissues (ANTs). A total of 63 primary melanoma, paired ANTs, and metastatic lesions were collected in a Chinese population. Real-time PCR analysis was carried out to compare a series of cancer-related lncRNAs among primary melanoma tissues, ANTs, and metastatic lesions. In in-vitro studies, transwell migration assay was carried out to estimate the migration abilities of melanoma cells with different expression levels of urothelial carcinoma-associated 1 (UCA1) or metastasis-associated lung adenocarcinoma transcript 1 (Malat-1) lncRNAs. We found that UCA1 and Malat-1 lncRNAs were markedly more increased in melanomas than in paired ANTs (P<0.05). Melanomas at later stages (stages 3-4) showed higher expression of UCA1 lncRNA than those at early stages (stages 1-2) (P=0.455). In melanomas with lymph node metastasis, the metastatic lesions had a relatively higher expression of Malat-1 lncRNA than in paired primary tumors (P=0.414). Knockdown of UCA1 or Malat-1 lncRNA could attenuate the migrational ability of melanoma cells in in-vitro studies. Increased expression of UCA1 and Malat-1 lncRNAs might have a correlation with melanoma metastasis.

Authors+Show Affiliations

aDepartment of Dermatology, Beijing Chaoyang Hospital, Capital Medical University, Peking bShenzhen PKU-HKUST Medical Center, Biomedical Research Institute, Guangdong, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24892958

Citation

Tian, Yongjing, et al. "Potential Roles of Abnormally Expressed Long Noncoding RNA UCA1 and Malat-1 in Metastasis of Melanoma." Melanoma Research, vol. 24, no. 4, 2014, pp. 335-41.
Tian Y, Zhang X, Hao Y, et al. Potential roles of abnormally expressed long noncoding RNA UCA1 and Malat-1 in metastasis of melanoma. Melanoma Res. 2014;24(4):335-41.
Tian, Y., Zhang, X., Hao, Y., Fang, Z., & He, Y. (2014). Potential roles of abnormally expressed long noncoding RNA UCA1 and Malat-1 in metastasis of melanoma. Melanoma Research, 24(4), pp. 335-41. doi:10.1097/CMR.0000000000000080.
Tian Y, et al. Potential Roles of Abnormally Expressed Long Noncoding RNA UCA1 and Malat-1 in Metastasis of Melanoma. Melanoma Res. 2014;24(4):335-41. PubMed PMID: 24892958.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Potential roles of abnormally expressed long noncoding RNA UCA1 and Malat-1 in metastasis of melanoma. AU - Tian,Yongjing, AU - Zhang,Xiuying, AU - Hao,Yinghua, AU - Fang,Zhengyu, AU - He,Yanling, PY - 2014/6/4/entrez PY - 2014/6/4/pubmed PY - 2015/4/22/medline SP - 335 EP - 41 JF - Melanoma research JO - Melanoma Res. VL - 24 IS - 4 N2 - Melanoma is a highly aggressive skin cancer with increasing incidence worldwide. Long noncoding RNAs (lncRNAs), a group of nonprotein-coding transcripts longer than 200 nucleotides, are pervasively transcribed in the genome and are emerging as new players in tumorigenesis. The primary objective of this study was to investigate the role of six cancer-related lncRNAs in pairs of melanoma and adjacent normal tissues (ANTs). A total of 63 primary melanoma, paired ANTs, and metastatic lesions were collected in a Chinese population. Real-time PCR analysis was carried out to compare a series of cancer-related lncRNAs among primary melanoma tissues, ANTs, and metastatic lesions. In in-vitro studies, transwell migration assay was carried out to estimate the migration abilities of melanoma cells with different expression levels of urothelial carcinoma-associated 1 (UCA1) or metastasis-associated lung adenocarcinoma transcript 1 (Malat-1) lncRNAs. We found that UCA1 and Malat-1 lncRNAs were markedly more increased in melanomas than in paired ANTs (P<0.05). Melanomas at later stages (stages 3-4) showed higher expression of UCA1 lncRNA than those at early stages (stages 1-2) (P=0.455). In melanomas with lymph node metastasis, the metastatic lesions had a relatively higher expression of Malat-1 lncRNA than in paired primary tumors (P=0.414). Knockdown of UCA1 or Malat-1 lncRNA could attenuate the migrational ability of melanoma cells in in-vitro studies. Increased expression of UCA1 and Malat-1 lncRNAs might have a correlation with melanoma metastasis. SN - 1473-5636 UR - https://www.unboundmedicine.com/medline/citation/24892958/Potential_roles_of_abnormally_expressed_long_noncoding_RNA_UCA1_and_Malat_1_in_metastasis_of_melanoma_ L2 - http://dx.doi.org/10.1097/CMR.0000000000000080 DB - PRIME DP - Unbound Medicine ER -