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Storage of human biospecimens: selection of the optimal storage temperature.
Biopreserv Biobank 2014; 12(3):165-75BB

Abstract

Millions of biological samples are currently kept at low tempertures in cryobanks/biorepositories for long-term storage. The quality of the biospecimen when thawed, however, is not only determined by processing of the biospecimen but the storage conditions as well. The overall objective of this article is to describe the scientific basis for selecting a storage temperature for a biospecimen based on current scientific understanding. To that end, this article reviews some physical basics of the temperature, nucleation, and ice crystal growth present in biological samples stored at low temperatures (-20°C to -196°C), and our current understanding of the role of temperature on the activity of degradative molecules present in biospecimens. The scientific literature relevant to the stability of specific biomarkers in human fluid, cell, and tissue biospecimens is also summarized for the range of temperatures between -20°C to -196°C. These studies demonstrate the importance of storage temperature on the stability of critical biomarkers for fluid, cell, and tissue biospecimens.

Authors+Show Affiliations

1 Biopreservation Core Resource, University of Minnesota , Minneapolis, Minnesota.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Review

Language

eng

PubMed ID

24918763

Citation

Hubel, Allison, et al. "Storage of Human Biospecimens: Selection of the Optimal Storage Temperature." Biopreservation and Biobanking, vol. 12, no. 3, 2014, pp. 165-75.
Hubel A, Spindler R, Skubitz AP. Storage of human biospecimens: selection of the optimal storage temperature. Biopreserv Biobank. 2014;12(3):165-75.
Hubel, A., Spindler, R., & Skubitz, A. P. (2014). Storage of human biospecimens: selection of the optimal storage temperature. Biopreservation and Biobanking, 12(3), pp. 165-75. doi:10.1089/bio.2013.0084.
Hubel A, Spindler R, Skubitz AP. Storage of Human Biospecimens: Selection of the Optimal Storage Temperature. Biopreserv Biobank. 2014;12(3):165-75. PubMed PMID: 24918763.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Storage of human biospecimens: selection of the optimal storage temperature. AU - Hubel,Allison, AU - Spindler,Ralf, AU - Skubitz,Amy P N, Y1 - 2014/06/11/ PY - 2014/6/12/entrez PY - 2014/6/12/pubmed PY - 2015/1/27/medline SP - 165 EP - 75 JF - Biopreservation and biobanking JO - Biopreserv Biobank VL - 12 IS - 3 N2 - Millions of biological samples are currently kept at low tempertures in cryobanks/biorepositories for long-term storage. The quality of the biospecimen when thawed, however, is not only determined by processing of the biospecimen but the storage conditions as well. The overall objective of this article is to describe the scientific basis for selecting a storage temperature for a biospecimen based on current scientific understanding. To that end, this article reviews some physical basics of the temperature, nucleation, and ice crystal growth present in biological samples stored at low temperatures (-20°C to -196°C), and our current understanding of the role of temperature on the activity of degradative molecules present in biospecimens. The scientific literature relevant to the stability of specific biomarkers in human fluid, cell, and tissue biospecimens is also summarized for the range of temperatures between -20°C to -196°C. These studies demonstrate the importance of storage temperature on the stability of critical biomarkers for fluid, cell, and tissue biospecimens. SN - 1947-5543 UR - https://www.unboundmedicine.com/medline/citation/24918763/Storage_of_human_biospecimens:_selection_of_the_optimal_storage_temperature_ L2 - https://www.liebertpub.com/doi/full/10.1089/bio.2013.0084?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -