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High performance liquid chromatography coupled to electrospray ionization and quadrupole time-of-flight-mass spectrometry as a powerful analytical strategy for systematic analysis and improved characterization of the major bioactive constituents from Radix Dipsaci.
J Pharm Biomed Anal. 2014 Sep; 98:120-9.JP

Abstract

Radix Dipsaci (RD), the dried root of Dipsacus asper, is commonly used as a traditional Chinese medicine for the treatment of bone diseases and functions in strengthening bone and healing bone fractures. Nevertheless, the high polarity, non chromophores and low abundance of multiple compounds in this plant bring difficulty for their isolation and structural determination by traditional chromatographic and spectroscopic techniques, which hindered the use of RD in clinical practice and retarded the process of RD modernization. In this work, a sensitive and rapid high-performance liquid chromatography coupled with electrospray time-of-flight-mass spectrometry (HPLC-ESI-QTOF-MS/MS) was employed to rapidly separate and identify the multiple minor constituents in RD. Separation was performed an Agilent poroshell 120 EC-C18 column (2.1mm×100mm, i.d., 2.7μm) with 0.1% formic acid aqueous solution and acetonitrile as the mobile phase under gradient conditions. As a result, 36 major constituents including dipsacus saponins, iridoid glycosides and caffeoyl quinic acid derivatives were identified or tentatively characterized from the RD, 11 of which had not been previously reported to the best of our knowledge. In conclusion, the HPLC-ESI-QTOF-MS/MS is feasible and credible technique to separate and identify the constituents in complex matrices of traditional Chinese medicines.

Authors+Show Affiliations

College of Life Sciences, Huzhou Teachers College, Huzhou 313000, People's Republic of China; College of Life Sciences, Jiujiang University, Jiujiang 332005, People's Republic of China. Electronic address: lymanuxt@126.com.College of Life Sciences, Jiujiang University, Jiujiang 332005, People's Republic of China.College of Life Sciences, Jiujiang University, Jiujiang 332005, People's Republic of China.College of Life Sciences, Jiujiang University, Jiujiang 332005, People's Republic of China.College of Life Sciences, Huzhou Teachers College, Huzhou 313000, People's Republic of China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24907650

Citation

Ling, Yun, et al. "High Performance Liquid Chromatography Coupled to Electrospray Ionization and Quadrupole Time-of-flight-mass Spectrometry as a Powerful Analytical Strategy for Systematic Analysis and Improved Characterization of the Major Bioactive Constituents From Radix Dipsaci." Journal of Pharmaceutical and Biomedical Analysis, vol. 98, 2014, pp. 120-9.
Ling Y, Liu K, Zhang Q, et al. High performance liquid chromatography coupled to electrospray ionization and quadrupole time-of-flight-mass spectrometry as a powerful analytical strategy for systematic analysis and improved characterization of the major bioactive constituents from Radix Dipsaci. J Pharm Biomed Anal. 2014;98:120-9.
Ling, Y., Liu, K., Zhang, Q., Liao, L., & Lu, Y. (2014). High performance liquid chromatography coupled to electrospray ionization and quadrupole time-of-flight-mass spectrometry as a powerful analytical strategy for systematic analysis and improved characterization of the major bioactive constituents from Radix Dipsaci. Journal of Pharmaceutical and Biomedical Analysis, 98, 120-9. https://doi.org/10.1016/j.jpba.2014.05.006
Ling Y, et al. High Performance Liquid Chromatography Coupled to Electrospray Ionization and Quadrupole Time-of-flight-mass Spectrometry as a Powerful Analytical Strategy for Systematic Analysis and Improved Characterization of the Major Bioactive Constituents From Radix Dipsaci. J Pharm Biomed Anal. 2014;98:120-9. PubMed PMID: 24907650.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - High performance liquid chromatography coupled to electrospray ionization and quadrupole time-of-flight-mass spectrometry as a powerful analytical strategy for systematic analysis and improved characterization of the major bioactive constituents from Radix Dipsaci. AU - Ling,Yun, AU - Liu,Keyue, AU - Zhang,Qing, AU - Liao,Liang, AU - Lu,Yunhua, Y1 - 2014/05/16/ PY - 2013/12/27/received PY - 2014/05/07/revised PY - 2014/05/09/accepted PY - 2014/6/8/entrez PY - 2014/6/8/pubmed PY - 2015/4/22/medline KW - Caffeoyl quinic acid KW - Constituents identification KW - Dipsacus saponins KW - HPLC-ESI-QTOF–MS/MS KW - Iridoid glycosides KW - Radix Dipsaci SP - 120 EP - 9 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 98 N2 - Radix Dipsaci (RD), the dried root of Dipsacus asper, is commonly used as a traditional Chinese medicine for the treatment of bone diseases and functions in strengthening bone and healing bone fractures. Nevertheless, the high polarity, non chromophores and low abundance of multiple compounds in this plant bring difficulty for their isolation and structural determination by traditional chromatographic and spectroscopic techniques, which hindered the use of RD in clinical practice and retarded the process of RD modernization. In this work, a sensitive and rapid high-performance liquid chromatography coupled with electrospray time-of-flight-mass spectrometry (HPLC-ESI-QTOF-MS/MS) was employed to rapidly separate and identify the multiple minor constituents in RD. Separation was performed an Agilent poroshell 120 EC-C18 column (2.1mm×100mm, i.d., 2.7μm) with 0.1% formic acid aqueous solution and acetonitrile as the mobile phase under gradient conditions. As a result, 36 major constituents including dipsacus saponins, iridoid glycosides and caffeoyl quinic acid derivatives were identified or tentatively characterized from the RD, 11 of which had not been previously reported to the best of our knowledge. In conclusion, the HPLC-ESI-QTOF-MS/MS is feasible and credible technique to separate and identify the constituents in complex matrices of traditional Chinese medicines. SN - 1873-264X UR - https://www.unboundmedicine.com/medline/citation/24907650/High_performance_liquid_chromatography_coupled_to_electrospray_ionization_and_quadrupole_time_of_flight_mass_spectrometry_as_a_powerful_analytical_strategy_for_systematic_analysis_and_improved_characterization_of_the_major_bioactive_constituents_from_Radix_Dipsaci_ DB - PRIME DP - Unbound Medicine ER -