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Downregulation of miR-21 increases cisplatin sensitivity of non-small-cell lung cancer.
Cancer Genet. 2014 May; 207(5):214-20.CG

Abstract

Recent studies have shown that plasma miR-21 is a biomarker of chemotherapeutic response in lung cancer, but the influence of miR-21 on the sensitivity of non-small-cell lung cancer (NSCLC) to cisplatin (DDP) has not been confirmed. The aim of this study was to evaluate the role of miR-21 in NSCLC sensitivity to DDP in vitro and in vivo. Real-time quantitative PCR was used to detect miR-21 expression in lung cancer cell lines. Synthesized locked nucleic acid (LNA) anti-miR-21 was transiently transfected into A549 cells and pre-miR-21 was transfected into SK-MES-1 cells. We also investigated the effects of miR-21 downregulation and upregulation on growth and colony formation in DDP-treated cells. Finally, the effect of miR-21 downregulation on in vivo sensitivity of A549 cells to DDP was determined in BALB/c nude mice. miR-21 expression was significantly higher in A549 than in other lung cancer cell lines. LNA-based knockdown of miR-21 significantly inhibited growth and induced death in A549 cells, possibly via apoptotic signaling. Pre-miR-21 significantly promoted growth and inhibited death in SK-MES-1 cells. Moreover, ectopic suppression of miR-21 sensitized A549 cells to DDP in vivo. Our findings demonstrate that miR-21 suppression enhances the sensitivity of lung cancer cells to DDP in vitro and in vivo.

Authors+Show Affiliations

Cell and Molecular Biology Laboratory, Zhoushan Hospital, Zhejiang, China.Cell and Molecular Biology Laboratory, Zhoushan Hospital, Zhejiang, China.Cell and Molecular Biology Laboratory, Zhoushan Hospital, Zhejiang, China.Cell and Molecular Biology Laboratory, Zhoushan Hospital, Zhejiang, China.Cell and Molecular Biology Laboratory, Zhoushan Hospital, Zhejiang, China.Department of Cardio-Thoracic surgery, Zhoushan Hospital, Zhejiang, China. Electronic address: zyk801801@126.com.Cell and Molecular Biology Laboratory, Zhoushan Hospital, Zhejiang, China. Electronic address: lxg2004@gmail.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24906642

Citation

Xu, Liyun, et al. "Downregulation of miR-21 Increases Cisplatin Sensitivity of Non-small-cell Lung Cancer." Cancer Genetics, vol. 207, no. 5, 2014, pp. 214-20.
Xu L, Huang Y, Chen D, et al. Downregulation of miR-21 increases cisplatin sensitivity of non-small-cell lung cancer. Cancer Genet. 2014;207(5):214-20.
Xu, L., Huang, Y., Chen, D., He, J., Zhu, W., Zhang, Y., & Liu, X. (2014). Downregulation of miR-21 increases cisplatin sensitivity of non-small-cell lung cancer. Cancer Genetics, 207(5), 214-20. https://doi.org/10.1016/j.cancergen.2014.04.003
Xu L, et al. Downregulation of miR-21 Increases Cisplatin Sensitivity of Non-small-cell Lung Cancer. Cancer Genet. 2014;207(5):214-20. PubMed PMID: 24906642.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Downregulation of miR-21 increases cisplatin sensitivity of non-small-cell lung cancer. AU - Xu,Liyun, AU - Huang,Yanyan, AU - Chen,Dongdong, AU - He,Jianying, AU - Zhu,Wangyu, AU - Zhang,Yongkui, AU - Liu,Xiaoguang, Y1 - 2014/04/13/ PY - 2013/10/31/received PY - 2014/03/17/revised PY - 2014/04/02/accepted PY - 2014/6/8/entrez PY - 2014/6/8/pubmed PY - 2014/10/8/medline KW - A549 KW - LNA anti–miR-21 KW - chemotherapy KW - cisplatin KW - lung cancer SP - 214 EP - 20 JF - Cancer genetics JO - Cancer Genet VL - 207 IS - 5 N2 - Recent studies have shown that plasma miR-21 is a biomarker of chemotherapeutic response in lung cancer, but the influence of miR-21 on the sensitivity of non-small-cell lung cancer (NSCLC) to cisplatin (DDP) has not been confirmed. The aim of this study was to evaluate the role of miR-21 in NSCLC sensitivity to DDP in vitro and in vivo. Real-time quantitative PCR was used to detect miR-21 expression in lung cancer cell lines. Synthesized locked nucleic acid (LNA) anti-miR-21 was transiently transfected into A549 cells and pre-miR-21 was transfected into SK-MES-1 cells. We also investigated the effects of miR-21 downregulation and upregulation on growth and colony formation in DDP-treated cells. Finally, the effect of miR-21 downregulation on in vivo sensitivity of A549 cells to DDP was determined in BALB/c nude mice. miR-21 expression was significantly higher in A549 than in other lung cancer cell lines. LNA-based knockdown of miR-21 significantly inhibited growth and induced death in A549 cells, possibly via apoptotic signaling. Pre-miR-21 significantly promoted growth and inhibited death in SK-MES-1 cells. Moreover, ectopic suppression of miR-21 sensitized A549 cells to DDP in vivo. Our findings demonstrate that miR-21 suppression enhances the sensitivity of lung cancer cells to DDP in vitro and in vivo. SN - 2210-7762 UR - https://www.unboundmedicine.com/medline/citation/24906642/Downregulation_of_miR_21_increases_cisplatin_sensitivity_of_non_small_cell_lung_cancer_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S2210-7762(14)00070-2 DB - PRIME DP - Unbound Medicine ER -