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Protective effect of bioflavonoid myricetin enhances carbohydrate metabolic enzymes and insulin signaling molecules in streptozotocin-cadmium induced diabetic nephrotoxic rats.
Toxicol Appl Pharmacol. 2014 Sep 01; 279(2):173-85.TA

Abstract

Diabetic nephropathy is the kidney disease that occurs as a result of diabetes. The present study was aimed to evaluate the therapeutic potential of myricetin by assaying the activities of key enzymes of carbohydrate metabolism, insulin signaling molecules and renal function markers in streptozotocin (STZ)-cadmium (Cd) induced diabetic nephrotoxic rats. After myricetin treatment schedule, blood and tissue samples were collected to determine plasma glucose, insulin, hemoglobin, glycosylated hemoglobin and renal function markers, carbohydrate metabolic enzymes in the liver and insulin signaling molecules in the pancreas and skeletal muscle. A significant increase of plasma glucose, glycosylated hemoglobin, urea, uric acid, creatinine, blood urea nitrogen (BUN), urinary albumin, glycogen phosphorylase, glucose-6-phosphatase, and fructose-1,6-bisphosphatase and a significant decrease of plasma insulin, hemoglobin, hexokinase, glucose-6-phosphate dehydrogenase, glycogen and glycogen synthase with insulin signaling molecule expression were found in the STZ-Cd induced diabetic nephrotoxic rats. The administration of myricetin significantly normalizes the carbohydrate metabolic products like glucose, glycated hemoglobin, glycogen phosphorylase and gluconeogenic enzymes and renal function markers with increase insulin, glycogen, glycogen synthase and insulin signaling molecule expression like glucose transporter-2 (GLUT-2), glucose transporter-4 (GLUT-4), insulin receptor-1 (IRS-1), insulin receptor-2 (IRS-2) and protein kinase B (PKB). Based on the data, the protective effect of myricetin was confirmed by its histological annotation of the pancreas, liver and kidney tissues. These findings suggest that myricetin improved carbohydrate metabolism which subsequently enhances glucose utilization and renal function in STZ-Cd induced diabetic nephrotoxic rats.

Authors+Show Affiliations

Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalai Nagar 608 002, Tamil Nadu, India.Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalai Nagar 608 002, Tamil Nadu, India. Electronic address: npashokkumar1@gmail.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24923654

Citation

Kandasamy, Neelamegam, and Natarajan Ashokkumar. "Protective Effect of Bioflavonoid Myricetin Enhances Carbohydrate Metabolic Enzymes and Insulin Signaling Molecules in Streptozotocin-cadmium Induced Diabetic Nephrotoxic Rats." Toxicology and Applied Pharmacology, vol. 279, no. 2, 2014, pp. 173-85.
Kandasamy N, Ashokkumar N. Protective effect of bioflavonoid myricetin enhances carbohydrate metabolic enzymes and insulin signaling molecules in streptozotocin-cadmium induced diabetic nephrotoxic rats. Toxicol Appl Pharmacol. 2014;279(2):173-85.
Kandasamy, N., & Ashokkumar, N. (2014). Protective effect of bioflavonoid myricetin enhances carbohydrate metabolic enzymes and insulin signaling molecules in streptozotocin-cadmium induced diabetic nephrotoxic rats. Toxicology and Applied Pharmacology, 279(2), 173-85. https://doi.org/10.1016/j.taap.2014.05.014
Kandasamy N, Ashokkumar N. Protective Effect of Bioflavonoid Myricetin Enhances Carbohydrate Metabolic Enzymes and Insulin Signaling Molecules in Streptozotocin-cadmium Induced Diabetic Nephrotoxic Rats. Toxicol Appl Pharmacol. 2014 Sep 1;279(2):173-85. PubMed PMID: 24923654.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective effect of bioflavonoid myricetin enhances carbohydrate metabolic enzymes and insulin signaling molecules in streptozotocin-cadmium induced diabetic nephrotoxic rats. AU - Kandasamy,Neelamegam, AU - Ashokkumar,Natarajan, Y1 - 2014/06/09/ PY - 2013/08/23/received PY - 2014/05/11/revised PY - 2014/05/28/accepted PY - 2014/6/14/entrez PY - 2014/6/14/pubmed PY - 2014/10/13/medline KW - Glucose metabolism KW - Glucose transporters KW - Insulin signaling KW - Myricetin SP - 173 EP - 85 JF - Toxicology and applied pharmacology JO - Toxicol Appl Pharmacol VL - 279 IS - 2 N2 - Diabetic nephropathy is the kidney disease that occurs as a result of diabetes. The present study was aimed to evaluate the therapeutic potential of myricetin by assaying the activities of key enzymes of carbohydrate metabolism, insulin signaling molecules and renal function markers in streptozotocin (STZ)-cadmium (Cd) induced diabetic nephrotoxic rats. After myricetin treatment schedule, blood and tissue samples were collected to determine plasma glucose, insulin, hemoglobin, glycosylated hemoglobin and renal function markers, carbohydrate metabolic enzymes in the liver and insulin signaling molecules in the pancreas and skeletal muscle. A significant increase of plasma glucose, glycosylated hemoglobin, urea, uric acid, creatinine, blood urea nitrogen (BUN), urinary albumin, glycogen phosphorylase, glucose-6-phosphatase, and fructose-1,6-bisphosphatase and a significant decrease of plasma insulin, hemoglobin, hexokinase, glucose-6-phosphate dehydrogenase, glycogen and glycogen synthase with insulin signaling molecule expression were found in the STZ-Cd induced diabetic nephrotoxic rats. The administration of myricetin significantly normalizes the carbohydrate metabolic products like glucose, glycated hemoglobin, glycogen phosphorylase and gluconeogenic enzymes and renal function markers with increase insulin, glycogen, glycogen synthase and insulin signaling molecule expression like glucose transporter-2 (GLUT-2), glucose transporter-4 (GLUT-4), insulin receptor-1 (IRS-1), insulin receptor-2 (IRS-2) and protein kinase B (PKB). Based on the data, the protective effect of myricetin was confirmed by its histological annotation of the pancreas, liver and kidney tissues. These findings suggest that myricetin improved carbohydrate metabolism which subsequently enhances glucose utilization and renal function in STZ-Cd induced diabetic nephrotoxic rats. SN - 1096-0333 UR - https://www.unboundmedicine.com/medline/citation/24923654/Protective_effect_of_bioflavonoid_myricetin_enhances_carbohydrate_metabolic_enzymes_and_insulin_signaling_molecules_in_streptozotocin_cadmium_induced_diabetic_nephrotoxic_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0041-008X(14)00211-7 DB - PRIME DP - Unbound Medicine ER -