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Mini-FLOTAC, Kato-Katz and McMaster: three methods, one goal; highlights from north Argentina.
Parasit Vectors. 2014 Jun 14; 7:271.PV

Abstract

BACKGROUND

Copro-parasitological diagnosis is still a challenge in management of helminth infections at individual and community levels in resource-limited settings.The aim of our study was to compare the performance of three quantitative techniques: Kato-Katz, McMaster and Mini-FLOTAC methids. The study was carried out in Oran, Northern Argentina.

METHODS

200 schoolchildren were enrolled to provide a single stool sample, which was tested for helminth infections with Kato-Katz, McMaster and Mini-FLOTAC methods. The Mini-FLOTAC was performed with two flotation solutions (FS2 saturated saline and FS7 zinc sulphate). Preparation and reading time for each of the three methods was calculated both when processing single and multiple samples.

RESULTS

Out of 193 schoolchildren examined, 40% were positive for any helminth infection by any method; the most prevalent was Hymenolepis nana (23%) followed by Ascaris lumbricoides (17%) and a third group of less prevalent helminths: Enterobius vermicularis, Trichuris trichiura and hookworms (11% all together). Mini-FLOTAC FS2 was more sensitive than FS7 for H. nana (93% vs 78%) and for other helminths (85% vs 80%), whereas FS7 was more sensitive for A. lumbricoides (87% vs 61%). Kato-Katz method was more sensitive than McMaster method for A. lumbricoides (84% vs 48%) and for other helminths (48% vs 43%) except for H. nana (49% vs 61%). As for egg counts, Mini-FLOTAC FS2 reported 904 eggs per gram of faeces (EPG) for H. nana (vs 457 with McMaster and 111 with Kato-Katz) and 1177 EPG for A. lumbricoides (vs 1315 with Kato-Katz and 995 with McMaster); FS2 detected the highest EPG for both H.nana and A.lumbricoides (904 vs 568 and 1177 vs 643 respectively), the differences were not statistically significant. The technique feasibility was calculated: Kato-Katz mean time was 48 minutes/sample, Mini-FLOTAC 13 minutes/sample and McMaster 7 minutes/sample. However, especially for Kato-Katz and Mini-FLOTAC, the mean time (min/sample) decreased significantly when processing multiple samples.

CONCLUSIONS

Mini-FLOTAC is a promising technique for helminth diagnosis, it is more sensitive than Kato-Katz and McMaster for H. nana and as sensitive as Kato-Katz and more sensitive than McMaster for A. lumbricoides identification. Egg counts differences although relevant, did not reach statistical significance.

Authors+Show Affiliations

Laboratory of Microbiology San Raffaele Hospital, Milan, Italy. Beatrice.barda@gmail.com.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

24929554

Citation

Barda, Beatrice, et al. "Mini-FLOTAC, Kato-Katz and McMaster: Three Methods, One Goal; Highlights From North Argentina." Parasites & Vectors, vol. 7, 2014, p. 271.
Barda B, Cajal P, Villagran E, et al. Mini-FLOTAC, Kato-Katz and McMaster: three methods, one goal; highlights from north Argentina. Parasit Vectors. 2014;7:271.
Barda, B., Cajal, P., Villagran, E., Cimino, R., Juarez, M., Krolewiecki, A., Rinaldi, L., Cringoli, G., Burioni, R., & Albonico, M. (2014). Mini-FLOTAC, Kato-Katz and McMaster: three methods, one goal; highlights from north Argentina. Parasites & Vectors, 7, 271. https://doi.org/10.1186/1756-3305-7-271
Barda B, et al. Mini-FLOTAC, Kato-Katz and McMaster: Three Methods, One Goal; Highlights From North Argentina. Parasit Vectors. 2014 Jun 14;7:271. PubMed PMID: 24929554.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mini-FLOTAC, Kato-Katz and McMaster: three methods, one goal; highlights from north Argentina. AU - Barda,Beatrice, AU - Cajal,Pamela, AU - Villagran,Eliana, AU - Cimino,Ruben, AU - Juarez,Marisa, AU - Krolewiecki,Alejandro, AU - Rinaldi,Laura, AU - Cringoli,Giuseppe, AU - Burioni,Roberto, AU - Albonico,Marco, Y1 - 2014/06/14/ PY - 2013/12/04/received PY - 2014/05/26/accepted PY - 2014/6/16/entrez PY - 2014/6/16/pubmed PY - 2015/2/5/medline SP - 271 EP - 271 JF - Parasites & vectors JO - Parasit Vectors VL - 7 N2 - BACKGROUND: Copro-parasitological diagnosis is still a challenge in management of helminth infections at individual and community levels in resource-limited settings.The aim of our study was to compare the performance of three quantitative techniques: Kato-Katz, McMaster and Mini-FLOTAC methids. The study was carried out in Oran, Northern Argentina. METHODS: 200 schoolchildren were enrolled to provide a single stool sample, which was tested for helminth infections with Kato-Katz, McMaster and Mini-FLOTAC methods. The Mini-FLOTAC was performed with two flotation solutions (FS2 saturated saline and FS7 zinc sulphate). Preparation and reading time for each of the three methods was calculated both when processing single and multiple samples. RESULTS: Out of 193 schoolchildren examined, 40% were positive for any helminth infection by any method; the most prevalent was Hymenolepis nana (23%) followed by Ascaris lumbricoides (17%) and a third group of less prevalent helminths: Enterobius vermicularis, Trichuris trichiura and hookworms (11% all together). Mini-FLOTAC FS2 was more sensitive than FS7 for H. nana (93% vs 78%) and for other helminths (85% vs 80%), whereas FS7 was more sensitive for A. lumbricoides (87% vs 61%). Kato-Katz method was more sensitive than McMaster method for A. lumbricoides (84% vs 48%) and for other helminths (48% vs 43%) except for H. nana (49% vs 61%). As for egg counts, Mini-FLOTAC FS2 reported 904 eggs per gram of faeces (EPG) for H. nana (vs 457 with McMaster and 111 with Kato-Katz) and 1177 EPG for A. lumbricoides (vs 1315 with Kato-Katz and 995 with McMaster); FS2 detected the highest EPG for both H.nana and A.lumbricoides (904 vs 568 and 1177 vs 643 respectively), the differences were not statistically significant. The technique feasibility was calculated: Kato-Katz mean time was 48 minutes/sample, Mini-FLOTAC 13 minutes/sample and McMaster 7 minutes/sample. However, especially for Kato-Katz and Mini-FLOTAC, the mean time (min/sample) decreased significantly when processing multiple samples. CONCLUSIONS: Mini-FLOTAC is a promising technique for helminth diagnosis, it is more sensitive than Kato-Katz and McMaster for H. nana and as sensitive as Kato-Katz and more sensitive than McMaster for A. lumbricoides identification. Egg counts differences although relevant, did not reach statistical significance. SN - 1756-3305 UR - https://www.unboundmedicine.com/medline/citation/24929554/Mini_FLOTAC_Kato_Katz_and_McMaster:_three_methods_one_goal L2 - https://parasitesandvectors.biomedcentral.com/articles/10.1186/1756-3305-7-271 DB - PRIME DP - Unbound Medicine ER -