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Effects of natural honey on polymicrobial culture of various human pathogens.
Arch Med Sci 2014; 10(2):246-50AM

Abstract

INTRODUCTION

Honey has a wide range of antimicrobial activity. All previous studies have considered honey's effect on a single microbe. The present study investigated activity of honey towards a high dose of single or polymicrobial culture.

MATERIAL AND METHODS

10 µl specimens of Staphylococcus aureus (S. aureus), Streptococcus pyogenes (S. pyogenes), Escherichia coli (E. coli) and Candida albicans (C. albicans) were cultured in 10 ml of 10-100% (wt/v) honey diluted in broth. Six types of polymicrobial microbial cultures were prepared by culturing the isolates with each other onto broth (control) and broth containing various concentrations of honey (10-100% wt/v). Microbial growth was assessed on solid plate media after 24 h incubation.

RESULTS

Honey (30-70%) prevents growth of 10 µl specimens of all the isolates. Greater reduction in growth of E. coli was observed when cultured with S. aureus. Culturing of S. aureus with S. pyogenes, C. albicans, or E. coli increased its sensitivity to honey. S. aureus and S. pyogenes increased sensitivity of C. albicans to honey while E. coli and C. albicans decreased sensitivity of S. pyogenes.

CONCLUSIONS

It might be concluded that honey prevents and inhibits growth of single and polymicrobial pathogenic cultures. Polymicrobial culture affects growth of the isolates and increases their sensitivity to honey.

Authors+Show Affiliations

Al-Waili's Foundation for Science, New York, USA.Dubai Specialized Medical Center, Islamic Establishment for Education, Dubai, United Arab Emirates.Dubai Specialized Medical Center, Islamic Establishment for Education, Dubai, United Arab Emirates.Dubai Specialized Medical Center, Islamic Establishment for Education, Dubai, United Arab Emirates.Al-Waili's Foundation for Science, New York, USA.Bee Research Chair, King Saud University, Riyadh, Kingdom of Saudi Arabi.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

24904656

Citation

Al-Waili, Noori S., et al. "Effects of Natural Honey On Polymicrobial Culture of Various Human Pathogens." Archives of Medical Science : AMS, vol. 10, no. 2, 2014, pp. 246-50.
Al-Waili NS, Al-Waili FS, Akmal M, et al. Effects of natural honey on polymicrobial culture of various human pathogens. Arch Med Sci. 2014;10(2):246-50.
Al-Waili, N. S., Al-Waili, F. S., Akmal, M., Ali, A., Salom, K. Y., & Al Ghamdi, A. A. (2014). Effects of natural honey on polymicrobial culture of various human pathogens. Archives of Medical Science : AMS, 10(2), pp. 246-50. doi:10.5114/aoms.2012.28603.
Al-Waili NS, et al. Effects of Natural Honey On Polymicrobial Culture of Various Human Pathogens. Arch Med Sci. 2014 May 12;10(2):246-50. PubMed PMID: 24904656.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of natural honey on polymicrobial culture of various human pathogens. AU - Al-Waili,Noori S, AU - Al-Waili,Faiza S, AU - Akmal,Mohammed, AU - Ali,Amjed, AU - Salom,Khelod Y, AU - Al Ghamdi,Ahmad A, Y1 - 2012/05/15/ PY - 2011/01/01/received PY - 2011/05/19/revised PY - 2011/06/09/accepted PY - 2014/6/7/entrez PY - 2014/6/7/pubmed PY - 2014/6/7/medline KW - Candida albicans KW - Escherichia coli KW - Staphylococcus aureus KW - Streptococcus pyogenes KW - honey SP - 246 EP - 50 JF - Archives of medical science : AMS JO - Arch Med Sci VL - 10 IS - 2 N2 - INTRODUCTION: Honey has a wide range of antimicrobial activity. All previous studies have considered honey's effect on a single microbe. The present study investigated activity of honey towards a high dose of single or polymicrobial culture. MATERIAL AND METHODS: 10 µl specimens of Staphylococcus aureus (S. aureus), Streptococcus pyogenes (S. pyogenes), Escherichia coli (E. coli) and Candida albicans (C. albicans) were cultured in 10 ml of 10-100% (wt/v) honey diluted in broth. Six types of polymicrobial microbial cultures were prepared by culturing the isolates with each other onto broth (control) and broth containing various concentrations of honey (10-100% wt/v). Microbial growth was assessed on solid plate media after 24 h incubation. RESULTS: Honey (30-70%) prevents growth of 10 µl specimens of all the isolates. Greater reduction in growth of E. coli was observed when cultured with S. aureus. Culturing of S. aureus with S. pyogenes, C. albicans, or E. coli increased its sensitivity to honey. S. aureus and S. pyogenes increased sensitivity of C. albicans to honey while E. coli and C. albicans decreased sensitivity of S. pyogenes. CONCLUSIONS: It might be concluded that honey prevents and inhibits growth of single and polymicrobial pathogenic cultures. Polymicrobial culture affects growth of the isolates and increases their sensitivity to honey. SN - 1734-1922 UR - https://www.unboundmedicine.com/medline/citation/24904656/Effects_of_natural_honey_on_polymicrobial_culture_of_various_human_pathogens_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24904656/ DB - PRIME DP - Unbound Medicine ER -