Tags

Type your tag names separated by a space and hit enter

Anti-inflammatory effects of Perilla frutescens leaf extract on lipopolysaccharide-stimulated RAW264.7 cells.
Mol Med Rep 2014; 10(2):1077-83MM

Abstract

Perilla leaves are widely used in Chinese herbal medicine and in Japanese herbal agents used to treat respiratory diseases. This study aimed to investigate the anti‑inflammatory effects and the underlying mechanisms of Perilla frutescens leaf extract (PLE). Murine macrophage RAW264.7 cells were used as a model. Cell viability and morphological changes were studied by the MTT assay and microscopy. mRNA expression of pro‑inflammatory mediators was assessed by both semi‑quantitative reverse transcription‑polymerase chain reaction (RT‑PCR) and quantitative (q) RT‑PCR. Nitric oxide (NO) and prostaglandin E2 (PGE2) production were analyzed by the Griess test and sandwich enzyme‑linked immunosorbent assay (ELISA), respectively. The activation of kinase cascades was studied by immunoblotting. Our findings showed that PLE slightly affects cell viability, but alleviates LPS‑induced activation of RAW264.7 cells. Furthermore, PLE significantly reduced the LPS‑induced mRNA expression of the interleukin (IL)‑6, IL‑8, tumor necrosis factor‑α (TNF‑α), cyclooxygenase‑2 (COX‑2) and inducible nitric oxide synthase (iNOS), genes in a dose‑dependent manner. In addition, PLE reduced NO production and PGE2 secretion induced by LPS. PLE also inhibited activation of mitogen‑activated protein kinases (MAPKs), increased the cytosolic IκBα level, and reduced the level of nuclear factor (NF)‑κB. Taken together, these findings indicate that PLE significantly decreases the mRNA expression and protein production of pro‑inflammatory mediators, via the inhibition of extracellular‑signal‑regulated kinase (ERK)1/2, c‑Jun N‑terminal kinase (JNK), p38, as well as NF‑κB signaling in RAW264.7 cells stimulated with LPS.

Authors+Show Affiliations

Department of Pathology, Tungs' Taichung MetroHarbor Hospital, Taichung, Taiwan, R.O.C.Institute of Biochemistry and Biotechnology, Chung Shan Medical University Hospital, Taichung, Taiwan, R.O.C.Institute of Biochemistry and Biotechnology, Chung Shan Medical University Hospital, Taichung, Taiwan, R.O.C.Institute of Biochemistry and Biotechnology, Chung Shan Medical University Hospital, Taichung, Taiwan, R.O.C.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24898576

Citation

Huang, Bee-Piao, et al. "Anti-inflammatory Effects of Perilla Frutescens Leaf Extract On Lipopolysaccharide-stimulated RAW264.7 Cells." Molecular Medicine Reports, vol. 10, no. 2, 2014, pp. 1077-83.
Huang BP, Lin CH, Chen YC, et al. Anti-inflammatory effects of Perilla frutescens leaf extract on lipopolysaccharide-stimulated RAW264.7 cells. Mol Med Rep. 2014;10(2):1077-83.
Huang, B. P., Lin, C. H., Chen, Y. C., & Kao, S. H. (2014). Anti-inflammatory effects of Perilla frutescens leaf extract on lipopolysaccharide-stimulated RAW264.7 cells. Molecular Medicine Reports, 10(2), pp. 1077-83. doi:10.3892/mmr.2014.2298.
Huang BP, et al. Anti-inflammatory Effects of Perilla Frutescens Leaf Extract On Lipopolysaccharide-stimulated RAW264.7 Cells. Mol Med Rep. 2014;10(2):1077-83. PubMed PMID: 24898576.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Anti-inflammatory effects of Perilla frutescens leaf extract on lipopolysaccharide-stimulated RAW264.7 cells. AU - Huang,Bee-Piao, AU - Lin,Chun-Hsiang, AU - Chen,Yi-Ching, AU - Kao,Shao-Hsuan, Y1 - 2014/06/05/ PY - 2013/10/25/received PY - 2014/03/28/accepted PY - 2014/6/6/entrez PY - 2014/6/6/pubmed PY - 2015/2/13/medline SP - 1077 EP - 83 JF - Molecular medicine reports JO - Mol Med Rep VL - 10 IS - 2 N2 - Perilla leaves are widely used in Chinese herbal medicine and in Japanese herbal agents used to treat respiratory diseases. This study aimed to investigate the anti‑inflammatory effects and the underlying mechanisms of Perilla frutescens leaf extract (PLE). Murine macrophage RAW264.7 cells were used as a model. Cell viability and morphological changes were studied by the MTT assay and microscopy. mRNA expression of pro‑inflammatory mediators was assessed by both semi‑quantitative reverse transcription‑polymerase chain reaction (RT‑PCR) and quantitative (q) RT‑PCR. Nitric oxide (NO) and prostaglandin E2 (PGE2) production were analyzed by the Griess test and sandwich enzyme‑linked immunosorbent assay (ELISA), respectively. The activation of kinase cascades was studied by immunoblotting. Our findings showed that PLE slightly affects cell viability, but alleviates LPS‑induced activation of RAW264.7 cells. Furthermore, PLE significantly reduced the LPS‑induced mRNA expression of the interleukin (IL)‑6, IL‑8, tumor necrosis factor‑α (TNF‑α), cyclooxygenase‑2 (COX‑2) and inducible nitric oxide synthase (iNOS), genes in a dose‑dependent manner. In addition, PLE reduced NO production and PGE2 secretion induced by LPS. PLE also inhibited activation of mitogen‑activated protein kinases (MAPKs), increased the cytosolic IκBα level, and reduced the level of nuclear factor (NF)‑κB. Taken together, these findings indicate that PLE significantly decreases the mRNA expression and protein production of pro‑inflammatory mediators, via the inhibition of extracellular‑signal‑regulated kinase (ERK)1/2, c‑Jun N‑terminal kinase (JNK), p38, as well as NF‑κB signaling in RAW264.7 cells stimulated with LPS. SN - 1791-3004 UR - https://www.unboundmedicine.com/medline/citation/24898576/Anti_inflammatory_effects_of_Perilla_frutescens_leaf_extract_on_lipopolysaccharide_stimulated_RAW264_7_cells_ L2 - http://www.spandidos-publications.com/mmr/10/2/1077 DB - PRIME DP - Unbound Medicine ER -