Tags

Type your tag names separated by a space and hit enter

Identification, cloning and characterization of the tomato TCP transcription factor family.
BMC Plant Biol. 2014 Jun 06; 14:157.BP

Abstract

BACKGROUND

TCP proteins are plant-specific transcription factors, which are known to have a wide range of functions in different plant species such as in leaf development, flower symmetry, shoot branching, and senescence. Only a small number of TCP genes has been characterised from tomato (Solanum lycopersicum). Here we report several functional features of the members of the entire family present in the tomato genome.

RESULTS

We have identified 30 Solanum lycopersicum SlTCP genes, most of which have not been described before. Phylogenetic analysis clearly distinguishes two homology classes of the SlTCP transcription factor family - class I and class II. Class II differentiates in two subclasses, the CIN-TCP subclass and the CYC/TB1 subclass, involved in leaf development and axillary shoots formation, respectively. The expression patterns of all members were determined by quantitative PCR. Several SlTCP genes, like SlTCP12, SlTCP15 and SlTCP18 are preferentially expressed in the tomato fruit, suggesting a role during fruit development or ripening. These genes are regulated by RIN (RIPENING INHIBITOR), CNR (COLORLESS NON-RIPENING) and SlAP2a (APETALA2a) proteins, which are transcription factors with key roles in ripening. With a yeast one-hybrid assay we demonstrated that RIN binds the promoter fragments of SlTCP12, SlTCP15 and SlTCP18, and that CNR binds the SlTCP18 promoter. This data strongly suggests that these class I SlTCP proteins are involved in ripening. Furthermore, we demonstrate that SlTCPs bind the promoter fragments of members of their own family, indicating that they regulate each other. Additional yeast one-hybrid studies performed with Arabidopsis transcription factors revealed binding of the promoter fragments by proteins involved in the ethylene signal transduction pathway, contributing to the idea that these SlTCP genes are involved in the ripening process. Yeast two-hybrid data shows that SlTCP proteins can form homo and heterodimers, suggesting that they act together in order to form functional protein complexes and together regulate developmental processes in tomato.

CONCLUSIONS

The comprehensive analysis we performed, like phylogenetic analysis, expression studies, identification of the upstream regulators and the dimerization specificity of the tomato TCP transcription factor family provides the basis for functional studies to reveal the role of this family in tomato development.

Authors+Show Affiliations

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availablePlant Research International, P,O, Box 619, 6700 AP Wageningen, the Netherlands. ruud.demaagd@wur.nl.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24903607

Citation

Parapunova, Violeta, et al. "Identification, Cloning and Characterization of the Tomato TCP Transcription Factor Family." BMC Plant Biology, vol. 14, 2014, p. 157.
Parapunova V, Busscher M, Busscher-Lange J, et al. Identification, cloning and characterization of the tomato TCP transcription factor family. BMC Plant Biol. 2014;14:157.
Parapunova, V., Busscher, M., Busscher-Lange, J., Lammers, M., Karlova, R., Bovy, A. G., Angenent, G. C., & de Maagd, R. A. (2014). Identification, cloning and characterization of the tomato TCP transcription factor family. BMC Plant Biology, 14, 157. https://doi.org/10.1186/1471-2229-14-157
Parapunova V, et al. Identification, Cloning and Characterization of the Tomato TCP Transcription Factor Family. BMC Plant Biol. 2014 Jun 6;14:157. PubMed PMID: 24903607.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification, cloning and characterization of the tomato TCP transcription factor family. AU - Parapunova,Violeta, AU - Busscher,Marco, AU - Busscher-Lange,Jacqueline, AU - Lammers,Michiel, AU - Karlova,Rumyana, AU - Bovy,Arnaud G, AU - Angenent,Gerco C, AU - de Maagd,Ruud A, Y1 - 2014/06/06/ PY - 2014/02/21/received PY - 2014/05/22/accepted PY - 2014/6/7/entrez PY - 2014/6/7/pubmed PY - 2015/1/17/medline SP - 157 EP - 157 JF - BMC plant biology JO - BMC Plant Biol VL - 14 N2 - BACKGROUND: TCP proteins are plant-specific transcription factors, which are known to have a wide range of functions in different plant species such as in leaf development, flower symmetry, shoot branching, and senescence. Only a small number of TCP genes has been characterised from tomato (Solanum lycopersicum). Here we report several functional features of the members of the entire family present in the tomato genome. RESULTS: We have identified 30 Solanum lycopersicum SlTCP genes, most of which have not been described before. Phylogenetic analysis clearly distinguishes two homology classes of the SlTCP transcription factor family - class I and class II. Class II differentiates in two subclasses, the CIN-TCP subclass and the CYC/TB1 subclass, involved in leaf development and axillary shoots formation, respectively. The expression patterns of all members were determined by quantitative PCR. Several SlTCP genes, like SlTCP12, SlTCP15 and SlTCP18 are preferentially expressed in the tomato fruit, suggesting a role during fruit development or ripening. These genes are regulated by RIN (RIPENING INHIBITOR), CNR (COLORLESS NON-RIPENING) and SlAP2a (APETALA2a) proteins, which are transcription factors with key roles in ripening. With a yeast one-hybrid assay we demonstrated that RIN binds the promoter fragments of SlTCP12, SlTCP15 and SlTCP18, and that CNR binds the SlTCP18 promoter. This data strongly suggests that these class I SlTCP proteins are involved in ripening. Furthermore, we demonstrate that SlTCPs bind the promoter fragments of members of their own family, indicating that they regulate each other. Additional yeast one-hybrid studies performed with Arabidopsis transcription factors revealed binding of the promoter fragments by proteins involved in the ethylene signal transduction pathway, contributing to the idea that these SlTCP genes are involved in the ripening process. Yeast two-hybrid data shows that SlTCP proteins can form homo and heterodimers, suggesting that they act together in order to form functional protein complexes and together regulate developmental processes in tomato. CONCLUSIONS: The comprehensive analysis we performed, like phylogenetic analysis, expression studies, identification of the upstream regulators and the dimerization specificity of the tomato TCP transcription factor family provides the basis for functional studies to reveal the role of this family in tomato development. SN - 1471-2229 UR - https://www.unboundmedicine.com/medline/citation/24903607/Identification_cloning_and_characterization_of_the_tomato_TCP_transcription_factor_family_ L2 - https://bmcplantbiol.biomedcentral.com/articles/10.1186/1471-2229-14-157 DB - PRIME DP - Unbound Medicine ER -