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A three-line lateral flow assay strip for the measurement of C-reactive protein covering a broad physiological concentration range in human sera.
Biosens Bioelectron. 2014 Nov 15; 61:285-9.BB

Abstract

The lateral flow assay (LFA) strip sensor possesses many advantages as a diagnostic device, including the capabilities of rapid, one-step assay performance, and high throughput production. A major limitation of the sensor, however, is its difficulty in measuring a broad concentration range of target proteins, including C-reactive protein (CRP), due to the "hook effect." In this study, we report the use of a three-line LFA strip sensor, adding an antigen line to the conventional two-line LFA sensor, for detecting CRP within a broad concentration range in human sera. We introduced an antigen line between test and control lines in the LFA sensor. The antigen line was formed by dispensing a CRP antibody solution followed by a CRP solution in nitrocellulose membrane. All other conditions were identical to those applied to the conventional LFA strip sensor. The CRP level in test samples was generated by data processing from the intensities of three lines. The strip sensor measured a linear detection range of CRP concentration from 1 ng/mL to 500 μg/mL within 10 min, with a calculated detection range of 0.69 ng/mL-1.02 mg/mL. Using the developed three-line LFA sensor, 50 clinical samples were measured at a detection range of 0.4-84.7 μg/mL. This novel and easy-to-use CRP sensor can be a useful tool for rapid, sensitive, and cost-effective detection of a broad physiological concentration range of CRP capabilities that are vital for various diagnostic applications.

Authors+Show Affiliations

INGIbio Co. Ltd., R&D Center, 206, APRI, 123 Chemdan-gwagiro, Buk-gu, Gwangju 500-712, Republic of Korea.School of Physics and Chemistry, Gwangju Institute of Science and Technology, Gwangju 500-712, Republic of Korea.Department of Physiology, Kyungpook National University School of Medicine, Daegu 700-422, Republic of Korea.Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA 02139, United States.INGIbio Co. Ltd., R&D Center, 206, APRI, 123 Chemdan-gwagiro, Buk-gu, Gwangju 500-712, Republic of Korea; School of Physics and Chemistry, Gwangju Institute of Science and Technology, Gwangju 500-712, Republic of Korea. Electronic address: mkim@gist.ac.kr.

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24906087

Citation

Oh, Young Kyoung, et al. "A Three-line Lateral Flow Assay Strip for the Measurement of C-reactive Protein Covering a Broad Physiological Concentration Range in Human Sera." Biosensors & Bioelectronics, vol. 61, 2014, pp. 285-9.
Oh YK, Joung HA, Han HS, et al. A three-line lateral flow assay strip for the measurement of C-reactive protein covering a broad physiological concentration range in human sera. Biosens Bioelectron. 2014;61:285-9.
Oh, Y. K., Joung, H. A., Han, H. S., Suk, H. J., & Kim, M. G. (2014). A three-line lateral flow assay strip for the measurement of C-reactive protein covering a broad physiological concentration range in human sera. Biosensors & Bioelectronics, 61, 285-9. https://doi.org/10.1016/j.bios.2014.04.032
Oh YK, et al. A Three-line Lateral Flow Assay Strip for the Measurement of C-reactive Protein Covering a Broad Physiological Concentration Range in Human Sera. Biosens Bioelectron. 2014 Nov 15;61:285-9. PubMed PMID: 24906087.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A three-line lateral flow assay strip for the measurement of C-reactive protein covering a broad physiological concentration range in human sera. AU - Oh,Young Kyoung, AU - Joung,Hyou-Arm, AU - Han,Hyung Soo, AU - Suk,Ho-Jun, AU - Kim,Min-Gon, Y1 - 2014/05/14/ PY - 2014/01/13/received PY - 2014/04/01/revised PY - 2014/04/17/accepted PY - 2014/6/7/entrez PY - 2014/6/7/pubmed PY - 2015/2/24/medline KW - C-reactive protein KW - Gold nanoparticle KW - Hook effect KW - Lateral flow assay KW - Sandwich immunoassay SP - 285 EP - 9 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 61 N2 - The lateral flow assay (LFA) strip sensor possesses many advantages as a diagnostic device, including the capabilities of rapid, one-step assay performance, and high throughput production. A major limitation of the sensor, however, is its difficulty in measuring a broad concentration range of target proteins, including C-reactive protein (CRP), due to the "hook effect." In this study, we report the use of a three-line LFA strip sensor, adding an antigen line to the conventional two-line LFA sensor, for detecting CRP within a broad concentration range in human sera. We introduced an antigen line between test and control lines in the LFA sensor. The antigen line was formed by dispensing a CRP antibody solution followed by a CRP solution in nitrocellulose membrane. All other conditions were identical to those applied to the conventional LFA strip sensor. The CRP level in test samples was generated by data processing from the intensities of three lines. The strip sensor measured a linear detection range of CRP concentration from 1 ng/mL to 500 μg/mL within 10 min, with a calculated detection range of 0.69 ng/mL-1.02 mg/mL. Using the developed three-line LFA sensor, 50 clinical samples were measured at a detection range of 0.4-84.7 μg/mL. This novel and easy-to-use CRP sensor can be a useful tool for rapid, sensitive, and cost-effective detection of a broad physiological concentration range of CRP capabilities that are vital for various diagnostic applications. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/24906087/A_three_line_lateral_flow_assay_strip_for_the_measurement_of_C_reactive_protein_covering_a_broad_physiological_concentration_range_in_human_sera_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(14)00299-1 DB - PRIME DP - Unbound Medicine ER -