Tags

Type your tag names separated by a space and hit enter

Simplified procedure of silymarin extraction from Silybum marianum L. Gaertner.
J Chromatogr Sci. 2015 Feb; 53(2):366-72.JC

Abstract

Silymarin, a mixture of flavonolignans exhibiting many pharmacological activities, is obtained from the fruits of milk thistle (Silybum marianum L. Gaertner). Due to the high lipid content in thistle fruits, the European Pharmacopoeia recommends a two-step process of its extraction. First, the fruits are defatted for 6 h, using n-hexane; second, silymarin is extracted with methanol for 5 more hours. The presented data show that this extremely long traditional Soxhlet extraction process can be shortened to a few minutes using pressurized liquid extraction (PLE). PLE also allows to eliminate the defatting stage required in the traditional procedure, thus simplifying the silymarin extraction procedure and preventing silymarin loss caused by defatting. The PLE recoveries obtained under the optimized extraction conditions are clearly better than the ones obtained by the Pharmacopoeia-recommended Soxhlet extraction procedure. The PLE yields of silychristin, silydianin, silybin A, silybin B, isosilybin A and isosilybin B in acetone are 3.3, 6.9, 3.3, 5.1, 2.6 and 1.5 mg/g of the non-defatted fruits, respectively. The 5-h Soxhlet extraction with methanol on defatted fruits gives only ∼72% of the silymarin amount obtained in 10 min PLE at 125°C.

Authors+Show Affiliations

Faculty of Chemistry, Department of Chromatographic Methods, Maria Curie-Sklodowska University, Pl. Maria Curie-Sklodowska 3, Lublin 20-031, Poland dorota.wianowska@poczta.umcs.lublin.pl.Faculty of Chemistry, Department of Chromatographic Methods, Maria Curie-Sklodowska University, Pl. Maria Curie-Sklodowska 3, Lublin 20-031, Poland.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

24895445

Citation

Wianowska, Dorota, and Mariusz Wiśniewski. "Simplified Procedure of Silymarin Extraction From Silybum Marianum L. Gaertner." Journal of Chromatographic Science, vol. 53, no. 2, 2015, pp. 366-72.
Wianowska D, Wiśniewski M. Simplified procedure of silymarin extraction from Silybum marianum L. Gaertner. J Chromatogr Sci. 2015;53(2):366-72.
Wianowska, D., & Wiśniewski, M. (2015). Simplified procedure of silymarin extraction from Silybum marianum L. Gaertner. Journal of Chromatographic Science, 53(2), 366-72. https://doi.org/10.1093/chromsci/bmu049
Wianowska D, Wiśniewski M. Simplified Procedure of Silymarin Extraction From Silybum Marianum L. Gaertner. J Chromatogr Sci. 2015;53(2):366-72. PubMed PMID: 24895445.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simplified procedure of silymarin extraction from Silybum marianum L. Gaertner. AU - Wianowska,Dorota, AU - Wiśniewski,Mariusz, Y1 - 2014/06/03/ PY - 2014/6/5/entrez PY - 2014/6/5/pubmed PY - 2015/9/29/medline SP - 366 EP - 72 JF - Journal of chromatographic science JO - J Chromatogr Sci VL - 53 IS - 2 N2 - Silymarin, a mixture of flavonolignans exhibiting many pharmacological activities, is obtained from the fruits of milk thistle (Silybum marianum L. Gaertner). Due to the high lipid content in thistle fruits, the European Pharmacopoeia recommends a two-step process of its extraction. First, the fruits are defatted for 6 h, using n-hexane; second, silymarin is extracted with methanol for 5 more hours. The presented data show that this extremely long traditional Soxhlet extraction process can be shortened to a few minutes using pressurized liquid extraction (PLE). PLE also allows to eliminate the defatting stage required in the traditional procedure, thus simplifying the silymarin extraction procedure and preventing silymarin loss caused by defatting. The PLE recoveries obtained under the optimized extraction conditions are clearly better than the ones obtained by the Pharmacopoeia-recommended Soxhlet extraction procedure. The PLE yields of silychristin, silydianin, silybin A, silybin B, isosilybin A and isosilybin B in acetone are 3.3, 6.9, 3.3, 5.1, 2.6 and 1.5 mg/g of the non-defatted fruits, respectively. The 5-h Soxhlet extraction with methanol on defatted fruits gives only ∼72% of the silymarin amount obtained in 10 min PLE at 125°C. SN - 1945-239X UR - https://www.unboundmedicine.com/medline/citation/24895445/Simplified_procedure_of_silymarin_extraction_from_Silybum_marianum_L__Gaertner_ L2 - https://academic.oup.com/chromsci/article-lookup/doi/10.1093/chromsci/bmu049 DB - PRIME DP - Unbound Medicine ER -