Tags

Type your tag names separated by a space and hit enter

Zinc oxide influences mitogen-activated protein kinase and TGF-β1 signaling pathways, and enhances intestinal barrier integrity in weaned pigs.
Innate Immun. 2015 May; 21(4):341-8.II

Abstract

Weaning is the most significant event in the life of pigs and is always related with intestinal disruption. Although it is well known that zinc oxide (ZnO) exerts beneficial effects on the intestinal barrier, the mechanisms underlying these effects have not yet been fully elucidated. We examined whether ZnO protects the intestinal barrier via mitogen-activated protein kinases and TGF-β1 signaling pathways. Twelve barrows weaned at 21 d of age were randomly assigned to two treatments (0 verus 2200 mg Zn/kg from ZnO) for 1 wk. The results showed that supplementation with ZnO increased daily gain and feed intake, and decreased postweaning scour scores. ZnO improved intestinal morphology, as indicated by increased villus height and villus height:crypt depth ratio, and intestinal barrier function, indicated by increased transepithelial electrical resistance and decreased mucosal-to-serosal permeability to 4-ku FITC dextran. ZnO decreased the ratios of the phosphorylated to total JNK and p38 (p-JNK/JNK and p-p38/p38), while it increased the ratio of ERK (p-ERK/ERK). Supplementation with ZnO increased intestinal TGF-β1 expression. The results indicate that supplementation with ZnO activates ERK ½, and inhibits JNK and p38 signaling pathways, and increases intestinal TGF-β1 expression in weaned pigs.

Authors+Show Affiliations

Animal Science College, Zhejiang University, Hangzhou, PR China The Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Hangzhou, PR China.Animal Science College, Zhejiang University, Hangzhou, PR China The Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Hangzhou, PR China.Animal Science College, Zhejiang University, Hangzhou, PR China The Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Hangzhou, PR China.Animal Science College, Zhejiang University, Hangzhou, PR China The Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Hangzhou, PR China.Animal Science College, Zhejiang University, Hangzhou, PR China The Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Hangzhou, PR China chhu@zju.edu.cn.

Pub Type(s)

Clinical Trial
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24917655

Citation

Song, Ze He, et al. "Zinc Oxide Influences Mitogen-activated Protein Kinase and TGF-β1 Signaling Pathways, and Enhances Intestinal Barrier Integrity in Weaned Pigs." Innate Immunity, vol. 21, no. 4, 2015, pp. 341-8.
Song ZH, Xiao K, Ke YL, et al. Zinc oxide influences mitogen-activated protein kinase and TGF-β1 signaling pathways, and enhances intestinal barrier integrity in weaned pigs. Innate Immun. 2015;21(4):341-8.
Song, Z. H., Xiao, K., Ke, Y. L., Jiao, l. e. . F., & Hu, C. H. (2015). Zinc oxide influences mitogen-activated protein kinase and TGF-β1 signaling pathways, and enhances intestinal barrier integrity in weaned pigs. Innate Immunity, 21(4), 341-8. https://doi.org/10.1177/1753425914536450
Song ZH, et al. Zinc Oxide Influences Mitogen-activated Protein Kinase and TGF-β1 Signaling Pathways, and Enhances Intestinal Barrier Integrity in Weaned Pigs. Innate Immun. 2015;21(4):341-8. PubMed PMID: 24917655.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Zinc oxide influences mitogen-activated protein kinase and TGF-β1 signaling pathways, and enhances intestinal barrier integrity in weaned pigs. AU - Song,Ze He, AU - Xiao,Kan, AU - Ke,Ya Lu, AU - Jiao,Le Fei, AU - Hu,Cai Hong, Y1 - 2014/06/10/ PY - 2014/01/26/received PY - 2014/04/29/accepted PY - 2014/6/12/entrez PY - 2014/6/12/pubmed PY - 2016/1/16/medline KW - Zinc oxide KW - intestinal barrier KW - mitogen-activated protein kinases KW - transforming growth factor-β1 KW - weanling pigs SP - 341 EP - 8 JF - Innate immunity JO - Innate Immun VL - 21 IS - 4 N2 - Weaning is the most significant event in the life of pigs and is always related with intestinal disruption. Although it is well known that zinc oxide (ZnO) exerts beneficial effects on the intestinal barrier, the mechanisms underlying these effects have not yet been fully elucidated. We examined whether ZnO protects the intestinal barrier via mitogen-activated protein kinases and TGF-β1 signaling pathways. Twelve barrows weaned at 21 d of age were randomly assigned to two treatments (0 verus 2200 mg Zn/kg from ZnO) for 1 wk. The results showed that supplementation with ZnO increased daily gain and feed intake, and decreased postweaning scour scores. ZnO improved intestinal morphology, as indicated by increased villus height and villus height:crypt depth ratio, and intestinal barrier function, indicated by increased transepithelial electrical resistance and decreased mucosal-to-serosal permeability to 4-ku FITC dextran. ZnO decreased the ratios of the phosphorylated to total JNK and p38 (p-JNK/JNK and p-p38/p38), while it increased the ratio of ERK (p-ERK/ERK). Supplementation with ZnO increased intestinal TGF-β1 expression. The results indicate that supplementation with ZnO activates ERK ½, and inhibits JNK and p38 signaling pathways, and increases intestinal TGF-β1 expression in weaned pigs. SN - 1753-4267 UR - https://www.unboundmedicine.com/medline/citation/24917655/Zinc_oxide_influences_mitogen_activated_protein_kinase_and_TGF_β1_signaling_pathways_and_enhances_intestinal_barrier_integrity_in_weaned_pigs_ L2 - https://journals.sagepub.com/doi/10.1177/1753425914536450?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -