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HPLC method for the simultaneous determination of pilocarpine, isopilocarpine, pilocarpic acid and isopilocarpic acid.
J Pharm Biomed Anal 1989; 7(12):1729-34JP

Abstract

A high-performance liquid chromatographic method suitable for the determination of pilocarpine, isopilocarpine, pilocarpic acid and isopilocarpic acid is described. The column used is a Spherisorb-CN bonded phase at 25 degrees C. An aqueous solution of triethylamine (0.1%, v/v) at pH 2.5 is used as mobile phase. Peak detection is by UV absorption at 220 nm. The elution orders are found to be pilocarpic-isopilocarpic acids with a resolution of 9 and K' of 5 and 6, respectively, isopilocarpine-pilocarpine with a resolution of 1.8 and K' of 13 and 13.5, respectively, the peaks being symmetrical.

Authors+Show Affiliations

Departamento de Investigación, Laboratorios Cusí, Barcelona, Spain.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

2490561

Citation

Gomez-Gomar, A, et al. "HPLC Method for the Simultaneous Determination of Pilocarpine, Isopilocarpine, Pilocarpic Acid and Isopilocarpic Acid." Journal of Pharmaceutical and Biomedical Analysis, vol. 7, no. 12, 1989, pp. 1729-34.
Gomez-Gomar A, Gonzalez-Aubert M, Costa-Segarra J. HPLC method for the simultaneous determination of pilocarpine, isopilocarpine, pilocarpic acid and isopilocarpic acid. J Pharm Biomed Anal. 1989;7(12):1729-34.
Gomez-Gomar, A., Gonzalez-Aubert, M., & Costa-Segarra, J. (1989). HPLC method for the simultaneous determination of pilocarpine, isopilocarpine, pilocarpic acid and isopilocarpic acid. Journal of Pharmaceutical and Biomedical Analysis, 7(12), pp. 1729-34.
Gomez-Gomar A, Gonzalez-Aubert M, Costa-Segarra J. HPLC Method for the Simultaneous Determination of Pilocarpine, Isopilocarpine, Pilocarpic Acid and Isopilocarpic Acid. J Pharm Biomed Anal. 1989;7(12):1729-34. PubMed PMID: 2490561.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - HPLC method for the simultaneous determination of pilocarpine, isopilocarpine, pilocarpic acid and isopilocarpic acid. AU - Gomez-Gomar,A, AU - Gonzalez-Aubert,M, AU - Costa-Segarra,J, PY - 1989/1/1/pubmed PY - 1989/1/1/medline PY - 1989/1/1/entrez SP - 1729 EP - 34 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 7 IS - 12 N2 - A high-performance liquid chromatographic method suitable for the determination of pilocarpine, isopilocarpine, pilocarpic acid and isopilocarpic acid is described. The column used is a Spherisorb-CN bonded phase at 25 degrees C. An aqueous solution of triethylamine (0.1%, v/v) at pH 2.5 is used as mobile phase. Peak detection is by UV absorption at 220 nm. The elution orders are found to be pilocarpic-isopilocarpic acids with a resolution of 9 and K' of 5 and 6, respectively, isopilocarpine-pilocarpine with a resolution of 1.8 and K' of 13 and 13.5, respectively, the peaks being symmetrical. SN - 0731-7085 UR - https://www.unboundmedicine.com/medline/citation/2490561/HPLC_method_for_the_simultaneous_determination_of_pilocarpine_isopilocarpine_pilocarpic_acid_and_isopilocarpic_acid_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0731-7085(89)80187-6 DB - PRIME DP - Unbound Medicine ER -