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Second generation sequencing of three STRs D3S1358, D12S391 and D21S11 in Danes and a new nomenclature for sequenced STR alleles.
Forensic Sci Int Genet. 2014 Sep; 12:38-41.FS

Abstract

Second generation sequencing (SGS) may revolutionize the field of forensic STR typing. Two of the essential requirements for implementation of an SGS based approach for forensic investigations are (1) establishment of adequate frequency databases and (2) adoption of a new STR nomenclature. We report the STR sequences and allele frequencies of three STR loci: D3S1358, D12S391 and D21S11 in 197 unrelated Danes. We used a new STR nomenclature that depicts the locus name used in forensic genetics, the length of the repeat region divided by the repeat length (typically 4 nucleotides) and detailed sequence information of possible sub-repeats and SNPs within the amplified fragment.

Authors+Show Affiliations

Section of Forensic Genetics, Department of Forensic Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Denmark; Faculty of Mathematical, Physical and Natural Sciences, University of Palermo, Palermo, Italy.Section of Forensic Genetics, Department of Forensic Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Denmark. Electronic address: eszter.rockenbauer@sund.ku.dk.Section of Forensic Genetics, Department of Forensic Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Denmark.Section of Forensic Genetics, Department of Forensic Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Denmark.Section of Forensic Genetics, Department of Forensic Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Denmark.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24893347

Citation

Gelardi, Chiara, et al. "Second Generation Sequencing of Three STRs D3S1358, D12S391 and D21S11 in Danes and a New Nomenclature for Sequenced STR Alleles." Forensic Science International. Genetics, vol. 12, 2014, pp. 38-41.
Gelardi C, Rockenbauer E, Dalsgaard S, et al. Second generation sequencing of three STRs D3S1358, D12S391 and D21S11 in Danes and a new nomenclature for sequenced STR alleles. Forensic Sci Int Genet. 2014;12:38-41.
Gelardi, C., Rockenbauer, E., Dalsgaard, S., Børsting, C., & Morling, N. (2014). Second generation sequencing of three STRs D3S1358, D12S391 and D21S11 in Danes and a new nomenclature for sequenced STR alleles. Forensic Science International. Genetics, 12, 38-41. https://doi.org/10.1016/j.fsigen.2014.04.016
Gelardi C, et al. Second Generation Sequencing of Three STRs D3S1358, D12S391 and D21S11 in Danes and a New Nomenclature for Sequenced STR Alleles. Forensic Sci Int Genet. 2014;12:38-41. PubMed PMID: 24893347.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Second generation sequencing of three STRs D3S1358, D12S391 and D21S11 in Danes and a new nomenclature for sequenced STR alleles. AU - Gelardi,Chiara, AU - Rockenbauer,Eszter, AU - Dalsgaard,Sigrun, AU - Børsting,Claus, AU - Morling,Niels, Y1 - 2014/05/10/ PY - 2014/02/24/received PY - 2014/04/29/revised PY - 2014/04/30/accepted PY - 2014/6/4/entrez PY - 2014/6/4/pubmed PY - 2015/4/4/medline KW - Forensic genetics KW - Frequency database KW - Nomenclature KW - STR KW - Second generation sequencing SP - 38 EP - 41 JF - Forensic science international. Genetics JO - Forensic Sci Int Genet VL - 12 N2 - Second generation sequencing (SGS) may revolutionize the field of forensic STR typing. Two of the essential requirements for implementation of an SGS based approach for forensic investigations are (1) establishment of adequate frequency databases and (2) adoption of a new STR nomenclature. We report the STR sequences and allele frequencies of three STR loci: D3S1358, D12S391 and D21S11 in 197 unrelated Danes. We used a new STR nomenclature that depicts the locus name used in forensic genetics, the length of the repeat region divided by the repeat length (typically 4 nucleotides) and detailed sequence information of possible sub-repeats and SNPs within the amplified fragment. SN - 1878-0326 UR - https://www.unboundmedicine.com/medline/citation/24893347/Second_generation_sequencing_of_three_STRs_D3S1358_D12S391_and_D21S11_in_Danes_and_a_new_nomenclature_for_sequenced_STR_alleles_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1872-4973(14)00093-3 DB - PRIME DP - Unbound Medicine ER -