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Regulation of monocyte/macrophage C2 production and HLA-DR expression by IL-4 (BSF-1) and IFN-gamma.
J Immunol. 1989 Jan 15; 142(2):520-5.JI

Abstract

IL-4 was originally described on the basis of its ability to co-stimulate the proliferation of resting B cells treated with anti-IgM. Recently, this cytokine has been shown to have other effects on mast cells, T cells, B cells, and macrophages. We studied the ability of IL-4 to regulate the production of C2 by human monocytes and monocytic cell lines and compared this with stimulation of HLA-DR expression, another recently described activity of IL-4. Responses to IL-4 were compared to IFN-gamma, a cytokine with both activities. IL-4 up-regulated C2 production by human monocytes and this effect was not inhibited by neutralizing anti-IFN-gamma antibody. IL-4 also stimulated C2 production by HL-60 cells that had been pre-treated with vitamin D3 to induce monocytic differentiation. IL-4 did not stimulate C2 production by U937 cells. IFN-gamma, in contrast to IL-4, stimulates C2 production by all three cell types. Although IL-4 increased C2 production by HL-60 cells we could not detect C2 mRNA by Northern blotting. However, co-stimulation of these cells with IL-4 and low concentrations of IFN-gamma resulted in an additive effect on C2 production and a greater increase in C2 mRNA than was seen with IFN-gamma alone. As reported by others, IL-4-stimulated HLA-DR expression by monocytes. In contrast to our findings regarding C2 production, stimulation of HLA-DR expression was inhibited by neutralizing anti-IFN-gamma mAb and IL-4 did not stimulate HLA-DR expression by U937 or HL-60 cells. IFN-gamma stimulated HLA-DR expression by all three cell types. These results identify IL-4 as an additional cytokine able to directly stimulate C2 production by human monocytes and by a monocytic cell line whereas IL-4 stimulation of HLA-DR expression by monocytes appears to be IFN-gamma dependent.

Authors+Show Affiliations

Rheumatology Section, McGuire V.A. Medical Center, Richmond, VA 23249.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

2492049

Citation

Littman, B H., et al. "Regulation of Monocyte/macrophage C2 Production and HLA-DR Expression By IL-4 (BSF-1) and IFN-gamma." Journal of Immunology (Baltimore, Md. : 1950), vol. 142, no. 2, 1989, pp. 520-5.
Littman BH, Dastvan FF, Carlson PL, et al. Regulation of monocyte/macrophage C2 production and HLA-DR expression by IL-4 (BSF-1) and IFN-gamma. J Immunol. 1989;142(2):520-5.
Littman, B. H., Dastvan, F. F., Carlson, P. L., & Sanders, K. M. (1989). Regulation of monocyte/macrophage C2 production and HLA-DR expression by IL-4 (BSF-1) and IFN-gamma. Journal of Immunology (Baltimore, Md. : 1950), 142(2), 520-5.
Littman BH, et al. Regulation of Monocyte/macrophage C2 Production and HLA-DR Expression By IL-4 (BSF-1) and IFN-gamma. J Immunol. 1989 Jan 15;142(2):520-5. PubMed PMID: 2492049.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Regulation of monocyte/macrophage C2 production and HLA-DR expression by IL-4 (BSF-1) and IFN-gamma. AU - Littman,B H, AU - Dastvan,F F, AU - Carlson,P L, AU - Sanders,K M, PY - 1989/1/15/pubmed PY - 1989/1/15/medline PY - 1989/1/15/entrez SP - 520 EP - 5 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 142 IS - 2 N2 - IL-4 was originally described on the basis of its ability to co-stimulate the proliferation of resting B cells treated with anti-IgM. Recently, this cytokine has been shown to have other effects on mast cells, T cells, B cells, and macrophages. We studied the ability of IL-4 to regulate the production of C2 by human monocytes and monocytic cell lines and compared this with stimulation of HLA-DR expression, another recently described activity of IL-4. Responses to IL-4 were compared to IFN-gamma, a cytokine with both activities. IL-4 up-regulated C2 production by human monocytes and this effect was not inhibited by neutralizing anti-IFN-gamma antibody. IL-4 also stimulated C2 production by HL-60 cells that had been pre-treated with vitamin D3 to induce monocytic differentiation. IL-4 did not stimulate C2 production by U937 cells. IFN-gamma, in contrast to IL-4, stimulates C2 production by all three cell types. Although IL-4 increased C2 production by HL-60 cells we could not detect C2 mRNA by Northern blotting. However, co-stimulation of these cells with IL-4 and low concentrations of IFN-gamma resulted in an additive effect on C2 production and a greater increase in C2 mRNA than was seen with IFN-gamma alone. As reported by others, IL-4-stimulated HLA-DR expression by monocytes. In contrast to our findings regarding C2 production, stimulation of HLA-DR expression was inhibited by neutralizing anti-IFN-gamma mAb and IL-4 did not stimulate HLA-DR expression by U937 or HL-60 cells. IFN-gamma stimulated HLA-DR expression by all three cell types. These results identify IL-4 as an additional cytokine able to directly stimulate C2 production by human monocytes and by a monocytic cell line whereas IL-4 stimulation of HLA-DR expression by monocytes appears to be IFN-gamma dependent. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/2492049/Regulation_of_monocyte/macrophage_C2_production_and_HLA_DR_expression_by_IL_4__BSF_1__and_IFN_gamma_ L2 - https://www.jimmunol.org/lookup/pmidlookup?view=long&pmid=2492049 DB - PRIME DP - Unbound Medicine ER -