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Developmental validation of the PowerPlex(®) Fusion System for analysis of casework and reference samples: A 24-locus multiplex for new database standards.
Forensic Sci Int Genet. 2014 Sep; 12:69-76.FS

Abstract

The original CODIS database based on 13 core STR loci has been overwhelmingly successful for matching suspects with evidence. Yet there remain situations that argue for inclusion of more loci and increased discrimination. The PowerPlex(®) Fusion System allows simultaneous amplification of the following loci: Amelogenin, D3S1358, D1S1656, D2S441, D10S1248, D13S317, Penta E, D16S539, D18S51, D2S1338, CSF1PO, Penta D, TH01, vWA, D21S11, D7S820, D5S818, TPOX, DYS391, D8S1179, D12S391, D19S433, FGA, and D22S1045. The comprehensive list of loci amplified by the system generates a profile compatible with databases based on either the expanded CODIS or European Standard Set (ESS) requirements. Developmental validation testing followed SWGDAM guidelines and demonstrated the quality and robustness of the PowerPlex(®) Fusion System across a number of variables. Consistent and high-quality results were compiled using data from 12 separate forensic and research laboratories. The results verify that the PowerPlex(®) Fusion System is a robust and reliable STR-typing multiplex suitable for human identification.

Authors+Show Affiliations

Promega Corporation, 2800 Woods Hollow Road, Madison, WI 53711, USA. Electronic address: katie.oostdik@promega.com.Promega Corporation, 2800 Woods Hollow Road, Madison, WI 53711, USA. Electronic address: kristy.lenz@promega.com.Michigan State Police, 333 South Grand Avenue, P.O. Box 30634, Lansing, MI 48909, USA. Electronic address: NyeJ1@michigan.gov.Michigan State Police, 333 South Grand Avenue, P.O. Box 30634, Lansing, MI 48909, USA. Electronic address: schellingk@michigan.gov.Michigan State Police, 333 South Grand Avenue, P.O. Box 30634, Lansing, MI 48909, USA. Electronic address: yetd@michigan.gov.Michigan State Police, 333 South Grand Avenue, P.O. Box 30634, Lansing, MI 48909, USA. Electronic address: bruskis@michigan.gov.Michigan State Police, 333 South Grand Avenue, P.O. Box 30634, Lansing, MI 48909, USA. Electronic address: strongj@michigan.gov.United States Army Criminal Investigation Laboratory, 4930 North 31st Street, Forest Park, GA 30297, USA(1). Electronic address: clint.d.buchanan.civ@mail.mil.United States Army Criminal Investigation Laboratory, 4930 North 31st Street, Forest Park, GA 30297, USA(1). Electronic address: joel.d.sutton2.civ@mail.mil.United States Army Criminal Investigation Laboratory, 4930 North 31st Street, Forest Park, GA 30297, USA(1). Electronic address: jessica.k.linner.civ@mail.mil.United States Army Criminal Investigation Laboratory, 4930 North 31st Street, Forest Park, GA 30297, USA(1). Electronic address: nicole.r.frazier.civ@mail.mil.Arkansas State Crime Laboratory, 3 Natural Resources Drive, Little Rock, AR 72205, USA. Electronic address: hays.young@crimelab.arkansas.gov.Los Angeles County Sheriff's Department, 1800 Paseo Rancho Castilla, Los Angeles, CA 90032, USA. Electronic address: LKMatthi@lasd.org.Los Angeles County Sheriff's Department, 1800 Paseo Rancho Castilla, Los Angeles, CA 90032, USA. Electronic address: ARSage@lasd.org.Kansas Bureau of Investigation, Forensic Laboratory Division, 1620 Southwest Tyler Street, Topeka, KS 66612, USA. Electronic address: jeff.hahn@kbi.state.ks.us.Kentucky State Police, 100 Sower Boulevard, Frankfort, KY 40601, USA. Electronic address: regina.wells@ky.gov.Washington State Patrol, Crime Laboratory Division, 2203 Airport Way South, Seattle, WA 98134-2028, USA. Electronic address: Natasha.williams@wsp.wa.gov.Washington State Patrol, Crime Laboratory Division, 2203 Airport Way South, Seattle, WA 98134-2028, USA. Electronic address: monica.price@wsp.wa.gov.Texas Department of Public Safety, Crime Laboratory, 5805 North Lamar Boulevard, Austin, TX 78752, USA. Electronic address: jody.koehler@dps.texas.gov.New Hampshire State Police, Forensic Laboratory, 33 Hazen Drive, Concord, NH 03305, USA. Electronic address: melisa.staples@dos.nh.gov.New Hampshire State Police, Forensic Laboratory, 33 Hazen Drive, Concord, NH 03305, USA. Electronic address: katie.swango@dos.nh.gov.National Institute of Standards and Technology, Biochemical Science Division, 100 Bureau Drive, Gaithersburg, MA 20899, USA. Electronic address: becky.hill@nist.gov.Oklahoma State Bureau of Investigation, 800 East 2nd Street, Edmond, OK 73034, USA. Electronic address: karen.oyerly@osbi.ok.gov.Oklahoma State Bureau of Investigation, 800 East 2nd Street, Edmond, OK 73034, USA. Electronic address: wendy.duke@osbi.ok.gov.Oklahoma State Bureau of Investigation, 800 East 2nd Street, Edmond, OK 73034, USA. Electronic address: Lesley.katzilierakis@osbi.ok.gov.Promega Corporation, 2800 Woods Hollow Road, Madison, WI 53711, USA. Electronic address: martin.ensenberger@promega.com.Promega Corporation, 2800 Woods Hollow Road, Madison, WI 53711, USA. Electronic address: jeanne.bourdeau@promega.com.Promega Corporation, 2800 Woods Hollow Road, Madison, WI 53711, USA. Electronic address: benjamin.krenke@promega.com.Promega Corporation, 2800 Woods Hollow Road, Madison, WI 53711, USA. Electronic address: cindy.sprecher@promega.com.Promega Corporation, 2800 Woods Hollow Road, Madison, WI 53711, USA. Electronic address: doug.storts@promega.com.

Pub Type(s)

Journal Article
Validation Study

Language

eng

PubMed ID

24905335

Citation

Oostdik, Kathryn, et al. "Developmental Validation of the PowerPlex(®) Fusion System for Analysis of Casework and Reference Samples: a 24-locus Multiplex for New Database Standards." Forensic Science International. Genetics, vol. 12, 2014, pp. 69-76.
Oostdik K, Lenz K, Nye J, et al. Developmental validation of the PowerPlex(®) Fusion System for analysis of casework and reference samples: A 24-locus multiplex for new database standards. Forensic Sci Int Genet. 2014;12:69-76.
Oostdik, K., Lenz, K., Nye, J., Schelling, K., Yet, D., Bruski, S., Strong, J., Buchanan, C., Sutton, J., Linner, J., Frazier, N., Young, H., Matthies, L., Sage, A., Hahn, J., Wells, R., Williams, N., Price, M., Koehler, J., ... Storts, D. R. (2014). Developmental validation of the PowerPlex(®) Fusion System for analysis of casework and reference samples: A 24-locus multiplex for new database standards. Forensic Science International. Genetics, 12, 69-76. https://doi.org/10.1016/j.fsigen.2014.04.013
Oostdik K, et al. Developmental Validation of the PowerPlex(®) Fusion System for Analysis of Casework and Reference Samples: a 24-locus Multiplex for New Database Standards. Forensic Sci Int Genet. 2014;12:69-76. PubMed PMID: 24905335.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Developmental validation of the PowerPlex(®) Fusion System for analysis of casework and reference samples: A 24-locus multiplex for new database standards. AU - Oostdik,Kathryn, AU - Lenz,Kristy, AU - Nye,Jeffrey, AU - Schelling,Kristin, AU - Yet,Donald, AU - Bruski,Scott, AU - Strong,Joshua, AU - Buchanan,Clint, AU - Sutton,Joel, AU - Linner,Jessica, AU - Frazier,Nicole, AU - Young,Hays, AU - Matthies,Learden, AU - Sage,Amber, AU - Hahn,Jeff, AU - Wells,Regina, AU - Williams,Natasha, AU - Price,Monica, AU - Koehler,Jody, AU - Staples,Melisa, AU - Swango,Katie L, AU - Hill,Carolyn, AU - Oyerly,Karen, AU - Duke,Wendy, AU - Katzilierakis,Lesley, AU - Ensenberger,Martin G, AU - Bourdeau,Jeanne M, AU - Sprecher,Cynthia J, AU - Krenke,Benjamin, AU - Storts,Douglas R, Y1 - 2014/05/14/ PY - 2013/03/25/received PY - 2014/04/04/revised PY - 2014/04/28/accepted PY - 2014/6/7/entrez PY - 2014/6/7/pubmed PY - 2015/4/4/medline KW - Forensic science KW - PowerPlex(R) KW - Short tandem repeat KW - Validation SP - 69 EP - 76 JF - Forensic science international. Genetics JO - Forensic Sci Int Genet VL - 12 N2 - The original CODIS database based on 13 core STR loci has been overwhelmingly successful for matching suspects with evidence. Yet there remain situations that argue for inclusion of more loci and increased discrimination. The PowerPlex(®) Fusion System allows simultaneous amplification of the following loci: Amelogenin, D3S1358, D1S1656, D2S441, D10S1248, D13S317, Penta E, D16S539, D18S51, D2S1338, CSF1PO, Penta D, TH01, vWA, D21S11, D7S820, D5S818, TPOX, DYS391, D8S1179, D12S391, D19S433, FGA, and D22S1045. The comprehensive list of loci amplified by the system generates a profile compatible with databases based on either the expanded CODIS or European Standard Set (ESS) requirements. Developmental validation testing followed SWGDAM guidelines and demonstrated the quality and robustness of the PowerPlex(®) Fusion System across a number of variables. Consistent and high-quality results were compiled using data from 12 separate forensic and research laboratories. The results verify that the PowerPlex(®) Fusion System is a robust and reliable STR-typing multiplex suitable for human identification. SN - 1878-0326 UR - https://www.unboundmedicine.com/medline/citation/24905335/Developmental_validation_of_the_PowerPlex_®__Fusion_System_for_analysis_of_casework_and_reference_samples:_A_24_locus_multiplex_for_new_database_standards_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1872-4973(14)00089-1 DB - PRIME DP - Unbound Medicine ER -